help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Veldman, C. M.
Right arrow Articles by Schmid, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Veldman, C. M.
Right arrow Articles by Schmid, C.
Endocrinology Vol. 139, No. 1 89-94
Copyright © 1998 by The Endocrine Society

ARTICLES

Prostaglandin E2 Stimulates Sodium-Dependent Phosphate Transport in Osteoblastic Cells via a Protein Kinase C-Mediated Pathway1

Christian M. Veldman, Irene Schläpfer and Christoph Schmid

Division of Endocrinology and Metabolism, Department of Internal Medicine, University Hospital, CH-8091 Zurich, Switzerland

Address all correspondence and requests for reprints to: Dr. Christoph Schmid, Division of Endocrinology and Diabetes, Department of Internal Medicine, University Hospital, CH-8091 Zurich, Switzerland.

Eicosanoids are released by bone cells in response to physical, hormonal, and cytokine stimulation, and they can both inhibit and stimulate bone formation. We investigated the effect of PGE2 on sodium-dependent phosphate (NadPi) transport in a rat bone-derived cell line, PyMS. PGE2 and 12-O-tetradecanoyl phorbol-13-acetate (TPA), an activator of protein kinase C, increased NadPi uptake in a dose- and time-dependent manner. There was no change in Na-dependent alanine transport, and the effects of PGE2 and TPA were not associated with corresponding changes in cell number or protein content. Their effects on NadPi uptake were not additive. Calphostin C, an inhibitor of protein kinase C (10-8 M) completely blocked TPA- and PGE2-stimulated NadPi uptake. The results are consistent with a crucial role of protein kinase C activation in the short term stimulation of NadPi transport by PGE2 in PyMS cells.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
K. A. Abraham, J. J. Brault, and R. L. Terjung
Phosphate uptake and PiT-1 protein expression in rat skeletal muscle
Am J Physiol Cell Physiol, July 1, 2004; 287(1): C73 - C78.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. R. Beck Jr. and N. Knecht
Osteopontin Regulation by Inorganic Phosphate Is ERK1/2-, Protein Kinase C-, and Proteasome-dependent
J. Biol. Chem., October 24, 2003; 278(43): 41921 - 41929.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Yoshiko, N. Maeda, and J. E. Aubin
Stanniocalcin 1 Stimulates Osteoblast Differentiation in Rat Calvaria Cell Cultures
Endocrinology, September 1, 2003; 144(9): 4134 - 4143.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Jobbagy, Z. Olah, G. Petrovics, M. V. Eiden, B. D. Leverett, N. M. Dean, and W. B. Anderson
Up-regulation of the Pit-2 Phosphate Transporter/Retrovirus Receptor by Protein Kinase C epsilon
J. Biol. Chem., March 12, 1999; 274(11): 7067 - 7071.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society