| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ARTICLES |
Département de Physiologie, Faculté de Médecine, Université Laval, Québec G1K 7P4, Canada
Address all correspondence and requests for reprints to: Denis Richard, Département de Physiologie, Faculté de Médecine, Université Laval, Québec G1K 7P4, Canada. E-mail: denis.richard{at}phs.ulaval.ca
The effects of leptin on the levels of CRF messenger RNA (mRNA) in the paraventricular hypothalamic nucleus (PVN), on the activation of the PVN CRF cells, and on the plasma levels of corticosterone were investigated in lean (+/?) and obese (ob/ob) C57BL/6J male mice. Murine leptin was sc infused using osmotic minipumps. The treatment period extended to 7 days, and the daily dose of leptin delivered was 100 µg/kg. The mice were killed either in a fed state or following 24 h of total food deprivation. The starvation paradigm was employed to enhance the activity of the hypothalamic-pituitary-adrenal axis in obese mice. In situ hybridization histochemistry was performed to determine the PVN levels of CRF mRNA and the arcuate nucleus levels of neuropeptide Y mRNA. The activity of the PVN CRF cells was estimated from the number of PVN cells colocalizing CRF mRNA and the protein Fos. Leptin led to a reduction in body weight gain and fat deposition. These effects were seen in both +/? and ob/ob mice and were observed to be particularly striking in obese mutants, in which leptin also caused an important reduction in food intake. Leptin also was found to affect plasma levels of corticosterone. It lowered the high corticosterone levels of obese mutants, an effect that appeared more evident in food-deprived than in fed mice. Finally, leptin prevented the induction of CRF synthesis in the PVN and the activation of the PVN CRF neurons observed in food-deprived ob/ob mice and hindered the elevation of arcuate nucleus neuropeptide Y synthesis in ob/ob mice. Together these results suggest a role for leptin in the excessive response of the hypophysiotropic CRF system of the ob/ob mouse.
This article has been cited by other articles:
 |
|
 |
|
  D. L. Morris and L. Rui Recent advances in understanding leptin signaling and leptin resistance Am J Physiol Endocrinol Metab, December 1, 2009; 297(6): E1247 - E1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Luque, Z. H. Huang, B. Shah, T. Mazzone, and R. D. Kineman Effects of leptin replacement on hypothalamic-pituitary growth hormone axis function and circulating ghrelin levels in ob/ob mice Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E891 - E899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Luque, S. Park, and R. D. Kineman Severity of the Catabolic Condition Differentially Modulates Hypothalamic Expression of Growth Hormone-Releasing Hormone in the Fasted Mouse: Potential Role of Neuropeptide Y and Corticotropin-Releasing Hormone Endocrinology, January 1, 2007; 148(1): 300 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Huang, E. Timofeeva, and D. Richard Regulation of corticotropin-releasing factor and its types 1 and 2 receptors by leptin in rats subjected to treadmill running-induced stress. J. Endocrinol., October 1, 2006; 191(1): 179 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. H. Jethwa, K. L. Smith, C. J. Small, C. R. Abbott, S. J. Darch, K. G. Murphy, A. Seth, N. M. Semjonous, S. R. Patel, J. F. Todd, et al. Neuromedin U Partially Mediates Leptin-Induced Hypothalamo-Pituitary Adrenal (HPA) Stimulation and Has a Physiological Role in the Regulation of the HPA Axis in the Rat Endocrinology, June 1, 2006; 147(6): 2886 - 2892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sahu Minireview: A Hypothalamic Role in Energy Balance with Special Emphasis on Leptin Endocrinology, June 1, 2004; 145(6): 2613 - 2620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Salzmann, M. Otis, H. Long, C. Roberge, N. Gallo-Payet, and C.-D. Walker Inhibition of Steroidogenic Response to Adrenocorticotropin by Leptin: Implications for the Adrenal Response to Maternal Separation in Neonatal Rats Endocrinology, April 1, 2004; 145(4): 1810 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Liao, M.-B. Lee, Y.-J. Lee, and T.-S. Huang Hyperleptinemia in Subjects With Persistent Partial Posttraumatic Stress Disorder After a Major Earthquake Psychosom Med, January 1, 2004; 66(1): 23 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Masaki, G. Yoshimichi, S. Chiba, T. Yasuda, H. Noguchi, T. Kakuma, T. Sakata, and H. Yoshimatsu Corticotropin-Releasing Hormone-Mediated Pathway of Leptin to Regulate Feeding, Adiposity, and Uncoupling Protein Expression in Mice Endocrinology, August 1, 2003; 144(8): 3547 - 3554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Masaki, S. Chiba, G. Yoshimichi, T. Yasuda, H. Noguchi, T. Kakuma, T. Sakata, and H. Yoshimatsu Neuronal Histamine Regulates Food Intake, Adiposity, and Uncoupling Protein Expression in Agouti Yellow (Ay/a) Obese Mice Endocrinology, June 1, 2003; 144(6): 2741 - 2748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Proulx, D. Richard, and C.-D. Walker Leptin Regulates Appetite-Related Neuropeptides in the Hypothalamus of Developing Rats without Affecting Food Intake Endocrinology, December 1, 2002; 143(12): 4683 - 4692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Okimoto, A. Blaus, M. Schmidt, M. K. Gordon, G. W. Dent, and S. Levine Differential Expression of c-fos and Tyrosine Hydroxylase mRNA in the Adrenal Gland of the Infant Rat: Evidence for an Adrenal Hyporesponsive Period Endocrinology, May 1, 2002; 143(5): 1717 - 1725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. J. Ellacott, C. B. Lawrence, N. J. Rothwell, and S. M. Luckman PRL-Releasing Peptide Interacts with Leptin to Reduce Food Intake and Body Weight Endocrinology, February 1, 2002; 143(2): 368 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Proulx, S. Clavel, G. Nault, D. Richard, and C.-D. Walker High Neonatal Leptin Exposure Enhances Brain GR Expression and Feedback Efficacy on the Adrenocortical Axis of Developing Rats Endocrinology, November 1, 2001; 142(11): 4607 - 4616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lacroix, N. N'Diaye, J. Tremblay, and P. Hamet Ectopic and Abnormal Hormone Receptors in Adrenal Cushing's Syndrome Endocr. Rev., February 1, 2001; 22(1): 75 - 110. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Masaki, H. Yoshimatsu, S. Chiba, T. Watanabe, and T. Sakata Central Infusion of Histamine Reduces Fat Accumulation and Upregulates UCP Family in Leptin-Resistant Obese Mice Diabetes, February 1, 2001; 50(2): 376 - 384. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. E. Levin, D. Richard, C. Michel, and R. Servatius Differential stress responsivity in diet-induced obese and resistant rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2000; 279(4): R1357 - R1364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vidal, S. M. Cohen, E. Horvath, K. Kovacs, B. W. Scheithauer, B. G. Burguera, and R. V. Lloyd Subcellular Localization of Leptin in Non-tumorous and Adenomatous Human Pituitaries: An Immuno-ultrastructural Study J. Histochem. Cytochem., August 1, 2000; 48(8): 1147 - 1152. [Abstract] [Full Text]
|
|
|
|
|
|
V. A. Boundy and A. H. Cincotta Hypothalamic adrenergic receptor changes in the metabolic syndrome of genetically obese (ob/ob) mice Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2000; 279(2): R505 - R514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Timofeeva, Y. Deshaies, F. Picard, and D. Richard Corticotropin-releasing hormone-binding protein in brain and pituitary of food-deprived obese (fa/fa) Zucker rats Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1999; 277(6): R1749 - R1759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Ahima, J. Kelly, J. K. Elmquist, and J. S. Flier Distinct Physiologic and Neuronal Responses to Decreased Leptin and Mild Hyperleptinemia Endocrinology, November 1, 1999; 140(11): 4923 - 4931. [Abstract] [Full Text]
|
|
|
|
|
|
G. van Dijk, R. J. Seeley, T. E. Thiele, M. I. Friedman, H. Ji, C. W. Wilkinson, P. Burn, L. A. Campfield, R. Tenenbaum, D. G. Baskin, et al. Metabolic, gastrointestinal, and CNS neuropeptide effects of brain leptin administration in the rat Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1999; 276(5): R1425 - R1433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Silver, X.-c. Jiang, and A. R. Tall Increased High Density Lipoprotein (HDL), Defective Hepatic Catabolism of ApoA-I and ApoA-II, and Decreased ApoA-I mRNA in ob/ob Mice. POSSIBLE ROLE OF LEPTIN IN STIMULATION OF HDL TURNOVER J. Biol. Chem., February 12, 1999; 274(7): 4140 - 4146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Trottier, K. G. Koski, T. Brun, D. J. Toufexis, D. Richard, and C.-D. Walker Increased Fat Intake during Lactation Modifies Hypothalamic-Pituitary-Adrenal Responsiveness in Developing Rat Pups: A Possible Role for Leptin Endocrinology, September 1, 1998; 139(9): 3704 - 3711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Arvaniti, Y. Deshaies, and D. Richard Effect of leptin on energy balance does not require the presence of intact adrenals Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1998; 275(1): R105 - R111. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
