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Effect of 17ß-Estradiol on Somatostatin Receptor Expression and Inhibitory Effects on Growth Hormone and Prolactin Release in Rat Pituitary Cell Cultures

INSERM U-159, Centre Paul Broca, 75014 Paris, France

Address all correspondence and requests for reprints to: Dr. J. Epelbaum, INSERM U-159, 2ter rue d’Alésia, 75014 Paris, France. E-mail: epelbaum{at}broca.inserm.fr

In the present study, we tested whether 17ß-estradiol (E₂)-induced PRL sensitivity to somatostatin-14 (SRIF) involves selective up-regulation of discrete somatostatin receptor subtypes (ssts) in primary cultures of female rat pituitary cells. The efficacy of the endogenous peptide SRIF to inhibit GH and PRL secretion and cAMP accumulation was compared with those of octreotide (OCT), BIM-23052, BIM-23056, and BIM-23268, which have been reported to be relatively selective for rat sst2, sst3, and sst5. Experiments were performed in steroid-depleted media supplemented or not with 1 nM E₂ for 96 h. SRIF, OCT, and BIM-23052 inhibited cAMP accumulation and GH release independently of E₂. In contrast, all three agonists affected PRL release in E₂-treated cultures only. Inhibition of cAMP accumulation by SRIF, OCT, and BIM-23052 was enhanced by exposure of cells to E₂. The rank of potency of the agonists, OCT = SRIF > BIM-23052, was similar for GH and PRL inhibition. BIM-23268 was a weak agonist on GH, but not on PRL, secretion. BIM-23056 had no effect on the release of either hormone, but slightly inhibited cAMP formation in E₂-treated cells. To verify whether SRIF receptor gene expression correlated with these observations, messenger RNA (mRNA) transcripts corresponding to the five ssts were measured by quantitative RT-PCR in the presence or absence of E₂. Control cells expressed predominantly sst2 and sst3 transcripts; sstl mRNA was present in moderate amounts, whereas sst4 and sst5 were only weakly expressed. E₂ had a differential effect on distinct ssts; it increased mRNA concentrations corresponding to sst2 and sst3, and decreased that of sst1. These results indicate that sst2 and sst3 receptors are the major somatostatin receptors expressed in the female rat pituitary, and that both of them are positively regulated by estradiol. However, the capacity of lactotropes to respond to SRIF after exposure to E₂ seems to depend more upon E₂-induced up-regulation of the sst2 than of the sst3 receptor subtype.

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