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Endocrinology Vol. 138, No. 12 5637-5640
Copyright © 1997 by The Endocrine Society

ARTICLES

Effect of Oxytocin on Concentrations of Prostaglandin H Synthase-2 mRNA in Ovine Endometrial Tissue in Vivo

P. D. Burns, S.-J. Tsai, M. C. Wiltbank, S. H. Hayes, G. A. Graf and W. J. Silvia

Department of Animal Sciences and Center for Membrane Sciences (P.D.B., S.H.H., G.A.G., W.J.S.), University of Kentucky, Lexington, Kentucky 40546-0215; and Endocrinology-Reproductive Physiology Program (S.-J.T., M.C.W.), University of Wisconsin, Madison, Wisconsin 54706-1284

Address all correspondence and requests for reprints to: William J. Silvia, Ph.D., M.S., University of Kentucky, Department of Animal Sciences, 405 WP Garrigus Buliding, Lexington, Kentucky 40546-0215.

Oxytocin is an acute stimulus of prostaglandin (PG) F2{alpha} secretion from the ovine uterine endometrium. The high level of PGF2{alpha} secretion induced by oxytocin and the short half-life of the prostaglandin synthase-2 (PGHS-2) enzyme implies that synthesis of PGHS-2 may be essential at this time. The objective of this study was to determine if the increase in PGF2{alpha} secretion induced by oxytocin is associated with an increase in PGHS-2 mRNA. In experiment 1, oxytocin induced a rapid increase in serum concentration of 13,14-dihydro-15-keto-prostaglandin F2{alpha} (the stable metabolite of PGF2{alpha}; PGFM) that was detected within 7.5 min (P < 0.05) and peaked at 25 min post injection. This was associated with an unusually rapid increase in the concentration of PGHS-2 mRNA at 25 min post oxytocin injection (P < 0.05). Endometrial concentrations of PGHS-2 mRNA returned to basal levels at 90 min post injection. Experiment 2 was conducted to further characterize the time course of induction of PGHS-2 mRNA following oxytocin administration. Oxytocin induced a rapid increase in serum concentrations of PGFM. As in experiment 1, an increase in concentrations of PGHS-2 mRNA was detected at 25 min after oxytocin (P = 0.06). Concentrations of PGHS-2 mRNA were intermediate at 40 min and returned to basal levels at 60 min post injection. Thus, there is a rapid increase in endometrial concentrations of PGHS-2 mRNA following oxytocin stimulation of PGF2{alpha} secretion. This increase in PGHS-2 mRNA may be required to maintain PGHS-2 enzyme levels during pulsatile secretion of PGF2{alpha} at luteolysis.




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