| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Department of Cell Biology, Medical School, University of Utrecht (A.M.M.v.P., D.G.d.R.), and the Netherlands Institute for Developmental Biology, Hubrecht Laboratory (B.v.d.B., P.T.v.d.S.), Utrecht, The Netherlands; and the Center for Biotechnology and Department of Medical Nutrition, Karolinska Institute (J.-Å.G., G.G.J.M.K.), Huddinge, Sweden
Address all correspondence and requests for reprints to: Dr. A. M. M. van Pelt, Department of Cell Biology, Medical School, Utrecht University, AZU H02.314, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. E-mail: A.Pelt{at}pobox.accu.uu.nl
The recently discovered estrogen receptor-ß (ERß) is expressed in rodent and human testes. To obtain insight in the physiological role of ERß we have investigated the cell type-specific expression pattern of ERß messenger RNA (mRNA) and protein in the testis of rats of various ages by in situ hybridization and immunohistochemistry. In fetal testes of rats 16 days postcoitum and testes of 4-day-old animals, fetal germ cells (gonocytes) reveal the ERß mRNA in their cytoplasm and the ERß protein in their nucleus. In testes of 11- and 15-day-old rats, ERß mRNA and protein were detected in Sertoli cells and type A spermatogonia. No signal was found in other types of germ cells. In the adult testes, expression of ERß mRNA as well as ERß protein was found in pachytene spermatocytes from epithelial stages VII–XIV and in round spermatids from stages I–VIII. Low ERß expression was observed in all type A spermatogonia, including undifferentiated A spermatogonia, whereas no expression was found in In and type B spermatogonia and early spermatocytes. At all ages, Sertoli cells showed a weak hybridization signal as well as weak immunoreactivity for ERß. In adult testes, no ERß mRNA or protein was detected in the interstitial tissue, indicating that Leydig cells and peritubular myoid cells do not express ERß. The expression of ERß in fetal and late male germ cells as well as in Sertoli cells suggests that estrogens directly affect germ cells during testicular development and spermatogenesis.
This article has been cited by other articles:
 |
|
 |
|
  K. L. Porter, G. Shetty, G. A. Shuttlesworth, C. C. Y. Weng, I. Huhtaniemi, P. Pakarinen, and M. L. Meistrich Estrogen Enhances Recovery From Radiation-Induced Spermatogonial Arrest in Rat Testes J Androl, July 1, 2009; 30(4): 440 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Leung, M. Kawai, J. K. Tai, J. Chen, S. M. Bandiera, and T. K. H. Chang Developmental Expression and Endocrine Regulation of CYP1B1 in Rat Testis Drug Metab. Dispos., March 1, 2009; 37(3): 523 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Wahlgren, K. Svechnikov, M.-L. Strand, K. Jahnukainen, M. Parvinen, J.-A. Gustafsson, and O. Soder Estrogen Receptor {beta} Selective Ligand 5{alpha}-Androstane-3{beta}, 17{beta}-Diol Stimulates Spermatogonial Deoxyribonucleic Acid Synthesis in Rat Seminiferous Epithelium in Vitro Endocrinology, June 1, 2008; 149(6): 2917 - 2922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Warita, K. Okamoto, K.-i. Mutoh, Y. Hasegawa, Z.-P. Yue, T. Yokoyama, Y. Matsumoto, T. Miki, Y. Takeuchi, H. Kitagawa, et al. Activin A and Equine Chorionic Gonadotropin Recover Reproductive Dysfunction Induced by Neonatal Exposure to an Estrogenic Endocrine Disruptor in Adult Male Mice Biol Reprod, January 1, 2008; 78(1): 59 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F.G Lucas, E. R Siu, C. A Esteves, H. P Monteiro, C. A Oliveira, C. S Porto, and M. F. M Lazari 17Beta-Estradiol Induces the Translocation of the Estrogen Receptors ESR1 and ESR2 to the Cell Membrane, MAPK3/1 Phosphorylation and Proliferation of Cultured Immature Rat Sertoli Cells Biol Reprod, January 1, 2008; 78(1): 101 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Assinder, R. Davis, M. Fenwick, and A. Glover Adult-only exposure of male rats to a diet of high phytoestrogen content increases apoptosis of meiotic and post-meiotic germ cells Reproduction, January 1, 2007; 133(1): 11 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Delbes, C. Levacher, and R. Habert Estrogen effects on fetal and neonatal testicular development. Reproduction, October 1, 2006; 132(4): 527 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Stabile, M. Russo, and P. Chieffi 17{beta}-Estradiol induces Akt-1 through estrogen receptor-{beta} in the frog (Rana esculenta) male germ cells. Reproduction, September 1, 2006; 132(3): 477 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. M. Mikkila, J. Toppari, and J. Paranko Effects of Neonatal Exposure to 4-Tert-Octylphenol, Diethylstilbestrol, and Flutamide on Steroidogenesis in Infantile Rat Testis Toxicol. Sci., June 1, 2006; 91(2): 456 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Baines, M. O Nwagwu, E. C Furneaux, J. Stewart, J. B Kerr, T. M Mayhew, and F. J P Ebling Estrogenic induction of spermatogenesis in the hypogonadal (hpg) mouse: role of androgens Reproduction, November 1, 2005; 130(5): 643 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Naciff, K. A. Hess, G. J. Overmann, S. M. Torontali, G. J. Carr, J. P. Tiesman, L. M. Foertsch, B. D. Richardson, J. E. Martinez, and G. P. Daston Gene Expression Changes Induced in the Testis by Transplacental Exposure to High and Low Doses of 17{alpha}-Ethynyl Estradiol, Genistein, or Bisphenol A Toxicol. Sci., August 1, 2005; 86(2): 396 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Selva, O. M. Tirado, N. Toran, C. A. Suarez-Quian, J. Reventos, and F. Munell Estrogen Receptor {beta} Expression and Apoptosis of Spermatocytes of Mice Overexpressing a Rat Androgen-Binding Protein Transgene Biol Reprod, November 1, 2004; 71(5): 1461 - 1468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, R. Thuillier, and M. Culty Prenatal Estrogen Exposure Differentially Affects Estrogen Receptor-Associated Proteins in Rat Testis Gonocytes Biol Reprod, November 1, 2004; 71(5): 1652 - 1664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Shi, A. Z. Wu, M. Santos, Z.-M. Feng, L. Huang, Y.-M. Chen, K. Zhu, and C.-L. C. Chen Cloning and Characterization of Rat Spermatid Protein SSP411: A Thioredoxin-Like Protein J Androl, July 1, 2004; 25(4): 479 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Delbes, C. Levacher, C. Pairault, C. Racine, C. Duquenne, A. Krust, and R. Habert Estrogen Receptor {beta}-Mediated Inhibition of Male Germ Cell Line Development in Mice by Endogenous Estrogens during Perinatal Life Endocrinology, July 1, 2004; 145(7): 3395 - 3403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Albrecht, R. B. Billiar, G. W. Aberdeen, J. S. Babischkin, and G. J. Pepe Expression of Estrogen Receptors {alpha} and {beta} in the Fetal Baboon Testisand Epididymis Biol Reprod, April 1, 2004; 70(4): 1106 - 1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Akingbemi, C. M. Sottas, A. I. Koulova, G. R. Klinefelter, and M. P. Hardy Inhibition of Testicular Steroidogenesis by the Xenoestrogen Bisphenol A Is Associated with Reduced Pituitary Luteinizing Hormone Secretion and Decreased Steroidogenic Enzyme Gene Expression in Rat Leydig Cells Endocrinology, February 1, 2004; 145(2): 592 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Tirado, D. M. Selva, N. Toran, C. A. Suarez-Quian, M. Jansen, D. P. McDonnell, J. Reventos, and F. Munell Increased Expression of Estrogen Receptor {beta} in Pachytene Spermatocytes After Short-Term Methoxyacetic Acid Administration J Androl, January 1, 2004; 25(1): 84 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bourguiba, S. Chater, C. Delalande, M. Benahmed, and S. Carreau Regulation of Aromatase Gene Expression in Purified Germ Cells of Adult Male Rats: Effects of Transforming Growth Factor {beta}, Tumor Necrosis Factor {alpha}, and Cyclic Adenosine 3',5'-Monosphosphate Biol Reprod, August 1, 2003; 69(2): 592 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Fritz, M. S. Cotroneo, J. Wang, I.-E. Eltoum, and C. A. Lamartiniere Dietary Diethylstilbestrol but Not Genistein Adversely Affects Rat Testicular Development J. Nutr., July 1, 2003; 133(7): 2287 - 2293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.O. Goyal, A. Robateau, T.D. Braden, C.S. Williams, K.K. Srivastava, and K. Ali Neonatal Estrogen Exposure of Male Rats Alters Reproductive Functions at Adulthood Biol Reprod, June 1, 2003; 68(6): 2081 - 2091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lassurguere, G. Livera, R. Habert, and B. Jegou Time- and Dose-Related Effects of Estradiol and Diethylstilbestrol on the Morphology and Function of the Fetal Rat Testis in Culture Toxicol. Sci., May 1, 2003; 73(1): 160 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. van den Ham, F.M.F. van Dissel-Emiliani, and A.M.M. van Pelt Expression of the Scaffolding Subunit A of Protein Phosphatase 2A During Rat Testicular Development Biol Reprod, April 1, 2003; 68(4): 1369 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Thuillier, Y. Wang, and M. Culty Prenatal Exposure to Estrogenic Compounds Alters the Expression Pattern of Platelet-Derived Growth Factor Receptors {alpha} and {beta} in Neonatal Rat Testis: Identification of Gonocytes as Targets of Estrogen Exposure Biol Reprod, March 1, 2003; 68(3): 867 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Gaskell, L. L. L. Robinson, N. P. Groome, R. A. Anderson, and P. T. K. Saunders Differential Expression of Two Estrogen Receptor {beta} Isoforms in the Human Fetal Testis during the Second Trimester of Pregnancy J. Clin. Endocrinol. Metab., January 1, 2003; 88(1): 424 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rivas, J. S. Fisher, C. McKinnell, N. Atanassova, and R. M. Sharpe Induction of Reproductive Tract Developmental Abnormalities in the Male Rat by Lowering Androgen Production or Action in Combination with a Low Dose of Diethylstilbestrol: Evidence for Importance of the Androgen-Estrogen Balance Endocrinology, December 1, 2002; 143(12): 4797 - 4808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhou, R. Nie, G. S. Prins, P. T. K. Saunders, B. S. Katzenellenbogen, and R. A. Hess Localization of Androgen and Estrogen Receptors in Adult Male Mouse Reproductive Tract J Androl, November 1, 2002; 23(6): 870 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Fielden, R. G. Halgren, C. J. Fong, C. Staub, L. Johnson, K. Chou, and T. R. Zacharewski Gestational and Lactational Exposure of Male Mice to Diethylstilbestrol Causes Long-Term Effects on the Testis, Sperm Fertilizing Ability in Vitro, and Testicular Gene Expression Endocrinology, August 1, 2002; 143(8): 3044 - 3059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. van den Ham, F. M.F. van Dissel-Emiliani, and A. M.M. van Pelt Identification of Candidate Genes Involved in Gonocyte Development J Androl, May 1, 2002; 23(3): 410 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nie, Q. Zhou, E. Jassim, P. T.K. Saunders, and R. A. Hess Differential Expression of Estrogen Receptors {alpha} and {beta} in the Reproductive Tractsof Adult Male Dogs and Cats Biol Reprod, April 1, 2002; 66(4): 1161 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nilsson, S. Makela, E. Treuter, M. Tujague, J. Thomsen, G. Andersson, E. Enmark, K. Pettersson, M. Warner, and J.-A. Gustafsson Mechanisms of Estrogen Action Physiol Rev, October 1, 2001; 81(4): 1535 - 1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Oliveira, K. Carnes, L. R. Franca, and R. A. Hess Infertility and Testicular Atrophy in the Antiestrogen-Treated Adult Male Rat Biol Reprod, September 1, 2001; 65(3): 913 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bouma and J. J. Nagler Estrogen Receptor-{{alpha}} Protein Localization in the Testis of the Rainbow Trout (Oncorhynchus mykiss) During Different Stages of the Reproductive Cycle Biol Reprod, July 1, 2001; 65(1): 60 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O'Donnell, K. M. Robertson, M. E. Jones, and E. R. Simpson Estrogen and Spermatogenesis Endocr. Rev., June 1, 2001; 22(3): 289 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Makinen, S. Makela, Z. Weihua, M. Warner, B. Rosenlund, S. Salmi, O. Hovatta, and J.-A. Gustafsson Localization of oestrogen receptors alpha and beta in human testis Mol. Hum. Reprod., June 1, 2001; 7(6): 497 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Mansour, M. R. Machen, B. J. Tarleton, A. A. Wiley, J. Wower, F. F. Bartol, and H. O. Goyal Expression and Molecular Characterization of Estrogen Receptor Alpha Messenger RNA in Male Reproductive Organs of Adult Goats Biol Reprod, May 1, 2001; 64(5): 1432 - 1438. [Abstract] [Full Text]
|
|
|
|
|
|
P. T.K. Saunders, R. M. Sharpe, K. Williams, S. Macpherson, H. Urquart, D.S. Irvine, and M. R. Millar Differential expression of oestrogen receptor {{alpha}} and {beta} proteins in the testes and male reproductive system of human and non-human primates Mol. Hum. Reprod., March 1, 2001; 7(3): 227 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.O. Goyal, T.D. Braden, M. Mansour, C.S. Williams, A. Kamaleldin, and K.K. Srivastava Diethylstilbestrol-Treated Adult Rats with Altered Epididymal Sperm Numbers and Sperm Motility Parameters, but Without Alterations in Sperm Production and Sperm Morphology Biol Reprod, March 1, 2001; 64(3): 927 - 934. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Bendsen, S. B. Laursen, C. Olesen, L. G. Westergaard, C. Y. Andersen, and A. G. Byskov Effect of 4-octylphenol on germ cell number in cultured human fetal gonads Hum. Reprod., February 1, 2001; 16(2): 236 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Atanassova, C. McKinnell, K. J. Turner, M. Walker, J. S. Fisher, M. Morley, M. R. Millar, N. P. Groome, and R. M. Sharpe Comparative Effects of Neonatal Exposure of Male Rats to Potent and Weak (Environmental) Estrogens on Spermatogenesis at Puberty and the Relationship to Adult Testis Size and Fertility: Evidence for Stimulatory Effects of Low Estrogen Levels Endocrinology, October 1, 2000; 141(10): 3898 - 3907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pentikäinen, K. Erkkilä, L. Suomalainen, M. Parvinen, and L. Dunkel Estradiol Acts as a Germ Cell Survival Factor in the Human Testis in Vitro J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 2057 - 2067. [Abstract] [Full Text]
|
|
|
|
|
|
W. N. Jefferson, J. F. Couse, E. P. Banks, K. S. Korach, and R. R. Newbold Expression of Estrogen Receptor {beta} Is Developmentally Regulated in Reproductive Tissues of Male and Female Mice Biol Reprod, February 1, 2000; 62(2): 310 - 317. [Abstract] [Full Text]
|
|
|
|
|
|
N. Atanassova, C. McKinnell, M. Walker, K. J. Turner, J. S. Fisher, M. Morley, M. R. Millar, N. P. Groome, and R. M. Sharpe Permanent Effects of Neonatal Estrogen Exposure in Rats on Reproductive Hormone Levels, Sertoli Cell Number, and the Efficiency of Spermatogenesis in Adulthood Endocrinology, November 1, 1999; 140(11): 5364 - 5373. [Abstract] [Full Text]
|
|
|
|
|
|
P. Parma, E. Pailhoux, and C. Cotinot Reverse Transcription-Polymerase Chain Reaction Analysis of Genes Involved in Gonadal Differentiation in Pigs Biol Reprod, September 1, 1999; 61(3): 741 - 748. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. M. Robertson, L. O'Donnell, M. E. E. Jones, S. J. Meachem, W. C. Boon, C. R. Fisher, K. H. Graves, R. I. McLachlan, and E. R. Simpson Impairment of spermatogenesis in mice lacking a functional aromatase (cyp 19) gene PNAS, July 6, 1999; 96(14): 7986 - 7991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Couse and K. S. Korach Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us? Endocr. Rev., June 1, 1999; 20(3): 358 - 417. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Zhang, J. S. Thomsen, L. Johansson, J.-A. Gustafsson, and E. Treuter DAX-1 Functions as an LXXLL-containing Corepressor for Activated Estrogen Receptors J. Biol. Chem., December 15, 2000; 275(51): 39855 - 39859. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
