This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Valverde-R, C. Articles by St. Germain, D. L. Search for Related Content PubMed PubMed Citation Articles by Valverde-R, C. Articles by St. Germain, D. L. Pubmed/NCBI databases Protein UniGene Substance via MeSH

Cloning and Expression of a 5'-Iodothyronine Deiodinase from the Liver of Fundulus heteroclitus¹

Universidad National Autonoma de Mexico (C.V.-R., A.O.), Mexico 04510 D. F.; Departments of Medicine and Physiology (W.C., D.L.S.), Dartmouth Medical School, Lebanon, New Hampshire 03756; and The Whitney Laboratory (G.J.L.), University of Florida, St. Augustine, Florida 32086

Address all correspondence and requests for reprints to: Donald L. St. Germain, M.D., Dartmouth Medical School, One Medical Center Drive, Lebanon, New Hampshire 03756. E-mail: stgermain{at}dartmouth.edu

Recent molecular cloning studies in mammals and amphibians have demonstrated that the types I, II, and III deiodinases constitute a family of selenoproteins of critical importance in metabolizing T₄ to active (i.e. T₃) and inactive (i.e. rT₃) metabolites. In several tissues of teleost fish, various deiodinase processes have been described, but the structural and functional characteristics of these enzymes and their relationship to the deiodinases present in higher vertebrates remains uncertain. Using a complementary DNA library derived from the liver of the teleost Fundulus heteroclitus, we have identified a complementary DNA that codes for a deiodinase with functional characteristics virtually identical to those of the mammalian and amphibian type II deiodinase. Sequence analysis demonstrates a high degree of homology at both the nucleotide and predicted amino acid levels between the Fundulus clone and these previously characterized type II enzymes, including the presence of an in-frame TGA codon that codes for selenocysteine. These findings demonstrate that the deiodinase family of selenoproteins has been highly conserved during vertebrate evolution and underscores their importance in the regulation of thyroid hormone action.

This article has been cited by other articles:

				S Van der Geyten, N Byamungu, G E Reyns, E R Kuhn, and V M Darras Iodothyronine deiodinases and the control of plasma and tissue thyroid hormone levels in hyperthyroid tilapia (Oreochromis niloticus) J. Endocrinol., March 1, 2005; 184(3): 467 - 479. [Abstract] [Full Text] [PDF]

				C. A. Shepherdley, W. Klootwijk, K. W. Makabe, T. J. Visser, and G. G. J. M. Kuiper An Ascidian Homolog of Vertebrate Iodothyronine Deiodinases Endocrinology, March 1, 2004; 145(3): 1255 - 1268. [Abstract] [Full Text] [PDF]

				G. G. J. M. Kuiper, W. Klootwijk, and T. J. Visser Substitution of Cysteine for a Conserved Alanine Residue in the Catalytic Center of Type II Iodothyronine Deiodinase Alters Interaction with Reducing Cofactor Endocrinology, April 1, 2002; 143(4): 1190 - 1198. [Abstract] [Full Text] [PDF]

				A. C. Bianco, D. Salvatore, B. Gereben, M. J. Berry, and P. R. Larsen Biochemistry, Cellular and Molecular Biology, and Physiological Roles of the Iodothyronine Selenodeiodinases Endocr. Rev., February 1, 2002; 23(1): 38 - 89. [Abstract] [Full Text] [PDF]

				B. Gereben, D. Salvatore, J. W. Harney, H. M. Tu, and P. R. Larsen The Human, but Not Rat, dio2 Gene Is Stimulated by Thyroid Transcription Factor-1 (TTF-1) Mol. Endocrinol., January 1, 2001; 15(1): 112 - 124. [Abstract] [Full Text]

				J. P. Sanders, S. Van der Geyten, E. Kaptein, V. M. Darras, E. R. Kühn, J. L. Leonard, and T. J. Visser Cloning and Characterization of Type III Iodothyronine Deiodinase from the Fish Oreochromis niloticus Endocrinology, August 1, 1999; 140(8): 3666 - 3673. [Abstract] [Full Text]

				N. Montuori, F. Müller, S. De Riu, G. Fenzi, M. E. Sobel, G. Rossi, and M. Vitale Laminin Receptors in Differentiated Thyroid Tumors: Restricted Expression of the 67-Kilodalton Laminin Receptor in Follicular Carcinoma Cells J. Clin. Endocrinol. Metab., June 1, 1999; 84(6): 2086 - 2092. [Abstract] [Full Text]

				B. Gereben, T. Bartha, H. M. Tu, J. W. Harney, P. Rudas, and P. R. Larsen Cloning and Expression of the Chicken Type 2 Iodothyronine 5'-Deiodinase J. Biol. Chem., May 14, 1999; 274(20): 13768 - 13776. [Abstract] [Full Text] [PDF]

				S. Van der Geyten, J. P. Sanders, E. Kaptein, V. M. Darras, E. R. Kuhn, J. L. Leonard, and T. J. Visser Expression of Chicken Hepatic Type I and Type III Iodothyronine Deiodinases during Embryonic Development Endocrinology, December 1, 1997; 138(12): 5144 - 5152. [Abstract] [Full Text] [PDF]

				J. P. Sanders, S. Van der Geyten, E. Kaptein, V. M. Darras, E. R. Kuhn, J. L. Leonard, and T. J. Visser Characterization of a Propylthiouracil-Insensitive Type I Iodothyronine Deiodinase Endocrinology, December 1, 1997; 138(12): 5153 - 5160. [Abstract] [Full Text] [PDF]

				S. Pallud, A.-M. Lennon, M. Ramauge, J.-M. Gavaret, W. Croteau, M. Pierre, F. Courtin, and D. L. St. Germain Expression of the Type II Iodothyronine Deiodinase in Cultured Rat Astrocytes Is Selenium-dependent J. Biol. Chem., July 18, 1997; 272(29): 18104 - 18110. [Abstract] [Full Text] [PDF]