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Decreased Hepatic Insulin-Like Growth Factor (IGF)-I and Increased IGF Binding Protein-1 and -2 Gene Expression in Experimental Uremia

Department of Pediatrics and Physiology, State University of New York at Stony Brook (B.T., D.Z., L.C.M., F.J.K.), Stony Brook, New York 11794; Department of Pediatrics, Baylor College of Medicine Houston (D.R.P., S.K.D., M.E.C.), Texas 77030; Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health (H.M.D.), Bethesda, Maryland 20892; University Children’s Hospital, Gießen 35385 (W.F.B.), Germany; and Kolling Institute of Medical Research (R.C.B.), St. Leonards, NSW 2065, Australia

Address all correspondence and requests for reprints to: Burkhard Tönshoff, Division of Pediatric Nephrology, University Children’s Hospital, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany. E-mail: Burkhard-Toenshoff{at}krzmail.krz.uni-heidelberg.de

The imbalance between normal insulin-like growth factor-I (IGF-I) and markedly increased IGF binding protein (IGFBP) plasma levels plays a pathogenic role for growth retardation and catabolism in children with chronic renal failure. To investigate the mechanism of these alterations, experiments were performed in an experimental model of uremia in rats (5/6 nephrectomy) and in pair-fed and ad libitum-fed sham-operated controls. Using a specific solution hybridization/RNase protection assay, we observed a marked reduction of hepatic IGF-I messenger RNA (mRNA) abundance at steady state in uremic animals (37 ± 5% of control) compared both with pair-fed (65 ± 10%) and ad libitum-fed controls (100 ± 11%) (P < 0.001). Reduced IGF-I gene expression was clearly organ-specific; it was most pronounced in liver (significant vs.. pair-fed controls) and lung and muscle tissue (significant vs.. ad libitum-fed controls); no change was observed in kidney and heart tissue. To determine a potential mechanism of reduced hepatic IGF-I gene expression in uremia, the hepatic GH receptor gene expression in the same experimental animals was analyzed by specific solution hybridization/RNase protection assay. Uremic animals had a 20–30% reduction of hepatic GH receptor mRNA abundance compared with controls. Hepatic GHBP expression in uremia was decreased in parallel. Despite the reduction of hepatic IGF-I mRNA abundance, plasma IGF-I levels in uremia were not different from ad libitum-fed controls. This discrepancy is explained by an increased concentration of IGFBPs in uremic plasma. By RIA, plasma IGFBP-1 levels in uremia were increased 4-fold; by Western immunoblot, plasma IGFBP-2 levels were increased 7-fold and plasma IGFBP-4 levels were increased 2-fold compared with both control groups. Intact IGFBP-3 (M_r, 48 kDa) and low molecular IGFBP-3 fragments were not significantly different among the three groups. By Northern blot analysis, hepatic IGFBP-1 mRNA levels in uremia were 2-fold higher than in controls. IGFBP-2 mRNA abundance in liver tissue was increased 4-fold, whereas in kidney there was a significant reduction of IGFBP-2 mRNA (30% of control). IGFBP-4 mRNA was increased by 50% in kidney but not in liver. Plasma insulin and corticosterone levels were not different among the groups. Our study shows that hepatic IGF-I gene expression was specifically reduced in uremia, partially as the consequence of a reduced hepatic GH receptor gene expression. One of the mechanisms contributing to increased IGFBP levels in uremia is increased hepatic gene expression of IGFBP-1 and IGFBP-2. The imbalance between reduced hepatic IGF-I production and increased hepatic IGFBP-1 and -2 production is likely to play a pathogenic role for catabolism and growth failure in CRF.

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