This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nehar, D. Articles by Benahmed, M. Search for Related Content PubMed PubMed Citation Articles by Nehar, D. Articles by Benahmed, M.

Tumor Necrosis Factor--Stimulated Lactate Production Is Linked to Lactate Dehydrogenase A Expression and Activity Increase in Porcine Cultured Sertoli Cells¹

INSERM U-407, Communications Cellulaires en Biologie de la Reproduction, Centre Hospitalier Lyon-Sud, Pierre-Benite, France

Address all correspondence and requests for reprints to: Dr. M. Benahmed, INSERM U-407, Communications Cellulaires en Biologie de la Reproduction, Bât 3 B, Centre Hospitalier Lyon-Sud, 69 495 Pierre-Benite, France.

By using, as a model, cultured testicular immature Sertoli cells, the action of tumor necrosis factor- (TNF) and the site of action of the cytokine on lactate production were studied. TNF stimulated in a time- and dose-dependent manner (with an ED₅₀ of 0.1 nM) Sertoli cell lactate production. Two major sites involved in TNF action were identified. Firstly, TNF was shown to increase the uptake of glucose substrate in a time- and dose-dependent manner. The maximal effect was observed after 24 h of treatment, with an ED₅₀ of 0.1 nM. Secondly, TNF increased the activity of lactate dehydrogenase (LDH) A isoform, which is involved in the conversion of pyruvate into lactate. This increase in LDH-A activity was detected at 12 h and was maximal after 24 h of treatment with TNF. The stimulatory effect of the cytokine on the LDH-A isoform was observed with an ED₅₀ of 0.05 nM. Such an increase in LDH-A activity was related to an increase in LDH-A expression, because TNF stimulated LDH-A messenger RNA (size, 1.5 kilobases, determined by Northern blotting analysis). Together, assuming that in the seminiferous tubules, TNF is produced by spermatids that use lactate for their energetic metabolism, we suggest that the cytokine may potentially represent a signal used by germ cells to enhance lactate production in Sertoli cells through, at least, a redistribution of LDH isoforms in favor of LDH-A.

This article has been cited by other articles:

				M. N. Galardo, M. F. Riera, E. H. Pellizzari, S. B. Cigorraga, and S. B. Meroni The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-D-ribonucleoside, regulates lactate production in rat Sertoli cells J. Mol. Endocrinol., October 1, 2007; 39(4): 279 - 288. [Abstract] [Full Text] [PDF]

				M. W M Li, W. Xia, D. D Mruk, C. Q F Wang, H. H N Yan, M. K Y Siu, W.-y. Lui, W. M Lee, and C Y. Cheng Tumor necrosis factor {alpha} reversibly disrupts the blood-testis barrier and impairs Sertoli-germ cell adhesion in the seminiferous epithelium of adult rat testes. J. Endocrinol., August 1, 2006; 190(2): 313 - 329. [Abstract] [Full Text] [PDF]

				C. RAE, J. A. MCQUILLAN, S. B. PAREKH, W. A. BUBB, S. WEISER, V. J. BALCAR, A. M. HANSEN, H. J. BALL, and N. H. HUNT Brain gene expression, metabolism, and bioenergetics: interrelationships in murine models of cerebral and noncerebral malaria FASEB J, March 1, 2004; 18(3): 499 - 510. [Abstract] [Full Text] [PDF]

				F. Boussouar, C. Mauduit, E. Tabone, L. Pellerin, P. J. Magistretti, and M. Benahmed Developmental and Hormonal Regulation of the Monocarboxylate Transporter 2 (MCT2) Expression in the Mouse Germ Cells Biol Reprod, September 1, 2003; 69(3): 1069 - 1078. [Abstract] [Full Text] [PDF]

				V. Pentikainen, K. Erkkila, L. Suomalainen, M. Otala, M. O. Pentikainen, M. Parvinen, and L. Dunkel TNF{alpha} Down-Regulates the Fas Ligand and Inhibits Germ Cell Apoptosis in the Human Testis J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4480 - 4488. [Abstract] [Full Text] [PDF]

				J. Miao, N. S. Panesar, K.-T. Chan, F. M.M. Lai, N.-s. Xia, Y.-b. Wang, P. J. Johnson, and J. Y.H. Chan Differential Expression of a Stress-modulating Gene, BRE, in the Adrenal Gland, in Adrenal Neoplasia, and in Abnormal Adrenal Tissues J. Histochem. Cytochem., April 1, 2001; 49(4): 491 - 500. [Abstract] [Full Text]

				R.;a. Grataroli, F. Boussouar, and M. Benahmed Role of Sphingosine in the Tumor Necrosis Factor {alpha} Stimulatory Effect on Lactate Dehydrogenase A Expression and Activity in Porcine Sertoli Cells Biol Reprod, November 1, 2000; 63(5): 1473 - 1481. [Abstract] [Full Text]

				F. Boussouar and M. Benahmed Epidermal Growth Factor Regulates Glucose Metabolism Through Lactate Dehydrogenase A Messenger Ribonucleic Acid Expression in Cultured Porcine Sertoli Cells Biol Reprod, October 1, 1999; 61(4): 1139 - 1145. [Abstract] [Full Text]

				F. Boussouar, R. Grataroli, J. Ji, and M. Benahmed Tumor Necrosis Factor-{alpha} Stimulates Lactate Dehydrogenase A Expression in Porcine Cultured Sertoli Cells: Mechanisms of Action Endocrinology, July 1, 1999; 140(7): 3054 - 3062. [Abstract] [Full Text]

				F. Delfino and W. H. Walker Stage-Specific Nuclear Expression of NF-{kappa}B in Mammalian Testis Mol. Endocrinol., November 1, 1998; 12(11): 1696 - 1707. [Abstract] [Full Text]