This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pati, D. Articles by Habibi, H. R. Search for Related Content PubMed PubMed Citation Articles by Pati, D. Articles by Habibi, H. R. Pubmed/NCBI databases Compound via MeSH Substance via MeSH

Presence of Salmon Gonadotropin-Releasing Hormone (GnRH) and Compounds with GnRH-Like Activity in the Ovary of Goldfish¹

Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4

Address all correspondence and requests for reprints to: Dr. H. R. Habibi, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4. E-mail: habibi{at}acs.ucalgary.ca

The presence of ovarian GnRH and/or compounds with GnRH-like activity was investigated in the goldfish ovary. Goldfish ovary was extracted using an acetone/HCl/ether mixture and was purified by Waters C-18 Sep-Pak columns (ovarian extract). The goldfish ovarian extract was found to 1) stimulate gonadotropin release and subunit messenger RNA production in the goldfish pituitary that was inhibited by a GnRH antagonist; 2) stimulate germinal vesicle breakdown in the prophase-I arrested follicle-enclosed goldfish oocytes in vitro, which was inhibited by a GnRH antagonist; 3) immunoreact with various GnRH antisera; and 4) bind to GnRH receptors in the goldfish pituitary and ovary as well as rat pituitary. Further purification with HPLC revealed the presence of two compounds with GnRH-like activity. One with identical chromatographic characteristics, amino acid composition, and primary structure to that of the salmon GnRH (sGnRH), and the other a novel compound with GnRH-like activity.

This article has been cited by other articles:

				A. Chambery, A. Parente, E. Topo, J. Garcia-Fernandez, and S. D'Aniello Characterization and Putative Role of a Type I Gonadotropin-Releasing Hormone in the Cephalochordate Amphioxus Endocrinology, February 1, 2009; 150(2): 812 - 820. [Abstract] [Full Text] [PDF]

				P. Singh, A. Krishna, and R. Sridaran Localization of gonadotrophin-releasing hormone I, bradykinin and their receptors in the ovaries of non-mammalian vertebrates Reproduction, May 1, 2007; 133(5): 969 - 981. [Abstract] [Full Text] [PDF]

				W.-H. Twan, J.-S. Hwang, Y.-H. Lee, S.-R. Jeng, W.-S. Yueh, Y.-H. Tung, H.-F. Wu, S. Dufour, and C.-F. Chang The Presence and Ancestral Role of Gonadotropin-Releasing Hormone in the Reproduction of Scleractinian Coral, Euphyllia ancora Endocrinology, January 1, 2006; 147(1): 397 - 406. [Abstract] [Full Text] [PDF]

				J. Bogerd, W. B. Diepenbroek, E. Hund, F. van Oosterhout, A. C. C. Teves, R. Leurs, and M. Blomenrohr Two Gonadotropin-Releasing Hormone Receptors in the African Catfish: No Differences in Ligand Selectivity, but Differences in Tissue Distribution Endocrinology, December 1, 2002; 143(12): 4673 - 4682. [Abstract] [Full Text] [PDF]

				S. L. Gray, B. A. Adams, C. M. Warby, K. R. von Schalburg, and N. M. Sherwood Transcription and Translation of the Salmon Gonadotropin-Releasing Hormone Genes in Brain and Gonads of Sexually Maturing Rainbow Trout (Oncorhynchus mykiss) Biol Reprod, November 1, 2002; 67(5): 1621 - 1627. [Abstract] [Full Text] [PDF]

				D. Pati and H. R. Habibi Involvement of Protein Kinase C and Arachidonic Acid Pathways in the Gonadotropin-Releasing Hormone Regulation of Oocyte Meiosis and Follicular Steroidogenesis in the Goldfish Ovary Biol Reprod, March 1, 2002; 66(3): 813 - 822. [Abstract] [Full Text] [PDF]

				M. Nabissi, L. Soverchia, A. M. Polzonetti-Magni, and H. R. Habibi Differential Splicing of Three Gonadotropin-Releasing Hormone Transcripts in the Ovary of Seabream (Sparus aurata) Biol Reprod, May 1, 2000; 62(5): 1329 - 1334. [Abstract] [Full Text]

				K. R. von Schalburg, C. M. Warby, and N. M. Sherwood Evidence for Gonadotropin-Releasing Hormone Peptides in the Ovary and Testis of Rainbow Trout Biol Reprod, June 1, 1999; 60(6): 1338 - 1344. [Abstract] [Full Text]

				N. Illing, B. E. Troskie, C. S. Nahorniak, J. P. Hapgood, R. E. Peter, and R. P. Millar Two gonadotropin-releasing hormone receptor subtypes with distinct ligand selectivity and differential distribution in brain and pituitary in the goldfish (Carassius auratus) PNAS, March 2, 1999; 96(5): 2526 - 2531. [Abstract] [Full Text] [PDF]

				M. M. Di Fiore, R. K. Rastogi, F. Ceciliani, E. Messi, V. Botte, L. Botte, C. Pinelli, B. D'Aniello, and A. D'Aniello Mammalian and chicken I forms of gonadotropin-releasing hormone in the gonads of a protochordate, Ciona intestinalis PNAS, February 29, 2000; 97(5): 2343 - 2348. [Abstract] [Full Text] [PDF]