| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Articles |
Departments of Research and Medicine, Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105; and University of Connecticut School of Medicine, Farmington, Connecticut 06030
Address all correspondence and requests for reprints to: Samuel Varghese, Ph.D., Department of Research, Saint Francis Hospital and Medical Center, 114 Woodland Street, Hartford, Connecticut 06105-1299.
Bone morphogenetic protein-2 (BMP-2), a member of the transforming growth factor superfamily of peptides, induces ectopic bone formation in vivo. The actions of BMP-2 on osteoblastic cells include stimulation of collagen synthesis, but the role of BMP-2 on collagen degradation is not known. We examined whether BMP-2 affects the expression of collagenase-3, an enzyme that degrades type I collagen at neutral pH, and that of tissue inhibitors of matrix metalloproteinases (TIMPs) in primary osteoblast-enriched cells from 22-day-old fetal rat calvariae. BMP-2 suppressed collagenase messenger RNA (mRNA) and immunoreactive protein levels. BMP-2 did not affect collagenase mRNA stability, but it reduced collagenase heterogeneous nuclear RNA levels and decreased the rate of transcription of the collagenase gene. BMP-2 also stimulated TIMP 1 and TIMP 3 mRNA levels, but failed to alter TIMP 2 expression. In conclusion, our studies indicate that BMP-2 suppresses collagenase-3 gene transcription and stimulates TIMP 1 and TIMP 3 expression in osteoblasts. The regulation of collagenase and TIMPs by BMP-2 in osteoblasts may play a role in osteoinduction.
This article has been cited by other articles:
 |
|
 |
|
  E. Gazzerro, R. C. Pereira, V. Jorgetti, S. Olson, A. N. Economides, and E. Canalis Skeletal Overexpression of Gremlin Impairs Bone Formation and Causes Osteopenia Endocrinology, February 1, 2005; 146(2): 655 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Im, C. Pacione, S. Chubinskaya, A. J. van Wijnen, Y. Sun, and R. F. Loeser Inhibitory Effects of Insulin-like Growth Factor-1 and Osteogenic Protein-1 on Fibronectin Fragment- and Interleukin-1{beta}-stimulated Matrix Metalloproteinase-13 Expression in Human Chondrocytes J. Biol. Chem., July 3, 2003; 278(28): 25386 - 25394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Canalis, A. N. Economides, and E. Gazzerro Bone Morphogenetic Proteins, Their Antagonists, and the Skeleton Endocr. Rev., April 1, 2003; 24(2): 218 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Pereira and E. Canalis Parathyroid Hormone Increases mac25/Insulin-Like Growth Factor-Binding Protein-Related Protein-1 Expression in Cultured Osteoblasts Endocrinology, May 1, 1999; 140(5): 1998 - 2003. [Abstract] [Full Text]
|
|
|
|
|
|
E. Gazzerro, S. Rydziel, and E. Canalis Skeletal Bone Morphogenetic Proteins Suppress the Expression of Collagenase-3 by Rat Osteoblasts Endocrinology, February 1, 1999; 140(2): 562 - 567. [Abstract] [Full Text]
|
|
|
|
|
|
N. Franchimont, S. Rydziel, A. M. Delany, and E. Canalis Interleukin-6 and Its Soluble Receptor Cause a Marked Induction of Collagenase 3 Expression in Rat Osteoblast Cultures J. Biol. Chem., May 2, 1997; 272(18): 12144 - 12150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Winchester, N. Selvamurugan, R. C. D'Alonzo, and N. C. Partridge Developmental Regulation of Collagenase-3 mRNA in Normal, Differentiating Osteoblasts through the Activator Protein-1 and the runt Domain Binding Sites J. Biol. Chem., July 21, 2000; 275(30): 23310 - 23318. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
