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Departments of Obstetrics & Gynecology (J.T., Sa.K.D.) and Molecular & Integrative Physiology (B.C.P., Su.K.D., Sa.K.D.), Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City, Kansas 66160-7338
Address all correspondence and requests for reprints to: S. K. Das, Departments of Obstetrics & Gynecology and Molecular & Integrative Physiology, MRRC 37/3004, University of Kansas Medical Center, 39th and Rainbow Boulevard, Kansas City, Kansas 66160-7338. E-mail: sdas{at}kumc.edu
The present investigation examined the spatiotemporal expression of
estrogen receptors (ER-
and ER-ß) and progesterone receptor (PR)
in the periimplantation mouse uterus (days 1–8). ER- messenger RNA
(mRNA) was detected at much higher levels in the periimplantation
uterus compared with that of ER-ß mRNA, the levels of which were very
low in all uterine cells during this period. Results of in
situ hybridization demonstrated expression of ER- mRNA
primarily in the luminal and glandular epithelia on days 1 and 2 of
pregnancy. On days 3 and 4, the accumulation was localized primarily in
stromal cells in addition to its presence in the epithelium. Following
implantation on day 5, the accumulation of this mRNA was more condensed
in the luminal and glandular epithelia, but declined in the subluminal
epithelial stroma at the sites of implanting embryos. On days 6–8, the
accumulation of ER- mRNA was primarily localized in the secondary
decidual zone (SDZ) with more intense localization in the subepithelial
cells at the mesometrial pole. In contrast, signals were very low to
undetectable in the primary decidual zone (PDZ), and no signals were
detected in implanting embryos. The undifferentiated stroma underneath
the myometrium also showed positive signals. The immunolocalization of
ER- protein correlated with the mRNA localization. Western blot
analysis showed down-regulation of ER- in day 8 decidual cell
extracts consistent with the down-regulation of ER- mRNA in decidual
cells immediately surrounding the embryo on this day. The expression
pattern of PR was also dynamic in the periimplantation uterus. On day
1, the accumulation of PR mRNA was very low to undetectable, whereas
only a modest level of accumulation in the epithelium was noted on day
2. On days 3 and 4, the accumulation of this mRNA was detected in both
the epithelium and stroma. In contrast, the expression was restricted
only to the stroma with increased signals at the sites of implantation
on day 5. On days 6–8, PR mRNA accumulation increased dramatically
throughout the deciduum. The localization of immunoreactive PR
correlated with the mRNA distribution in the periimplantation uterus.
Taken together, the results demonstrate that the expression of ER-,
ER-ß, and PR is differentially regulated in the periimplantation
mouse uterus. This compartmentalized expression of ER and PR provides
information regarding the sites of coordinated effects of estrogen and
progesterone in the preparation of the uterus for implantation and
decidualization during early pregnancy.
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C. Tessier, S. Deb, A. Prigent-Tessier, S. Ferguson-Gottschall, G. B. Gibori, R. P. C. Shiu, and G. Gibori Estrogen Receptors {alpha} and {beta} in Rat Decidua Cells: Cell-Specific Expression and Differential Regulation by Steroid Hormones and Prolactin Endocrinology, October 1, 2000; 141(10): 3842 - 3851. [Abstract] [Full Text] [PDF]
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H. Song, H. Lim, S. K. Das, B. C. Paria, and S. K. Dey Dysregulation of EGF Family of Growth Factors and COX-2 in the Uterus during the Preattachment and Attachment Reactions of the Blastocyst with the Luminal Epithelium Correlates with Implantation Failure in LIF- Deficient Mice Mol. Endocrinol., August 1, 2000; 14(8): 1147 - 1161. [Abstract] [Full Text]
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 C. Brisken, A. Heineman, T. Chavarria, B. Elenbaas, J. Tan, S. K. Dey, J. A. McMahon, A. P. McMahon, and R. A. Weinberg Essential function of Wnt-4 in mammary gland development downstream of progesterone signaling Genes & Dev., March 15, 2000; 14(6): 650 - 654. [Abstract] [Full Text]
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J.-G. Cheng, J. R. Chen, L. Hernandez, W. G. Alvord, and C. L. Stewart Dual control of LIF expression and LIF receptor function regulate Stat3 activation at the onset of uterine receptivity and embryo implantation PNAS, July 17, 2001; 98(15): 8680 - 8685. [Abstract] [Full Text] [PDF]
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