help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carlsson, L.
Right arrow Articles by Oscarsson, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carlsson, L.
Right arrow Articles by Oscarsson, J.
Endocrinology Vol. 139, No. 6 2699-2709
Copyright © 1998 by The Endocrine Society

ARTICLES

Hormonal Regulation of Liver Fatty Acid-Binding Protein in Vivo and in Vitro: Effects of Growth Hormone and Insulin1

Linda Carlsson, Ida Nilsson and Jan Oscarsson

Department of Physiology, Göteborg University, S-405 30 Göteborg, Sweden

Address all correspondence and requests for reprints to: Dr. Jan Oscarsson, Department of Physiology, Göteborg University, Box 434, Medicinaregatan 1F, S-405 30 Göteborg, Sweden. E-mail: jan.oscarsson{at}fysiologi.gu.se

Liver fatty acid-binding protein (LFABP) is an abundant protein in hepatocytes that binds most of the long chain fatty acids present in the cytosol. It is suggested to be of importance for fatty acid uptake and utilization in the hepatocyte. In the present study, the effects of bovine GH (bGH) and other hormones on the expression of LFABP and its messenger RNA (mRNA) were studied in hypophysectomized rats and in vitro using primary cultures of rat hepatocytes. One injection of bGH increased LFABP mRNA levels about 5-fold after 6 h, but there was no effect of this treatment on LFABP levels. However, 7 days of bGH treatment increased both LFABP mRNA and LFABP protein levels 2- to 5-fold. Female rats had higher levels of LFABP than male rats. Hypophysectomy of female rats, but not that of male rats, decreased LFABP levels markedly. Treatment of hypophysectomized rats with bGH for 7 days as two daily injections or as a continuous infusion increased LFABP levels to a similar degree. This finding indicates that the sex difference in the expression of LFABP is not regulated by the sexually dimorphic secretory pattern of GH. Neither insulin nor insulin-like growth factor I treatment of hypophysectomized rats for 6–7 days had any effect on LFABP mRNA or LFABP levels. In vitro, bGH dose-dependently increased the expression of LFABP mRNA, but only in the presence of insulin. Insulin alone had a marked dose-dependent effect on LFABP mRNA levels and was of importance for maintaining the expression of LFABP mRNA during the culture. Incubation with bGH increased LFABP mRNA levels within 3 h. GH had no effect on LFABP mRNA levels in the presence of actinomycin D, indicating a transcriptional effect of GH. Incubation with glucagon in vitro decreased LFABP mRNA levels markedly, indicating that glucagon, in contrast to GH, has an effect opposite that of insulin on LFABP mRNA expression. It is concluded that GH is an important regulator of LFABP in vivo and in vitro. In contrast to the effect of GH on insulin-like growth factor I mRNA, the presence of insulin was a prerequisite for the effect of GH on LFABP mRNA expression in vitro. The results emphasize the role of GH in the regulation of hepatic fatty acid metabolism.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Fernandez, M. Lindholm, M. Krogh, S. Lucas, S. Larsson, P. Osmark, K. Berger, J. Boren, B. Fielding, K. Frayn, et al.
Disturbed cholesterol homeostasis in hormone-sensitive lipase-null mice
Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E820 - E831.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Eleswarapu, Z. Gu, and H. Jiang
Growth Hormone Regulation of Insulin-Like Growth Factor-I Gene Expression May Be Mediated by Multiple Distal Signal Transducer and Activator of Transcription 5 Binding Sites
Endocrinology, May 1, 2008; 149(5): 2230 - 2240.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. D. Aberg, U. E. Johansson, M. A. I. Aberg, N. A. K. Hellstrom, J. Lind, C. Bull, J. Isgaard, M. F. Anderson, J. Oscarsson, and P. S. Eriksson
Peripheral Infusion of Insulin-Like Growth Factor-I Increases the Number of Newborn Oligodendrocytes in the Cerebral Cortex of Adult Hypophysectomized Rats
Endocrinology, August 1, 2007; 148(8): 3765 - 3772.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
U. Edvardsson, A. Ljungberg, D. Linden, L. William-Olsson, H. Peilot-Sjogren, A. Ahnmark, and J. Oscarsson
PPAR{alpha} activation increases triglyceride mass and adipose differentiation-related protein in hepatocytes
J. Lipid Res., February 1, 2006; 47(2): 329 - 340.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Ameen, U. Edvardsson, A. Ljungberg, L. Asp, P. Akerblad, A. Tuneld, S.-O. Olofsson, D. Linden, and J. Oscarsson
Activation of Peroxisome Proliferator-activated Receptor {alpha} Increases the Expression and Activity of Microsomal Triglyceride Transfer Protein in the Liver
J. Biol. Chem., January 14, 2005; 280(2): 1224 - 1229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Ameen, D. Linden, B.-M. Larsson, A. Mode, A. Holmang, and J. Oscarsson
Effects of gender and GH secretory pattern on sterol regulatory element-binding protein-1c and its target genes in rat liver
Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1039 - E1048.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Matasconi, B. Angelin, and M. Rudling
Pituitary control of lipoprotein and bile acid metabolism in male rats: growth hormone effects are not mediated by prolactin
Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E114 - E119.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Linden, K. Lindberg, J. Oscarsson, C. Claesson, L. Asp, L. Li, M. Gustafsson, J. Boren, and S.-O. Olofsson
Influence of Peroxisome Proliferator-activated Receptor alpha Agonists on the Intracellular Turnover and Secretion of Apolipoprotein (Apo) B-100 and ApoB-48
J. Biol. Chem., June 14, 2002; 277(25): 23044 - 23053.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Linden, M. Alsterholm, H. Wennbo, and J. Oscarsson
PPAR{alpha} deficiency increases secretion and serum levels of apolipoprotein B-containing lipoproteins
J. Lipid Res., November 1, 2001; 42(11): 1831 - 1840.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Carlsson, D. Linden, M. Jalouli, and J. Oscarsson
Effects of fatty acids and growth hormone on liver fatty acid binding protein and PPAR{alpha} in rat liver
Am J Physiol Endocrinol Metab, October 1, 2001; 281(4): E772 - E781.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. Linden, A. Sjoberg, L. Asp, L. Carlsson, and J. Oscarsson
Direct effects of growth hormone on production and secretion of apolipoprotein B from rat hepatocytes
Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1335 - E1346.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
F. Frick, J. Oscarsson, K. Vikman-Adolfsson, M. Ottosson, N. Yoshida, and S. Eden
Different effects of IGF-I on insulin-stimulated glucose uptake in adipose tissue and skeletal muscle
Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E729 - E737.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society