help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gil, B.
Right arrow Articles by Alvarez, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gil, B.
Right arrow Articles by Alvarez, L.
Endocrinology Vol. 138, No. 3 1251-1258
Copyright © 1997 by The Endocrine Society

Articles

Glucocorticoid Regulation of Hepatic S-Adenosylmethionine Synthetase Gene Expression1

Beatriz Gil2, María A. Pajares, José M. Mato and Luis Alvarez

Institute of Biomedical Investigation, Consejo Superior de Investigaciones Cientificas, Madrid, Spain

Address all correspondence and requests for reprints to: Dr. José M. Mato, Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Cientificas, Arturo Duperier 4, 28029 Madrid, Spain. E-mail: jmmato{at}biomed.iib.uam.es

The effects of glucocorticoids on the regulation of rat liver S-adenosylmethionine synthetase were studied in vivo and in two culture systems. Livers from adrenalectomized animals were examined for enzyme activity, immunoreactive protein, and messenger RNA (mRNA) content. All three parameters showed a similar trend, i.e. they decreased 3-fold after adrenalectomy and increased over the control values upon triamcinolone replacement. These results suggested that glucocorticoid regulation of hepatic S-adenosylmethionine synthetase was mediated at the mRNA level. Triamcinolone and dexamethasone increased S-adenosylmethionine synthetase mRNA content in a time- and dose-dependent manner in both rat hepatoma H35 cells and primary cultures of adult rat hepatocytes. The kinetics of mRNA induction were identical in both culture systems, indicating that the hormone-mediated response is independent of the differentiated state of the cell. Insulin blocked the inducing effect of glucocorticoids on S-adenosylmethionine synthetase mRNA in a dose-dependent manner. On the other hand, the triamcinolone-dependent increase in mRNA levels was completely abolished by treatment with actinomycin D, whereas cycloheximide did not affect this response. The transcription rate of the gene, as measured by run-on assay, increased 3-fold after hormone addition. Transient transfections of H35 cells with 1.4 kilobases of the 5'-flanking region of the hepatic S-adenosylmethionine synthetase gene fused to a luciferase reporter gene showed that promoter activity is also increased 3-fold after triamcinolone treatment, suggesting that this promoter region contains the sequence elements necessary to confer glucocorticoid responsiveness. In addition to the transcriptional control of the hepatic S-adenosylmethionine synthetase gene, our results suggest that glucocorticoids may be acting at a posttranscriptional level.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Ratnam, E. P. Wijekoon, B. Hall, T. A. Garrow, M. E. Brosnan, and J. T. Brosnan
Effects of diabetes and insulin on betaine-homocysteine S-methyltransferase expression in rat liver
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E933 - E939.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
E. P. Wijekoon, B. Hall, S. Ratnam, M. E. Brosnan, S. H. Zeisel, and J. T. Brosnan
Homocysteine Metabolism in ZDF (Type 2) Diabetic Rats
Diabetes, November 1, 2005; 54(11): 3245 - 3251.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. L. Ulrey, L. Liu, L. G. Andrews, and T. O. Tollefsbol
The impact of metabolism on DNA methylation
Hum. Mol. Genet., April 15, 2005; 14(suppl_1): R139 - R147.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Nieman, M. J. Rowling, T. A. Garrow, and K. L. Schalinske
Modulation of Methyl Group Metabolism by Streptozotocin-induced Diabetes and All-trans-retinoic Acid
J. Biol. Chem., October 29, 2004; 279(44): 45708 - 45712.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
I. Iloro, R. Chehin, F. M. Goni, M. A. Pajares, and J.-L. R. Arrondo
Methionine Adenosyltransferase {alpha}-Helix Structure Unfolds at Lower Temperatures than {beta}-Sheet: A 2D-IR Study
Biophys. J., June 1, 2004; 86(6): 3951 - 3958.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. J. Rowling and K. L. Schalinske
Retinoic Acid and Glucocorticoid Treatment Induce Hepatic Glycine N-Methyltransferase and Lower Plasma Homocysteine Concentrations in Rats and Rat Hepatoma Cells
J. Nutr., November 1, 2003; 133(11): 3392 - 3398.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. F. Sanchez-Perez, M. Gasset, J. J. Calvete, and M. A. Pajares
Role of an Intrasubunit Disulfide in the Association State of the Cytosolic Homo-oligomer Methionine Adenosyltransferase
J. Biol. Chem., February 21, 2003; 278(9): 7285 - 7293.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. M. MATO, F. J. CORRALES, S. C. LU, and M. A. AVILA
S-Adenosylmethionine: a control switch that regulates liver function
FASEB J, January 1, 2002; 16(1): 15 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Oscarsson, C. Gardmo, S. Eden, and A. Mode
Pulsatile growth hormone secretion decreases S-adenosylmethionine synthetase in rat liver
Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E280 - E286.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. R. GARCÍA-TREVIJANO, M. U. LATASA, M. V. CARRETERO, C. BERASAIN, J. M. MATO, and M. A. AVILA
S-Adenosylmethionine regulates MAT1A and MAT2A gene expression in cultured rat hepatocytes: a new role for S-adenosylmethionine in the maintenance of the differentiated status of the liver
FASEB J, December 1, 2000; 14(15): 2511 - 2518.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
L. I. McKay and J. A. Cidlowski
Molecular Control of Immune/Inflammatory Responses: Interactions Between Nuclear Factor-{kappa}B and Steroid Receptor-Signaling Pathways
Endocr. Rev., August 1, 1999; 20(4): 435 - 459.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Z.-Z. Huang, Z. Mao, J. Cai, and S. C. Lu
Changes in methionine adenosyltransferase during liver regeneration in the rat
Am J Physiol Gastrointest Liver Physiol, July 1, 1998; 275(1): G14 - G21.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Alvarez, E. Sanchez-Gongora, J. Mingorance, M. A. Pajares, and J. M. Mato
Characterization of Rat Liver-specific Methionine Adenosyltransferase Gene Promoter. ROLE OF DISTAL UPSTREAM cis-ACTING ELEMENTS IN THE REGULATION OF THE TRANSCRIPTIONAL ACTIVITY
J. Biol. Chem., September 5, 1997; 272(36): 22875 - 22883.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society