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Partial Characterization of Circulating Inhibin-B and Pro-C During Development in the Male Rhesus Monkey¹

Departments of Medicine and Physiology and Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213

Address all correspondence and requests for reprints to: Stephen J. Winters, M.D., Department of Medicine, University of Pittsburgh Medical Center, Montefiore N-919, 200 Lothrop Street, Pittsburgh Pennsylvania 15213. E-mail: winters{at}msx.dept-med.pitt.edu

Gel filtration chromatography and ELISAs for inhibin-B and pro-C were used to examine the circulating forms of inhibin in the neonatal (age 2–6 weeks), juvenile (age 1–2 yr), and adult male rhesus monkey. In all samples, isoforms of inhibin-B of 26–36K and 150K were found. Both forms were significantly greater in the adult. The -subunit assay detected major peaks at 45–60 and 29–31K, and a minor peak of greater than 100K. As for inhibin-B, the major forms of inhibin pro-C were highest in adulthood. Inhibin-B and pro-C were measurable in peripheral plasma at age 1 week, increased with the neonatal rise in plasma FSH, and then decreased but remained detectable through age 1 yr. Values in adult males were higher than at any time during the first year of life. Finally, mean values of plasma inhibin-B and pro-C in five monkeys, based on multiple blood samples drawn between age 1 week and 1 yr, were rank ordered and were found to be highly positively correlated (r = 0.96), suggesting that inhibin levels in the first year of life may be a marker of Sertoli cell number, and may predict the spermatogenic capacity of the testis in adulthood.

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