| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6
Address all correspondence and requests for reprints to: Dr. Jean Sirois, Centre de Recherche en Reproduction Animale, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Québec, Canada J2S 7C6. E-mail: siroisje{at}medvet.umontreal.ca
To increase our understanding of the molecular control of PG synthesis
in equine preovulatory follicles, the specific objectives of this study
were to clone and determine the primary structure of equine
prostaglandin G/H synthase-2 (PGHS-2) and to characterize the
regulation of PGHS-2 messenger RNA (mRNA) in follicles before
ovulation. A complementary DNA (cDNA) library prepared from follicular
mRNA and a genomic library were screened with a mouse PGHS-2 cDNA probe
to isolate the equine PGHS-2 cDNA and gene, respectively. The
expression library yielded three nearly full-length clones that
differed only in their 5'-ends; clones 3, 5, and 6 were 2946, 3138, and
3398 bp in length, respectively. The longest clone was shown to start 9
bp downstream of the transcription initiation site, as determined by
primer extension analysis, and to contain 120 bp of 5'-untranslated
region (UTR), 1812 bp of open reading frame, and 1466 bp of 3'-UTR. The
open reading frame encodes a 604-amino acid protein that is more than
80% identical to PGHS-2 homologs in other species. Numerous repeats
(n = 11) of the Shaw-Kamen’s sequence (ATTTA) are present in the
3'-UTR, a motif typically indicative of mRNAs with a short half-life.
The complete equine PGHS-2 gene was isolated and sequenced from a
17-kilobase clone obtained from the genomic library. The equine
PGHS-2 gene structure (10 exons and 9 introns; total length of 6991 bp)
is similar to its human homolog except for lacking sequence elements in
introns 4, 8, and 9 and in the 3'-UTR region of exon 10. To
characterize the regulation of PGHS-2 mRNA in equine follicles before
ovulation, preovulatory follicles were isolated during estrus, 0, 12,
24, 30, 33, 36, and 39 h (n = 4–5 follicles/time point)
after an ovulatory dose of hCG. Results from Northern blots showed
significant changes in steady state levels of PGHS-2 mRNA in
preovulatory follicles after hCG treatment (P <
0.05). The transcript remained undetectable between 0–24 h post-hCG,
first appeared (4 kilobases) only at 30 h, and reached maximal
levels 33 h post-hCG. PGHS-2 mRNA was selectively induced in
granulosa cells and not in theca interna. Thus, this study provides for
the first time the primary structure of the equine PGHS-2 gene,
transcript, and protein. It also demonstrates that the induction of
PGHS-2 gene expression in equine granulosa cells is a long molecular
process (30 h post-hCG), thereby providing a model to study the
molecular basis for the late transcriptional activation of PGHS-2 in
species with a long ovulatory process.
This article has been cited by other articles:
 |
|
 |
|
  J. Liu, E.-S. Park, and M. Jo Runt-Related Transcription Factor 1 Regulates Luteinized Hormone-Induced Prostaglandin-Endoperoxide Synthase 2 Expression in Rat Periovulatory Granulosa Cells Endocrinology, July 1, 2009; 150(7): 3291 - 3300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sayasith, J. Sirois, and M. Dore Molecular Characterization of Feline COX-2 and Expression in Feline Mammary Carcinomas Vet. Pathol., May 1, 2009; 46(3): 423 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markosyan and D. M. Duffy Prostaglandin E2 Acts via Multiple Receptors to Regulate Plasminogen-Dependent Proteolysis in the Primate Periovulatory Follicle Endocrinology, January 1, 2009; 150(1): 435 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-N. Lague, M. Paquet, H.-Y. Fan, M. J. Kaartinen, S. Chu, S. P. Jamin, R. R. Behringer, P. J. Fuller, A. Mitchell, M. Dore, et al. Synergistic effects of Pten loss and WNT/CTNNB1 signaling pathway activation in ovarian granulosa cell tumor development and progression Carcinogenesis, November 1, 2008; 29(11): 2062 - 2072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Feuerstein, V. Cadoret, R. Dalbies-Tran, F. Guerif, R. Bidault, and D. Royere Gene expression in human cumulus cells: one approach to oocyte competence Hum. Reprod., December 1, 2007; 22(12): 3069 - 3077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sayasith, N. Bouchard, M. Dore, and J. Sirois Cloning of equine prostaglandin dehydrogenase and its gonadotropin-dependent regulation in theca and mural granulosa cells of equine preovulatory follicles during the ovulatory process Reproduction, February 1, 2007; 133(2): 455 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sayasith, M. Dore, and J. Sirois Molecular characterization of tumor necrosis {alpha}-induced protein 6 and its human chorionic gonadotropin-dependent induction in theca and mural granulosa cells of equine preovulatory follicles Reproduction, January 1, 2007; 133(1): 135 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Boerboom, M. Paquet, M. Hsieh, J. Liu, S. P. Jamin, R. R. Behringer, J. Sirois, M. M. Taketo, and J. S. Richards Misregulated Wnt/{beta}-Catenin Signaling Leads to Ovarian Granulosa Cell Tumor Development Cancer Res., October 15, 2005; 65(20): 9206 - 9215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sayasith, N. Bouchard, D. Boerboom, K. A. Brown, M. Dore, and J. Sirois Molecular Characterization of Equine P-Selectin (CD62P) and Its Regulation in Ovarian Follicles During the Ovulatory Process Biol Reprod, March 1, 2005; 72(3): 736 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sirois, K. Sayasith, K. A. Brown, A. E. Stock, N. Bouchard, and M. Dore Cyclooxygenase-2 and its role in ovulation: a 2004 account Hum. Reprod. Update, September 1, 2004; 10(5): 373 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Jarving, I. Jarving, R. Kurg, A. R. Brash, and N. Samel On the Evolutionary Origin of Cyclooxygenase (COX) Isozymes: CHARACTERIZATION OF MARINE INVERTEBRATE COX GENES POINTS TO INDEPENDENT DUPLICATION EVENTS IN VERTEBRATE AND INVERTEBRATE LINEAGES J. Biol. Chem., April 2, 2004; 279(14): 13624 - 13633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Brown, D. Boerboom, N. Bouchard, M. Dore, J. G. Lussier, and J. Sirois Human Chorionic Gonadotropin-Dependent Regulation of 17{beta}-Hydroxysteroid Dehydrogenase Type 4 in Preovulatory Follicles and Its Potential Role in Follicular Luteinization Endocrinology, April 1, 2004; 145(4): 1906 - 1915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sayasith, N. Bouchard, M. Sawadogo, J. G. Lussier, and J. Sirois Molecular Characterization and Role of Bovine Upstream Stimulatory Factor 1 and 2 in the Regulation of the Prostaglandin G/H Synthase-2 Promoter in Granulosa Cells J. Biol. Chem., February 20, 2004; 279(8): 6327 - 6336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Boerboom, K. A. Brown, D. Vaillancourt, P. Poitras, A. K. Goff, K. Watanabe, M. Dore, and J. Sirois Expression of Key Prostaglandin Synthases in Equine Endometrium During Late Diestrus and Early Pregnancy Biol Reprod, February 1, 2004; 70(2): 391 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Segi, K. Haraguchi, Y. Sugimoto, M. Tsuji, H. Tsunekawa, S. Tamba, K. Tsuboi, S. Tanaka, and A. Ichikawa Expression of Messenger RNA for Prostaglandin E Receptor Subtypes EP4/EP2 and Cyclooxygenase Isozymes in Mouse Periovulatory Follicles and Oviducts During Superovulation Biol Reprod, March 1, 2003; 68(3): 804 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Stock, N. Bouchard, K. Brown, A. P. Spicer, C. B. Underhill, M. Dore, and J. Sirois Induction of Hyaluronan Synthase 2 by Human Chorionic Gonadotropin in Mural Granulosa Cells of Equine Preovulatory Follicles Endocrinology, November 1, 2002; 143(11): 4375 - 4384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lajoie, J. Sirois, and M. Dore Induction of Cyclo-oxygenase-2 Expression in Naturally Occurring Gastric Ulcers J. Histochem. Cytochem., July 1, 2002; 50(7): 923 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chu, S. Rushdi, E.T. Zumpe, P. Mamers, D.L. Healy, T. Jobling, H.G. Burger, and P.J. Fuller FSH-regulated gene expression profiles in ovarian tumours and normal ovaries Mol. Hum. Reprod., May 1, 2002; 8(5): 426 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Joyce, F. L. Pendola, M. O'Brien, and J. J. Eppig Regulation of Prostaglandin-Endoperoxide Synthase 2 Messenger Ribonucleic Acid Expression in Mouse Granulosa Cells during Ovulation Endocrinology, July 1, 2001; 142(7): 3187 - 3197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, M. Antaya, A. K. Goff, D. Boerboom, D. W. Silversides, J. G. Lussier, and J. Sirois Molecular Characterization of Bovine Prostaglandin G/H Synthase-2 and Regulation in Uterine Stromal Cells Biol Reprod, March 1, 2001; 64(3): 983 - 991. [Abstract] [Full Text]
|
|
|
|
|
|
D. Boerboom and J. Sirois Equine P450 Cholesterol Side-Chain Cleavage and 3{beta}-Hydroxysteroid Dehydrogenase/{{Delta}}5-{{Delta}}4 Isomerase: Molecular Cloning and Regulation of Their Messenger Ribonucleic Acids in Equine Follicles During the Ovulatory Process Biol Reprod, January 1, 2001; 64(1): 206 - 215. [Abstract] [Full Text]
|
|
|
|
|
|
D. Boerboom, N. Pilon, R. Behdjani, D. W. Silversides, and J. Sirois Expression and Regulation of Transcripts Encoding Two Members of the NR5A Nuclear Receptor Subfamily of Orphan Nuclear Receptors, Steroidogenic Factor-1 and NR5A2, in Equine Ovarian Cells during the Ovulatory Process Endocrinology, December 1, 2000; 141(12): 4647 - 4656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, M. Antaya, D. Boerboom, J. G. Lussier, D. W. Silversides, and J. Sirois The Delayed Activation of the Prostaglandin G/H Synthase-2 Promoter in Bovine Granulosa Cells Is Associated with Down-regulation of Truncated Upstream Stimulatory Factor-2 J. Biol. Chem., December 3, 1999; 274(49): 35037 - 35045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Boerboom, A. Kerban, and J. Sirois Dual Regulation of Promoter II- and Promoter 1f-Derived Cytochrome P450 Aromatase Transcripts in Equine Granulosa Cells during Human Chorionic Gonadotropin-Induced Ovulation: A Novel Model for the Study of Aromatase Promoter Switching Endocrinology, September 1, 1999; 140(9): 4133 - 4141. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kerban, D. Boerboom, and J. Sirois Human Chorionic Gonadotropin Induces an Inverse Regulation of Steroidogenic Acute Regulatory Protein Messenger Ribonucleic Acid in Theca Interna and Granulosa Cells of Equine Preovulatory Follicles Endocrinology, February 1, 1999; 140(2): 667 - 674. [Abstract] [Full Text]
|
|
|
|
|
|
F. Filion, N. Bouchard, A. K. Goff, J. G. Lussier, and J. Sirois Molecular Cloning and Induction of Bovine Prostaglandin E Synthase by Gonadotropins in Ovarian Follicles Prior to Ovulation in Vivo J. Biol. Chem., August 31, 2001; 276(36): 34323 - 34330. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
