help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kimura, N.
Right arrow Articles by Kimura, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kimura, N.
Right arrow Articles by Kimura, N.
Endocrinology Vol. 139, No. 4 1573-1580
Copyright © 1998 by The Endocrine Society

ARTICLES

Chronic Treatment with Estrogen Up-Regulates Expression of sst2 Messenger Ribonucleic Acid (mRNA) but Down-Regulates Expression of sst5 mRNA in Rat Pituitaries1

Nobuko Kimura, Sanae Tomizawa, Kazuko Nakata Arai and Narimichi Kimura

Department of Molecular Neurobiology (No.K., S.T., K.N.A.), Tokyo Metropolitan Institute for Neuroscience, Fuchu-shi, Tokyo 183; and the Department of Molecular Biology (Na.K.), Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173, Japan

Address all correspondence and requests for reprints to: Dr. Nobuko Kimura, Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, 2–6 Musashidai, Fuchu-shi, Tokyo-183, Japan. E-mail: kimura{at}tmin.ac.jp

We previously showed that 17ß-estradiol (E2) induces the somatostatin (SRIF) responsiveness of the rat anterior pituitary, which leads to inhibition of PRL secretion through E2-dependent SRIF receptors. To examine receptor subtypes regulated by E2, we determined the messenger ribonucleic acid (mRNA) levels of all subtypes using a semiquantitative RT-PCR and characterized pituitary membrane receptors using subtype-preferential SRIF analogs. Most of the SRIF receptor subtype mRNAs were sst5 and sst2A in ovariectomized rat pituitaries [sst5/glyceraldehyde 3-phosphate dehydrogenase (GAPDH) = 1.4 x 10-2, sst2A/GAPDH = 0.4 x 10-2]. The expression pattern of the subtypes in ovariectomized rat pituitaries was similar to that of normal male and female rat pituitaries, although the mRNA levels of sst5 and sst2A in male rat pituitaries were higher than in females. Chronic administration (4 weeks) of E2 to the ovariectomized rats increased mRNA expression of sst2A, sst2B, sst3, and sst1 and drastically decreased expression of sst5; the transcripts of sst2 isoforms constituted 87% of total SRIF receptor subtype mRNAs (sst2A/GAPDH = 1.2 x 10-2), whereas the sst5 mRNA level was less than 1%. Receptor-binding studies revealed that in pituitaries from both ovariectomized rats and male rats, heterogeneous receptor types, probably sst5 and sst2, were expressed, whereas receptors from E2-treated rat pituitaries mostly exhibited characteristics of the sst2 subtype. The results demonstrated that sst5 and sst2A were the major subtypes expressed in normal rat pituitaries with a sex-dependent difference and that whereas E2 up-regulates the expression of sst2 isoforms, it down-regulates the expression of sst5, suggesting roles for these subtypes in the control of pituitary functions.




This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
D. Zhang, J. T. Popesku, C. J. Martyniuk, H. Xiong, P. Duarte-Guterman, L. Yao, X. Xia, and V. L. Trudeau
Profiling neuroendocrine gene expression changes following fadrozole-induced estrogen decline in the female goldfish
Physiol Genomics, August 7, 2009; 38(3): 351 - 361.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. D. Veldhuis, K. L. Mielke, M. Cosma, C. Soares-Welch, R. Paulo, J. M. Miles, and C. Y. Bowers
Aromatase and 5{alpha}-Reductase Inhibition during an Exogenous Testosterone Clamp Unveils Selective Sex Steroid Modulation of Somatostatin and Growth Hormone Secretagogue Actions in Healthy Older Men
J. Clin. Endocrinol. Metab., March 1, 2009; 94(3): 973 - 981.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
J. D Veldhuis and D. M Keenan
Secretagogues govern GH secretory-burst waveform and mass in healthy eugonadal and short-term hypogonadal men
Eur. J. Endocrinol., November 1, 2008; 159(5): 547 - 554.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. D. Veldhuis, D. M. Keenan, J. N. Bailey, A. Adeniji, J. M. Miles, R. Paulo, M. Cosma, and C. Soares-Welch
Estradiol Supplementation in Postmenopausal Women Attenuates Suppression of Pulsatile Growth Hormone Secretion by Recombinant Human Insulin-like Growth Factor Type I
J. Clin. Endocrinol. Metab., November 1, 2008; 93(11): 4471 - 4478.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Cosma, J. Bailey, J. M. Miles, C. Y. Bowers, and J. D. Veldhuis
Pituitary and/or Peripheral Estrogen-Receptor {alpha} Regulates Follicle-Stimulating Hormone Secretion, Whereas Central Estrogenic Pathways Direct Growth Hormone and Prolactin Secretion in Postmenopausal Women
J. Clin. Endocrinol. Metab., March 1, 2008; 93(3): 951 - 958.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
N. Kimura, N. Takamatsu, Y. Yaoita, R Y. Osamura, and N. Kimura
Identification of transcriptional regulatory elements in the human somatostatin receptor sst2 promoter and regions including estrogen response element half-site for estrogen activation
J. Mol. Endocrinol., February 1, 2008; 40(2): 75 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. D. Veldhuis, D. M. Keenan, and C. Y. Bowers
Peripheral estrogen receptor-{alpha} selectively modulates the waveform of GH secretory bursts in healthy women
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1514 - R1521.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. D. Veldhuis, D. M. Keenan, A. Iranmanesh, K. Mielke, J. M. Miles, and C. Y. Bowers
Estradiol Potentiates Ghrelin-Stimulated Pulsatile Growth Hormone Secretion in Postmenopausal Women
J. Clin. Endocrinol. Metab., September 1, 2006; 91(9): 3559 - 3565.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. D. Veldhuis, J. N. Roemmich, E. J. Richmond, and C. Y. Bowers
Somatotropic and Gonadotropic Axes Linkages in Infancy, Childhood, and the Puberty-Adult Transition
Endocr. Rev., April 1, 2006; 27(2): 101 - 140.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
C. A. Blake, L. M. Brown, M. W. Duncan, S. W. Hunsucker, and S. M. Helmke
Estrogen Regulation of the Rat Anterior Pituitary Gland Proteome
Experimental Biology and Medicine, December 1, 2005; 230(11): 800 - 807.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. D. Veldhuis, S. M. Anderson, A. Iranmanesh, and C. Y. Bowers
Testosterone Blunts Feedback Inhibition of Growth Hormone Secretion by Experimentally Elevated Insulin-Like Growth Factor-I Concentrations
J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1613 - 1617.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
D. Erickson, D. M. Keenan, K. Mielke, K. Bradford, C. Y. Bowers, J. M. Miles, and J. D. Veldhuis
Dual Secretagogue Drive of Burst-Like Growth Hormone Secretion in Postmenopausal Compared with Premenopausal Women Studied under an Experimental Estradiol Clamp
J. Clin. Endocrinol. Metab., September 1, 2004; 89(9): 4746 - 4754.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Yan, M. E. E. Jones, M. Hernandez, D. Liu, E. R. Simpson, and C. Chen
Functional Modification of Pituitary Somatotropes in the Aromatase Knockout Mouse and the Effect of Estrogen Replacement
Endocrinology, February 1, 2004; 145(2): 604 - 612.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
L. J. Hofland and S. W. J. Lamberts
The Pathophysiological Consequences of Somatostatin Receptor Internalization and Resistance
Endocr. Rev., February 1, 2003; 24(1): 28 - 47.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
W. W. Woodmansee, R. L. Mouser, D. F. Gordon, J. M. Dowding, W. M. Wood, and E. C. Ridgway
Mutational Analysis of the Mouse Somatostatin Receptor Type 5 Gene Promoter
Endocrinology, June 1, 2002; 143(6): 2268 - 2276.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Diaz-Torga, C. Feierstein, C. Libertun, D. Gelman, M. A. Kelly, M. J. Low, M. Rubinstein, and D. Becu-Villalobos
Disruption of the D2 Dopamine Receptor Alters GH and IGF-I Secretion and Causes Dwarfism in Male Mice
Endocrinology, April 1, 2002; 143(4): 1270 - 1279.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Kimura, S. Tomizawa, K. N. Arai, R. Y. Osamura, and N. Kimura
Characterization of 5'-Flanking Region of Rat Somatostatin Receptor sst2 Gene: Transcriptional Regulatory Elements and Activation by Pitx1 and Estrogen
Endocrinology, April 1, 2001; 142(4): 1427 - 1441.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
S. Park, J. Kamegai, T. A. Johnson, L. A. Frohman, and R. D. Kineman
Modulation of Pituitary Somatostatin Receptor Subtype (sst1-5) Messenger Ribonucleic Acid Levels by Changes in the Growth Hormone Axis
Endocrinology, October 1, 2000; 141(10): 3556 - 3563.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society