help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Upton, P. D.
Right arrow Articles by Smith, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Upton, P. D.
Right arrow Articles by Smith, D. M.
Endocrinology Vol. 138, No. 6 2508-2514
Copyright © 1997 by The Endocrine Society

ARTICLES

Expression of Adrenomedullin (ADM) and Its Binding Sites in the Rat Uterus: Increased Number of Binding Sites and ADM Messenger Ribonucleic Acid in 20-Day Pregnant Rats Compared with Nonpregnant Rats1

Paul D. Upton, Carol Austin, Gillian M. Taylor2, Kiran A. Nandha, Adrian J. L. Clark, Mohammad A. Ghatei, Stephen R. Bloom and David M. Smith

Division of Endocrinology and Metabolic Medicine (P.D.U., C.A., G.M.T., K.A.N., M.A.G., S.R.B., D.M.S.), Royal Postgraduate Medical School, Hammersmith Hospital, London W12 ONN, United Kingdom; and Department of Clinical Endocrinology (A.J.L.C.), St. Bartholomew’s Hospital Medical College, London, EC1A 7BE, United Kingdom

Address all correspondence and requests for reprints to: D. M. Smith, Ph.D., Division of Endocrinology and Metabolic Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 ONN, United Kingdom. E-mail: dsmith{at}rpms.ac.uk

RIA of nonpregnant rat uterus extracts showed 0.68 ± 0.08 pmol/g adrenomedullin (ADM) and 3.23 ± 0.08 pmol/g calcitonin gene-related peptide (CGRP). In the pregnant (20 days gestation) uterus, the ADM content was 0.90 ± 0.17 pmol/g, and CGRP could not be detected. ADM messenger RNA was detected at high levels in the uterus, with a 1.8-fold increase in expression in pregnancy. Pharmacologically distinct binding sites for ADM (Bmax = 21 ± 2 fmol/mg protein, dissociation constant = 80 ± 6 pM), and CGRP (Bmax = 101 ± 18 fmol/mg protein, dissociation constant = 140 ± 20 pM) were identified in nonpregnant uterus. Competition for 125I[Tyr0]{alpha}CGRP binding was shown by both ADM and CGRP (8–37), whereas CGRP and CGRP (8–37) did not compete for 125I-ADM-binding sites. The density of the ADM-binding sites was 10 times greater in pregnant uterus (Bmax = 211 ± 39 fmol/mg protein, P < 0.01) than nonpregnant uterus. CGRP receptor messenger RNA was identified in both nonpregnant and pregnant uteri. In isolated nonpregnant rat uteri, CGRP and ADM attenuated the contractile response to galanin by 77 ± 10% and 57 ± 10%, respectively. The responses to both CGRP and ADM were abolished by CGRP (8–37). These results demonstrate, for the first time, the presence of ADM and specific binding sites for both ADM and CGRP in the rat uterus.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
Y.-F. Chan, W.-S. O, and F. Tang
Adrenomedullin in the Rat Testis. I: Its Production, Actions on Testosterone Secretion, Regulation by Human Chorionic Gonadotropin, and Its Interaction with Endothelin 1 in the Leydig Cell
Biol Reprod, April 1, 2008; 78(4): 773 - 779.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
Y.-Y. Li, W.-S. O, and F. Tang
Effect of Aging on the Expression of Adrenomedullin and Its Receptor Component Proteins in the Male Reproductive System of the Rat
J. Gerontol. A Biol. Sci. Med. Sci., December 1, 2007; 62(12): 1346 - 1351.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
E. Marinoni, C. Zacharopoulou, A. Di Rocco, C. Letizia, M. Moscarini, and R. Di Iorio
Effect of Betamethasone In Vivo on Placental Adrenomedullin in Human Pregnancy
Reproductive Sciences, September 1, 2006; 13(6): 418 - 424.
[Abstract] [PDF]

Home page
 Biol. Reprod.Home page
Y.-Y. Li, I. S.-S. Hwang, W.-S. O, and F. Tang
Adrenomedullin Peptide: Gene Expression of Adrenomedullin, its Receptors and Receptor Activity Modifying Proteins, and Receptor Binding in Rat Testis--Actions on Testosterone Secretion
Biol Reprod, August 1, 2006; 75(2): 183 - 188.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
H Watanabe, E Takahashi, M Kobayashi, M Goto, A Krust, P Chambon, and T Iguchi
The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor
J. Mol. Endocrinol., February 1, 2006; 36(1): 81 - 89.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Thota and C. Yallampalli
Progesterone Upregulates Calcitonin Gene-Related Peptide and Adrenomedullin Receptor Components and Cyclic Adenosine 3'5'-Monophosphate Generation in Eker Rat Uterine Smooth Muscle Cell Line
Biol Reprod, February 1, 2005; 72(2): 416 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Thota, P.R.R. Gangula, Y.L. Dong, and C. Yallampalli
Changes in the Expression of Calcitonin Receptor-Like Receptor, Receptor Activity-Modifying Protein (RAMP) 1, RAMP2, and RAMP3 in Rat Uterus During Pregnancy, Labor, and by Steroid Hormone Treatments
Biol Reprod, October 1, 2003; 69(4): 1432 - 1437.
[Abstract] [Full Text] [PDF]

Home page
 Obstet GynecolHome page
M. Nakatsuka, T. Habara, S. Noguchi, H. Konishi, and T. Kudo
Increased Plasma Adrenomedullin in Women With Recurrent Pregnancy Loss
Obstet. Gynecol., August 1, 2003; 102(2): 319 - 324.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
I. S.S. Hwang, D. J. Autelitano, P. Y.D. Wong, G. P.H. Leung, and F. Tang
Co-Expression of Adrenomedullin and Adrenomedullin Receptors in Rat Epididymis: Distinct Physiological Actions on Anion Transport
Biol Reprod, June 1, 2003; 68(6): 2005 - 2012.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J.B. Laoag-Fernandez, T. Maruo, P. Pakarinen, I.M. Spitz, and E. Johansson
Effects of levonorgestrel-releasing intra-uterine system on the expression of vascular endothelial growth factor and adrenomedullin in the endometrium in adenomyosis
Hum. Reprod., April 1, 2003; 18(4): 694 - 699.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
D. R. Poyner, P. M. Sexton, I. Marshall, D. M. Smith, R. Quirion, W. Born, R. Muff, J. A. Fischer, and S. M. Foord
International Union of Pharmacology. XXXII. The Mammalian Calcitonin Gene-Related Peptides, Adrenomedullin, Amylin, and Calcitonin Receptors
Pharmacol. Rev., June 1, 2002; 54(2): 233 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
V. A. Cameron, D. J. Autelitano, J. J. Evans, L. J. Ellmers, E. A. Espiner, M. G. Nicholls, and A. M. Richards
Adrenomedullin expression in rat uterus is correlated with plasma estradiol
Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E139 - E146.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
L.L. Nikitenko, N.S. Brown, D.M. Smith, I.Z. MacKenzie, R. Bicknell, and M.C.P. Rees
Differential and cell-specific expression of calcitonin receptor-like receptor and receptor activity modifying proteins in the human uterus
Mol. Hum. Reprod., July 1, 2001; 7(7): 655 - 664.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
L.L. Nikitenko, I.Z. MacKenzie, M.C.P. Rees, and R. Bicknell
Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium
Mol. Hum. Reprod., September 1, 2000; 6(9): 811 - 819.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. P. Hinson, S. Kapas, and D. M. Smith
Adrenomedullin, a Multifunctional Regulatory Peptide
Endocr. Rev., April 1, 2000; 21(2): 138 - 167.
[Abstract] [Full Text]

Home page
 IOVSHome page
S. Y. K. Yousufzai, N. Ali, and A. A. Abdel-Latif
Effects of Adrenomedullin on Cyclic AMP Formation and on Relaxation in Iris Sphincter Smooth Muscle
Invest. Ophthalmol. Vis. Sci., December 1, 1999; 40(13): 3245 - 3253.
[Abstract] [Full Text] [PDF]

Home page
 Obstet GynecolHome page
E. MARINONI, R. DI IORIO, C. LETIZIA, B. VILLACCIO, A. ALBERINI, and E. V. COSMI
Amniotic Fluid Concentrations of Adrenomedullin in Preterm Labor
Obstet. Gynecol., June 1, 1999; 93(6): 964 - 967.
[Abstract] [Full Text] [PDF]

Home page
 Obstet GynecolHome page
M. MICHISHITA, T. MINEGISHI, K. ABE, K. KANGAWA, M. KOJIMA, and Y. IBUKI
Expression of Adrenomedullin in the Endometrium of the Human Uterus
Obstet. Gynecol., January 1, 1999; 93(1): 66 - 70.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
R. Di Iorio, E. Marinoni, C. Letizia, P. Alo, B. Villaccio, and E. V. Cosmi
Adrenomedullin, a New Vasoactive Peptide, Is Increased in Preeclampsia
Hypertension, October 1, 1998; 32(4): 758 - 763.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. A. Cameron and A. M. Fleming
Novel Sites of Adrenomedullin Gene Expression in Mouse and Rat Tissues
Endocrinology, May 1, 1998; 139(5): 2253 - 2264.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ladoux and C. Frelin
Coordinated Up-regulation by Hypoxia of Adrenomedullin and One of Its Putative Receptors (RDC-1) in Cells of the Rat Blood-Brain Barrier
J. Biol. Chem., December 15, 2000; 275(51): 39914 - 39919.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society