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Department of Obstetrics and Gynecology, Center for Reproductive Biology (D.T.B., B.K.C., L.M.H.), and the Department of Physiology (R.G.G.), University of Edinburgh, Edinburgh, Scotland EH3 9EW, United Kingdom; and the Roslin Institute (Edinburgh) (R.W.), Roslin, Midlothian, Scotland EH 259PS, United Kingdom
Address all correspondence and requests for reprints to: Prof. David T. Baird, Department of Obstetrics and Gynecology, Center for Reproductive Biology, University of Edinburgh, 37 Chalmers Street, Edinburgh, Scotland EH3 9EW, United Kingdom.
We have previously demonstrated that ovarian function and fertility can be preserved in sheep after castration by autotransplantation of cryopreserved strips of ovarian cortex. In the current experiments we have investigated the long term survival of such grafts by detailed measurements of ovarian function for a period of nearly 2 yr after autotransplantation. After ovariectomy and transplantation of frozen/thawed grafts, the concentrations of FSH and LH rose to castrate levels for about 14 weeks before falling gradually to reach near-normal levels at about 60 weeks. In the breeding season from October 1994 to March 1995, all ewes had 5–10 estrous cycles that were similar in length to those in the 4 control ewes. Luteal function as indicated by the progesterone concentration was identical before and 11 months after transplantation. In contrast, the basal concentrations of FSH and LH were persistently raised throughout the luteal phase, but showed a normal decline during the follicular phase. The concentration of inhibin A in ovarian venous plasma measured at the end of the experiment 22 months after transplantation was significantly lower than that in control ewes (mean ± SE, 409 ± 118 vs. 1914 ± 555 pg/ml; P < 0.004). Transplantation of frozen/thawed ovarian tissue to SCID mice demonstrated that about 28% of primordial follicles survived the procedure. All of the ovaries transplanted into sheep contained large antral follicles and/or cysts, but very few primordial oocytes when recovered at autopsy after 22 months.
These results demonstrate that despite a drastic reduction in the total number of primordial follicles, cyclical ovarian function is preserved in sheep after autotransplantation of frozen/thawed ovarian tissue and provide experimental confirmation that such a technique could provide a means of preserving fertility in women undergoing chemo- or radiotherapy for malignant disease.
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C.G. Almodin, V.C. Minguetti-Camara, H. Meister, J.O.H.R. Ferreira, R.L. Franco, A.A. Cavalcante, M.R.M. Radaelli, A.S. Bahls, A.F. Moron, and C.G.V. Murta Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy Hum. Reprod., June 1, 2004; 19(6): 1287 - 1293. [Abstract] [Full Text] [PDF]
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S.S. Kim Ovarian tissue banking for cancer patients: To do or not to do? Hum. Reprod., September 1, 2003; 18(9): 1759 - 1761. [Abstract] [Full Text] [PDF]
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D. A. Gook, D.H. Edgar, J. Borg, J. Archer, P.J. Lutjen, and J.C. McBain Oocyte maturation, follicle rupture and luteinization in human cryopreserved ovarian tissue following xenografting Hum. Reprod., September 1, 2003; 18(9): 1772 - 1781. [Abstract] [Full Text] [PDF]
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H. Yin, X. Wang, S.S. Kim, H. Chen, S. L. Tan, and R. G. Gosden Transplantation of intact rat gonads using vascular anastomosis: effects of cryopreservation, ischaemia and genotype Hum. Reprod., June 1, 2003; 18(6): 1165 - 1172. [Abstract] [Full Text] [PDF]
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J. Liu, J. Van der Elst, R. Van den Broecke, and M. Dhont Early massive follicle loss and apoptosis in heterotopically grafted newborn mouse ovaries Hum. Reprod., March 1, 2002; 17(3): 605 - 611. [Abstract] [Full Text] [PDF]
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R.A. Cushman, C.M. Wahl, and J.E. Fortune Bovine ovarian cortical pieces grafted to chick embryonic membranes: A model for studies on the activation of primordial follicles Hum. Reprod., January 1, 2002; 17(1): 48 - 54. [Abstract] [Full Text] [PDF]
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S. S. Kim, J. Radford, M. Harris, J. Varley, A. J. Rutherford, B. Lieberman, S. Shalet, and R. Gosden Ovarian tissue harvested from lymphoma patients to preserve fertility may be safe for autotransplantation Hum. Reprod., October 1, 2001; 16(10): 2056 - 2060. [Abstract] [Full Text] [PDF]
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J. Callejo, C. Salvador, A. Miralles, S. Vilaseca, J. M. Lailla, and J. Balasch Long-Term Ovarian Function Evaluation after Autografting by Implantation with Fresh and Frozen-Thawed Human Ovarian Tissue J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4489 - 4494. [Abstract] [Full Text] [PDF]
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 R Grundy, R G Gosden, M Hewitt, V Larcher, A Leiper, H A Spoudeas, D Walker, and W H B Wallace Personal practice: Fertility preservation for children treated for cancer (1): scientific advances and research dilemmas Arch. Dis. Child., April 1, 2001; 84(4): 355 - 359. [Full Text]
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C.J. Candy, M.J. Wood, and D.G. Whittingham Restoration of a normal reproductive lifespan after grafting of cryopreserved mouse ovaries Hum. Reprod., June 1, 2000; 15(6): 1300 - 1304. [Abstract] [Full Text] [PDF]
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 H A Spoudeas, W H B Wallace, and D Walker Is germ cell harvest and storage justified in minors treated for cancer? BMJ, January 29, 2000; 320(7230): 316 - 316. [Full Text]
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 D. C. Linch, R. G. Gosden, T. Tulandi, S.-L. Tan, and S. L. Hancock Hodgkin's Lymphoma: Choice of Therapy and Late Complications Hematology, January 1, 2000; 2000(1): 205 - 221. [Abstract] [Full Text] [PDF]
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Y. Aubard, P. Piver, Y. Cognie, V. Fermeaux, N. Poulin, and M.A. Driancourt Orthotopic and heterotopic autografts of frozen–thawed ovarian cortex in sheep Hum. Reprod., August 1, 1999; 14(8): 2149 - 2154. [Abstract] [Full Text] [PDF]
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