help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bodart, V.
Right arrow Articles by De Léan, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bodart, V.
Right arrow Articles by De Léan, A.
Endocrinology Vol. 138, No. 2 566-573
Copyright © 1997 by The Endocrine Society

Articles

Comparative Effect of Pituitary Adenylate Cyclase-Activating Polypeptide on Aldosterone Secretion in Normal Bovine and Human Tumorous Adrenal Cells1

V. Bodart, K. Babinski, H. Ong and A. De Léan2

Faculty of Pharmacy, Department of Pharmacology, Faculty of Medicine, Université de Montréal, Montréal, Québec H3C 3J7, Canada

Address all correspondence and requests for reprints to: Dr. A. De Léan, Department of Pharmacology, Faculty of Medicine, Université de Montréal, Case Postale 6128, Succursale Centre-Ville, Montréal H3C 3J7, Canada. E-mail: delean{at}ere.umontreal.ca

The purpose of this study was to investigate the mechanisms of action of pituitary adenylate cyclase-activating polypeptide (PACAP) in stimulating aldosterone production in two different models: bovine adrenal zona glomerulosa (ZG) cells in primary culture and the human adrenocortical carcinoma cell line H295R. PACAP binds to two major groups of receptors: type I, which prefers PACAP38 and PACAP27 over vasoactive intestinal peptide (VIP); and type II, which has approximately equal affinity for PACAP38, PACAP27, and VIP. The type I subclass comprises multiple splice variants that can be distinguished by their specificity to PACAP38 and PACAP27 in their activation of adenylate cyclase and phospholipase C. Type II PACAP/VIP receptors couple only to AC. In bovine ZG cells, PACAP38 and PACAP27 stimulated aldosterone production in a dose-dependent manner, whereas VIP was ineffective. In H295R cells, PACAP38, PACAP27, and VIP dose-dependently stimulated aldosterone production with roughly the same ED50. In bovine ZG cells, PACAP38 and PACAP27 stimulated cAMP production with similar efficacy, whereas VIP had no effect. In H295R cells, all three peptides stimulated cAMP accumulation. PACAP38 and PACAP27 also activated PLC in bovine ZG cells as they induced an increase in Ins(1,4,5)P3 production. In H295R cells, neither of these peptides was able to stimulate IP turnover. These results indicate that PACAP stimulation of aldosterone production is mediated by the PVR1s or the PVR1hop splice variants of the type I PACAP-specific receptor subtype in bovine ZG cells, whereas only type II PACAP/VIP receptors seemed to occur in the human H295R cell line. In addition, PACAP-stimulated aldosterone production was inhibited by atrial natriuretic peptide in bovine and human adrenocortical cells, however not by the same mechanism. This further supports species-specific and/or cell type-specific signaling pathways for PACAP in the regulation of aldosterone production.




This article has been cited by other articles:


Home page
 J EndocrinolHome page
S. Valiante, M. Prisco, R. Sciarrillo, M. De Falco, A. Capaldo, F. Gay, P. Andreuccetti, V. Laforgia, and L. Varano
Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland
J. Endocrinol., February 1, 2008; 196(2): 291 - 303.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Mazzocchi, L. K. Malendowicz, P. Rebuffat, L. Gottardo, and G. G. Nussdorfer
Expression and Function of Vasoactive Intestinal Peptide, Pituitary Adenylate Cyclase-Activating Polypeptide, and Their Receptors in the Human Adrenal Gland
J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2575 - 2580.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
N. M. Sherwood, S. L. Krueckl, and J. E. McRory
The Origin and Function of the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)/Glucagon Superfamily
Endocr. Rev., December 1, 2000; 21(6): 619 - 670.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
S. Gräs, C. Hedetoft, S. H. Pedersen, and J. Fahrenkrug
Pituitary Adenylate Cyclase-Activating Peptide Stimulates Acute Progesterone Production in Rat Granulosa/Lutein Cells via Two Receptor Subtypes
Biol Reprod, July 1, 2000; 63(1): 206 - 212.
[Abstract] [Full Text]

Home page
 Pharmacol. Rev.Home page
D. Vaudry, B. J. Gonzalez, M. Basille, L. Yon, A. Fournier, and H. Vaudry
Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: From Structure to Functions
Pharmacol. Rev., June 1, 2000; 52(2): 269 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. Ehrhart-Bornstein, J. P. Hinson, S. R. Bornstein, W. A. Scherbaum, and G. P. Vinson
Intraadrenal Interactions in the Regulation of Adrenocortical Steroidogenesis
Endocr. Rev., April 1, 1998; 19(2): 101 - 143.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society