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The V_1a and V_1b, But Not V₂, Vasopressin Receptor Genes Are Expressed in the Supraoptic Nucleus of the Rat Hypothalamus, and the Transcripts Are Essentially Colocalized in the Vasopressinergic Magnocellular Neurons

Centre National de la Recherche Scientifique, Unité Propre de Recherche 9055, Biologie des Neurones Endocrines, Centre de Pharmacologie-Endocrinologie, F-34094 Montpellier Cedex 5, France

Address all correspondence and requests for reprints to: Dr. F. Moos, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9055, Centre de Pharmacologie-Endocrinologie, 141 rue de la Cardonille, F-34094 Montpellier Cedex 5, France. E-mail: moos{at}ccipe.montp.inserm.fr

We have identified and visualized the vasopressin (VP) receptors expressed by hypothalamic magnocellular neurons in supraoptic and paraventricular nuclei. To do this, we used RT-PCR on total RNA extracts from supraoptic nuclei or on single freshly dissociated supraoptic neurons, and in situ hybridization on frontal sections of hypothalamus of Wistar rats.

The RT-PCR on supraoptic RNA extracts revealed that mainly V_1a, but also V_1b, subtypes of VP receptors are expressed from birth to adulthood. No V₂ receptor messenger RNA (mRNA) was detected. Furthermore, the single-cell RT-nested PCR indicated that the V_1a receptor mRNA is present in vasopressinergic magnocellular neurons.

In light of these results, in situ hybridization was performed to visualize the V_1a and V_1b receptor mRNAs in supraoptic and paraventricular nuclei. Simultaneously, we coupled this approach to: 1) in situ hybridization detection of oxytocin or VP mRNAs; or 2) immunocytochemistry to detect the neuropeptides. This provided a way of identifying the neurons expressing perceptible amounts of V_1a or V_1b receptor mRNAs as vasopressinergic neurons.

Here, we suggest that the autocontrol exerted specifically by VP on vasopressinergic neurons is mediated through, at least, V_1a and V_1b subtype receptors.

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