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Interleukin (IL)-1ß Increases Glucose Uptake and Induces Glycolysis in Aerobically Cultured Rat Ovarian Cells: Evidence That IL-1ß May Mediate the Gonadotropin-Induced Midcycle Metabolic Shift¹

Division of Reproductive Endocrinology (I.B.-S., S.K., C.E.R., A.H. D.W.P., E.Y.A.), Department of Obstetrics and Gynecology, and Division of Pediatric Research (L.M.R.), Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland 21201

Address all correspondence and requests for reprints to: Dr. Eli Y. Adashi or Dr. Donna W. Payne, The University of Utah, Department of Obstetrics and Gynecology, Health Sciences Center, Suite 2B200, 50 North Medical Drive, Salt Lake City, Utah 84132. E-mail: eadashi{at}hsc.utah.edu

This communication explores the possibility that interleukin (IL)-1ß, a putative intermediary in the ovulatory process, may take part in the gonadotropin-driven midcycle diversion of ovarian carbohydrate metabolism toward glycolysis. We examined the effect of treatment with IL-1ß on glucose metabolism in aerobically cultured whole ovarian dispersates from immature rats. Treatment with IL-1ß increased cellular glucose consumption/uptake, stimulated extracellular lactate accumulation and media acidification, and decreased extracellular pyruvate accumulation in a receptor-mediated, time-, dose- and cell density-dependent manner. Endogenous IL-1ß-like bioactivity was shown to mediate the ability of gonadotropins to exert these same metabolic effects. The IL-1ß effect was also (1) apparent over a broad range of glucose concentrations, inclusive of the putative physiological window; (2) relatively specific, because tumor necrosis factor- and insulin were inactive; (3) contingent upon cell-cell cooperation (4) and reliant on de novo protein synthesis. Comparison of the molar ratios of lactate accumulation to glucose consumption in IL-1ß-replete vs. IL-1ß-deplete cultures suggests that IL-ß promotes the conversion of all available glucose to lactate but that other substrates for lactate production may also exist. However, no lactate was generated by cells grown under glucose-free conditions. Taken together, our data suggest that IL-1ß may act as a metabolic hormone in the ovary. Subject to the limitations of the in vitro paradigm, our data also suggest that IL-1ß may mediate the gonadotropin-associated midcycle shift in ovarian carbohydrate metabolism. By converting the somatic ovarian cells into a glucose-consuming glycolytic machinery, IL-1ß may establish glycolysis as the main energy source of the relatively hypoxic preovulatory follicle and the resultant cumulus-oocyte complex. The consequent oxygen sparing may conserve the limited supply of oxygen needed for vital biosynthetic processes such as steroidogenesis. This adaptational response may also provide the glycolytically incompetent oocyte with the obligatory tricarboxylic cycle precursors it depends on to meet the increased energy demands imposed upon it by the resumption of meiosis.

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