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Expression of Cyclooxygenase-1 and -2 in Ovine Endometrium During the Estrous Cycle and Early Pregnancy¹

Laboratoire de Physiologie Animale, Institut National de la Recherche Agronomique (G.C., P.R., J-P.T., J.Mar.), 78352 Jouy-en-Josas Cedex; Commissariat à l’Energie Atomique, Service de Pharmacologie et d’Immunologie, Centre d’Etudes Saclay (C.C.), 91191 Gif-sur-Yvette Cedex; I.F.R. Biologie de la Circulation-Lariboisière, INSERM U 348, Hôpital Lariboisière (J.Mac.), 75475 Paris Cedex 10; and Unité Recherche Associée CNRS 1291, laboratoire de Physiologie Animale, Institut National de la Recherche Agronomique (M.G.), 78352 Jouy-en-Josas Cedex, France

Address all correspondence and requests for reprints to: G. Charpigny; laboratoire de Physiologie Animale, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas cedex, France. E-mail: gch{at}jouy.inra.fr

In this study we investigated the expression of the two cyclooxygenases, cox-1 and -2, in sheep uterine tissues during the estrous cycle and early pregnancy. We identified the cox-2 isoform in the ovine uterus by Western blot and demonstrated that the two cyclooxygenases exhibited different patterns of expression. Cox-1 was expressed at steady state levels in the endometrium during the estrous cycle and comparable stages of pregnancy. In contrast, cox-2 was highly and transiently expressed from days 12–15 of the estrous cycle and declined thereafter to undetectable levels. Endometrium from early pregnant ewes showed a similar pattern of cox-2 expression, although there was a slower decrease beyond day 15. Immunohistochemical studies demonstrated that cox-1 was localized in both epithelial and stromal cells, whereas cox-2 was localized solely in the luminal epithelium and to a lesser extent in the superficial glands. Treatment of ovariectomized ewes with steroids indicated that expression of cox-1 remained at constant levels whatever the treatment. In contrast, endometrial cox-2 was highly induced by a 10-day progesterone treatment. Estradiol slightly increased cox-2 expression but only after progesterone priming. Collectively these results suggest that the developing ability of the uterus to synthesize PGs is due to the induction of cox-2.

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