help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frenkel, B.
Right arrow Articles by Lian, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frenkel, B.
Right arrow Articles by Lian, J. B.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Endocrinology Vol. 138, No. 5 2109-2116
Copyright © 1997 by The Endocrine Society

Articles

Activity of the Osteocalcin Promoter in Skeletal Sites of Transgenic Mice and during Osteoblast Differentiation in Bone Marrow-Derived Stromal Cell Cultures: Effects of Age and Sex1

B. Frenkel, C. Capparelli, M. van Auken, D. Baran , J. Bryan, J. L. Stein, G. S. Stein and J. B. Lian

Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, Worcester, Massachusetts 01655

Address all correspondence and requests for reprints to authors at the Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, Worcester, Massachusetts 01655-0106.

The bone-specific osteocalcin gene is a well established marker of osteoblast activity. We have studied osteocalcin transcription in transgenic mice carrying rat osteocalcin promoter-chloramphenicol acetyltransferase (CAT) reporter constructs. Transgenic lines carrying each of the 1.7-, 1.1-, 0.72-, or 0.35-kilobase promoter constructs expressed the reporter gene in a tissue-specific manner. However, each of these constructs was sensitive to site of integration effects, reflected by a high frequency of nonexpressing transgenic lines. High expression of the 1.7-kilobase promoter in osseous tissues was accompanied by low ectopic expression in the brain. Analysis of CAT expression in femurs, calvariae, and lumbar vertebrae of this line indicated considerable variability in promoter activity among individual transgenic animals. Analysis of the variance in CAT activity demonstrated a linkage between promoter activities in these distant skeletal sites. Promoter activity was inversely correlated with age, and females exhibited severalfold higher activity than age-matched males. Bone marrow stromal cells from these animals, cultured under conditions that support osteoblast differentiation, exhibited the expected postproliferative onset of osteocalcin promoter activity, as assessed by CAT assay. The ex vivo CAT activity was not dependent on the sex or the age of the donor transgenic mouse. Taken together, our results are consistent with the hypothesis that a common, probably humoral, factor(s) regulates osteocalcin transcription in distant skeletal sites. We suggest that the abundance of this factor(s) is different between males and females and among individual mice at a given time point, and that ex vivo culturing of osteoblasts reduces the variation in osteocalcin promoter activity by eliminating the physiological contribution of this factor.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
A. Smerdel-Ramoya, S. Zanotti, L. Stadmeyer, D. Durant, and E. Canalis
Skeletal Overexpression of Connective Tissue Growth Factor Impairs Bone Formation and Causes Osteopenia
Endocrinology, September 1, 2008; 149(9): 4374 - 4381.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Zanotti, A. Smerdel-Ramoya, L. Stadmeyer, D. Durant, F. Radtke, and E. Canalis
Notch Inhibits Osteoblast Differentiation and Causes Osteopenia
Endocrinology, August 1, 2008; 149(8): 3890 - 3899.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Gazzerro, A. Smerdel-Ramoya, S. Zanotti, L. Stadmeyer, D. Durant, A. N. Economides, and E. Canalis
Conditional Deletion of Gremlin Causes a Transient Increase in Bone Formation and Bone Mass
J. Biol. Chem., October 26, 2007; 282(43): 31549 - 31557.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Rydziel, L. Stadmeyer, S. Zanotti, D. Durant, A. Smerdel-Ramoya, and E. Canalis
Nephroblastoma Overexpressed (Nov) Inhibits Osteoblastogenesis and Causes Osteopenia
J. Biol. Chem., July 6, 2007; 282(27): 19762 - 19772.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Ofek, M. Karsak, N. Leclerc, M. Fogel, B. Frenkel, K. Wright, J. Tam, M. Attar-Namdar, V. Kram, E. Shohami, et al.
Peripheral cannabinoid receptor, CB2, regulates bone mass
PNAS, January 17, 2006; 103(3): 696 - 701.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Smith, T. E. Meyerrose, T. Kohler, M. Namdar-Attar, N. Bab, O. Lahat, T. Noh, J. Li, M. W. Karaman, J. G. Hacia, et al.
Leaky ribosomal scanning in mammalian genomes: significance of histone H4 alternative translation in vivo
Nucleic Acids Res., March 1, 2005; 33(4): 1298 - 1308.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. Gazzerro, R. C. Pereira, V. Jorgetti, S. Olson, A. N. Economides, and E. Canalis
Skeletal Overexpression of Gremlin Impairs Bone Formation and Causes Osteopenia
Endocrinology, February 1, 2005; 146(2): 655 - 665.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Q. Hassan, A. Javed, M. I. Morasso, J. Karlin, M. Montecino, A. J. van Wijnen, G. S. Stein, J. L. Stein, and J. B. Lian
Dlx3 Transcriptional Regulation of Osteoblast Differentiation: Temporal Recruitment of Msx2, Dlx3, and Dlx5 Homeodomain Proteins to Chromatin of the Osteocalcin Gene
Mol. Cell. Biol., October 15, 2004; 24(20): 9248 - 9261.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Thomas, P. Moffatt, P. Salois, M.-H. Gaumond, R. Gingras, E. Godin, D. Miao, D. Goltzman, and C. Lanctot
Osteocrin, a Novel Bone-specific Secreted Protein That Modulates the Osteoblast Phenotype
J. Biol. Chem., December 12, 2003; 278(50): 50563 - 50571.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. D. Devlin, Z. Du, R. C. Pereira, R. B. Kimble, A. N. Economides, V. Jorgetti, and E. Canalis
Skeletal Overexpression of Noggin Results in Osteopenia and Reduced Bone Formation
Endocrinology, May 1, 2003; 144(5): 1972 - 1978.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. D. Devlin, Z. Du, V. Buccilli, V. Jorgetti, and E. Canalis
Transgenic Mice Overexpressing Insulin-Like Growth Factor Binding Protein-5 Display Transiently Decreased Osteoblastic Function and Osteopenia
Endocrinology, October 1, 2002; 143(10): 3955 - 3962.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H. Drissi, D. Hushka, F. Aslam, Q. Nguyen, E. Buffone, A. Koff, A. J. van Wijnen, J. B. Lian, J. L. Stein, and G. S. Stein
The Cell Cycle Regulator p27kip1 Contributes to Growth and Differentiation of Osteoblasts
Cancer Res., August 1, 1999; 59(15): 3705 - 3711.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Hou, Q. Nguyen, B. Frenkel, S. K. Nilsson, M. Milne, A. J. van Wijnen, J. L. Stein, P. Quesenberry, J. B. Lian, and G. S. Stein
Osteoblast-specific gene expression after transplantation of marrow cells: Implications for skeletal gene therapy
PNAS, June 22, 1999; 96(13): 7294 - 7299.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E Filvaroff, A Erlebacher, J Ye, S. Gitelman, J Lotz, M Heillman, and R Derynck
Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass
Development, January 10, 1999; 126(19): 4267 - 4279.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J.-L. Frendo, G. Xiao, S. Fuchs, R. T. Franceschi, G. Karsenty, and P. Ducy
Functional Hierarchy between Two OSE2 Elements in the Control of Osteocalcin Gene Expression in Vivo
J. Biol. Chem., November 13, 1998; 273(46): 30509 - 30516.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B.-Y. Liu, J. Guo, B. Lanske, P. Divieti, H. M. Kronenberg, and F. R. Bringhurst
Conditionally Immortalized Murine Bone Marrow Stromal Cells Mediate Parathyroid Hormone-Dependent Osteoclastogenesis in Vitro
Endocrinology, April 1, 1998; 139(4): 1952 - 1964.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. O. Carpenter, K. C. Moltz, B. Ellis, M. Andreoli, T. L. McCarthy, M. Centrella, D. Bryan, and C. M. Gundberg
Osteocalcin Production in Primary Osteoblast Cultures Derived from Normal and Hyp Mice
Endocrinology, January 1, 1998; 139(1): 35 - 43.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
N. A. Sims, C. P. White, K. L. Sunn, G. P. Thomas, M. L. Drummond, N. A. Morrison, J. A. Eisman, and E. M. Gardiner
Human and Murine Osteocalcin Gene Expression: Conserved Tissue Restricted Expression and Divergent Responses to 1,25-Dihydroxyvitamin D3 in Vivo
Mol. Endocrinol., October 1, 1997; 11(11): 1695 - 1708.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society