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Endocrinology Vol. 138, No. 2 771-777
Copyright © 1997 by The Endocrine Society

Articles

Induction of c-fos Messenger Ribonucleic Acid in Neuropeptide Y and Growth Hormone (GH)-Releasing Factor Neurons in the Rat Arcuate Nucleus Following Systemic Injection of the GH Secretagogue, GH-Releasing Peptide-61

Suzanne L. Dickson and Simon M. Luckman2

Anatomy and Human Biology Group, King’s College London (S.L.D.), The Strand, London WC2R 2LS; and Department of Neurobiology, The Babraham Institute (S.M.L.), Babraham, Cambridge CB2 4AT, United Kingdom

Address all correspondence and requests for reprints to: Suzanne L. Dickson, The Department of Physiology, University of Cambridge, Downing Street, Cambridge CB2 3EG, United Kingdom. E-mail: sld20{at}cam.ac.uk

In this study we investigated the neurochemical identity of the arcuate cells activated following GH-releasing peptide-6 (GHRP-6) injection by comparing, on consecutive sections, the distribution c-fos messenger RNA (mRNA) with that of mRNAs for peptides synthesized in arcuate cells, including neuropeptide Y (NPY), GH-releasing factor (GRF), tyrosine hydroxylase, POMC, and somatostatin. Rats bearing chronically implanted jugular catheters were injected with either 50 µg GHRP-6 or vehicle. Thirty minutes later they were terminally anesthetized and perfused with fixative. Paraffin-embedded sections of 7 µm thickness were processed using in situ hybridization for either c-fos mRNA or mRNAs for the neurochemical markers. In GHRP-6-treated rats the mean (± SEM) number of cells expressing c-fos mRNA in the arcuate nucleus (23 ± 2 cells/section per rat; n = 5) was significantly higher than for vehicle-treated controls (2 ± 1 cells/section per rat; n = 5; P < 0.001, Mann-Whitney U test). Superimposed camera lucida maps indicated that, in GHRP-6-injected rats, neurochemically identifiable cells expressing c-fos mRNA also express NPY mRNA (51 ± 4%), GRF mRNA (23 ± 1%) tyrosine hydroxylase mRNA (11 ± 3%), POMC mRNA (11 ± 2%), or somatostatin mRNA (4 ± 1%). Thus, the majority of cells expressing c-fos mRNA following GHRP-6 injection are NPY and GRF-containing cells.




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