help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Akinola, L. A.
Right arrow Articles by Vihko, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Akinola, L. A.
Right arrow Articles by Vihko, P.
Endocrinology Vol. 138, No. 7 2886-2892
Copyright © 1997 by The Endocrine Society

ARTICLES

Expression of 17ß-Hydroxysteroid Dehydrogenase Type 1 and Type 2, P450 Aromatase, and 20{alpha}-Hydroxysteroid Dehydrogenase Enzymes in Immature, Mature, and Pregnant Rats1

Lateef A. Akinola2, Matti Poutanen, Reijo Vihko and Pirkko Vihko

Biocenter Oulu and the Department of Clinical Chemistry, University of Oulu, FIN-90220 Oulu, Finland

Address all correspondence and requests for reprints to: Prof. Pirkko Vihko, Biocenter Oulu and Department of Clinical Chemistry, University of Oulu, FIN-90220 Oulu, Finland.

In the present study, we evaluated the expression and regulation of 17ß-hydroxysteroid dehydrogenase (17HSD) type 1 and type 2, cytochrome P450 aromatase (P450arom), and 20{alpha}-hydroxysteroid dehydrogenase (20HSD) in mature and pregnant rats. Immunohistochemical analysis of rat 17HSD type 1 showed that the enzyme is exclusively expressed in the granulosa cells of developing, healthy, primary, secondary, and tertiary follicles at all stages of the estrous cycle and pregnancy, and is not detected in the corpora lutea. The data showed that the amount of the enzyme expressed in the follicle increases as follicular maturation progresses and is highest in tertiary and Graafian follicles. However, Northern blot analysis of total RNA from whole ovaries showed a rather constitutive expression of the 17HSD type 1 enzyme. It is evident that compared with P450arom, 17HSD type 1 is more widely expressed in the follicles during the various maturational stages of folliculogenesis. Hence, the data indicate distinct localization, expression, and regulation patterns for 17HSD type 1 and P450arom during the rat estrous cycle and pregnancy. Furthermore, compared with the two estradiol biosynthetic enzymes, a different expression pattern was detected for 20HSD messenger RNA. During the estrous cycle the enzyme was detected in the ovaries throughout the cycle, and in the ovaries of pregnant animals the enzyme showed an expression pattern the opposite of that observed for P450arom. Rat 17HSD type 2, not detected in the ovaries, was constitutively expressed in both female and male liver and small intestine in 21-day-old fetuses up to 6-week-old mature animals. Similarly, in these tissues the enzyme was constitutively expressed in normal cycling and pregnant animals, but it showed increasing expression in the placenta as pregnancy advanced. The relatively constitutive expression of the enzyme at all physiological stages of the animals suggests a general role for the enzyme in the inactivation of circulating sex steroids.




This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
C. Stocco, J. Kwintkiewicz, and Z. Cai
Identification of regulatory elements in the Cyp19 proximal promoter in rat luteal cells
J. Mol. Endocrinol., October 1, 2007; 39(4): 211 - 221.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Zhongyi, P. Rantakari, T. Lamminen, J. Toppari, and M. Poutanen
Transgenic Male Mice Expressing Human Hydroxysteroid Dehydrogenase 2 Indicate a Role for the Enzyme Independent of Its Action on Sex Steroids
Endocrinology, August 1, 2007; 148(8): 3827 - 3836.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Hershkovitz, F. Beuschlein, S. Klammer, M. Krup, and Y. Weinstein
Adrenal 20{alpha}-Hydroxysteroid Dehydrogenase in the Mouse Catabolizes Progesterone and 11-Deoxycorticosterone and Is Restricted to the X-Zone
Endocrinology, March 1, 2007; 148(3): 976 - 988.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
K. A Brown, K. Sayasith, N. Bouchard, J. G Lussier, and J. Sirois
Molecular cloning of equine 17{beta}-hydroxysteroid dehydrogenase type 1 and its downregulation during follicular luteinization in vivo
J. Mol. Endocrinol., January 1, 2007; 38(1): 67 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Foyouzi, Z. Cai, Y. Sugimoto, and C. Stocco
Changes in the Expression of Steroidogenic and Antioxidant Genes in the Mouse Corpus Luteum During Luteolysis
Biol Reprod, May 1, 2005; 72(5): 1134 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. P. Piekorz, S. Gingras, A. Hoffmeyer, J. N. Ihle, and Y. Weinstein
Regulation of Progesterone Levels during Pregnancy and Parturition by Signal Transducer and Activator of Transcription 5 and 20{alpha}-Hydroxysteroid Dehydrogenase
Mol. Endocrinol., February 1, 2005; 19(2): 431 - 440.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Stocco
In Vivo and in Vitro Inhibition of cyp19 Gene Expression by Prostaglandin F2{alpha} in Murine Luteal Cells: Implication of GATA-4
Endocrinology, November 1, 2004; 145(11): 4957 - 4966.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
G Pelletier, V Luu-The, S Li, L Ren, and F Labrie
Localization of 17{beta}-hydroxysteroid dehydrogenase type 1 mRNA in mouse tissues
J. Mol. Endocrinol., October 1, 2004; 33(2): 459 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
V. D. Doan, S. Gagnon, and V. Joseph
Prenatal blockade of estradiol synthesis impairs respiratory and metabolic responses to hypoxia in newborn and adult rats
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R612 - R618.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B. Husen, J. Adamski, A. Bruns, D. Deluca, K. Fuhrmann, G. Moller, I. Schwabe, and A. Einspanier
Characterization of 17{beta}-Hydroxysteroid Dehydrogenase Type 7 in Reproductive Tissues of the Marmoset Monkey
Biol Reprod, June 1, 2003; 68(6): 2092 - 2099.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. C. J. van der Eerden, J. van de Ven, C. W. G. M. Lowik, J. M. Wit, and M. Karperien
Sex Steroid Metabolism in the Tibial Growth Plate of the Rat
Endocrinology, October 1, 2002; 143(10): 4048 - 4055.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Wang, K. Andoh, H. Hagiwara, L. Xiaowei, N. Kikuchi, Y. Abe, K. Yamada, R. Fatima, and H. Mizunuma
Effect of Adrenal and Ovarian Androgens on Type 4 Follicles Unresponsive to FSH in Immature Mice
Endocrinology, November 1, 2001; 142(11): 4930 - 4936.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Nokelainen, H. Peltoketo, M. Mustonen, and P. Vihko
Expression of Mouse 17{beta}-Hydroxysteroid Dehydrogenase/17-Ketosteroid Reductase Type 7 in the Ovary, Uterus, and Placenta: Localization from Implantation to Late Pregnancy
Endocrinology, February 1, 2000; 141(2): 772 - 778.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. S. Mahendroo, A. Porter, D. W. Russell, and R. A. Word
The Parturition Defect in Steroid 5{alpha}-Reductase Type 1 Knockout Mice Is Due to Impaired Cervical Ripening
Mol. Endocrinol., June 1, 1999; 13(6): 981 - 992.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
P. Nokelainen, H. Peltoketo, R. Vihko, and P. Vihko
Expression Cloning of a Novel Estrogenic Mouse 17{beta}-Hydroxysteroid Dehydrogenase/ 17-Ketosteroid Reductase (m17HSD7), Previously Described as a Prolactin Receptor-Associated Protein (PRAP) in Rat
Mol. Endocrinol., July 1, 1998; 12(7): 1048 - 1059.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society