This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Winters, S. J. Articles by Plant, T. M. Search for Related Content PubMed PubMed Citation Articles by Winters, S. J. Articles by Plant, T. M.

Partial Characterization of Circulating Inhibin-B and Pro-C During Development in the Male Rhesus Monkey¹

Departments of Medicine and Physiology and Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213

Address all correspondence and requests for reprints to: Stephen J. Winters, M.D., Department of Medicine, University of Pittsburgh Medical Center, Montefiore N-919, 200 Lothrop Street, Pittsburgh Pennsylvania 15213. E-mail: winters{at}msx.dept-med.pitt.edu

Gel filtration chromatography and ELISAs for inhibin-B and pro-C were used to examine the circulating forms of inhibin in the neonatal (age 2–6 weeks), juvenile (age 1–2 yr), and adult male rhesus monkey. In all samples, isoforms of inhibin-B of 26–36K and 150K were found. Both forms were significantly greater in the adult. The -subunit assay detected major peaks at 45–60 and 29–31K, and a minor peak of greater than 100K. As for inhibin-B, the major forms of inhibin pro-C were highest in adulthood. Inhibin-B and pro-C were measurable in peripheral plasma at age 1 week, increased with the neonatal rise in plasma FSH, and then decreased but remained detectable through age 1 yr. Values in adult males were higher than at any time during the first year of life. Finally, mean values of plasma inhibin-B and pro-C in five monkeys, based on multiple blood samples drawn between age 1 week and 1 yr, were rank ordered and were found to be highly positively correlated (r = 0.96), suggesting that inhibin levels in the first year of life may be a marker of Sertoli cell number, and may predict the spermatogenic capacity of the testis in adulthood.

This article has been cited by other articles:

				L. Soriano-Guillen, V. Mitchell, J.-C. Carel, P. Barbet, M. Roger, and N. Lahlou Activating Mutations in the Luteinizing Hormone Receptor Gene: A Human Model of Non-Follicle-Stimulating Hormone-Dependent Inhibin Production and Germ Cell Maturation J. Clin. Endocrinol. Metab., August 1, 2006; 91(8): 3041 - 3047. [Abstract] [Full Text] [PDF]

				S. J. Winters, C. Wang, E. Abdelrahaman, V. Hadeed, M. A. Dyky, and A. Brufsky Inhibin-B Levels in Healthy Young Adult Men and Prepubertal Boys: Is Obesity the Cause for the Contemporary Decline in Sperm Count Because of Fewer Sertoli Cells? J Androl, July 1, 2006; 27(4): 560 - 564. [Abstract] [Full Text] [PDF]

				D. R. Simorangkir, G. R. Marshall, J. Ehmcke, S. Schlatt, and T. M. Plant Prepubertal Expansion of Dark and Pale Type A Spermatogonia in the Rhesus Monkey (Macaca mulatta) Results from Proliferation During Infantile and Juvenile Development in a Relatively Gonadotropin Independent Manner Biol Reprod, December 1, 2005; 73(6): 1109 - 1115. [Abstract] [Full Text] [PDF]

				S. J Winters and J. P Moore Intra-pituitary regulation of gonadotrophs in male rodents and primates Reproduction, July 1, 2004; 128(1): 13 - 23. [Abstract] [Full Text] [PDF]

				D. R. Simorangkir, G. R. Marshall, and T. M. Plant Sertoli Cell Proliferation during Prepubertal Development in the Rhesus Monkey (Macaca mulatta) Is Maximal during Infancy when Gonadotropin Secretion Is Robust J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4984 - 4989. [Abstract] [Full Text] [PDF]

				H. Kaneko, J. Noguchi, K. Kikuchi, and Y. Hasegawa Molecular Weight Forms of Inhibin A and Inhibin B in the Bovine Testis Change with Age Biol Reprod, May 1, 2003; 68(5): 1918 - 1925. [Abstract] [Full Text] [PDF]

				C.J.H. Kelnar, C. McKinnell, M. Walker, K.D. Morris, W.H.B. Wallace, P.T.K. Saunders, H.M. Fraser, and R.M. Sharpe Testicular changes during infantile 'quiescence' in the marmoset and their gonadotrophin dependence: a model for investigating susceptibility of the prepubertal human testis to cancer therapy? Hum. Reprod., May 1, 2002; 17(5): 1367 - 1378. [Abstract] [Full Text] [PDF]

				S. J. Winters, S. Kawakami, A. Sahu, and T. M. Plant Pituitary Follistatin and Activin Gene Expression, and the Testicular Regulation of FSH in the Adult Rhesus Monkey (Macaca mulatta) Endocrinology, July 1, 2001; 142(7): 2874 - 2878. [Abstract] [Full Text] [PDF]

				A.J. Tilbrook and I.J. Clarke Negative Feedback Regulation of the Secretion and Actions of Gonadotropin-Releasing Hormone in Males Biol Reprod, March 1, 2001; 64(3): 735 - 742. [Abstract] [Full Text]

				A.J. Tilbrook, D.M. de Kretser, and I.J. Clarke Influence of the Degree of Stimulation of the Pituitary by Gonadotropin-Releasing Hormone on the Action of Inhibin and Testosterone to Suppress the Secretion of the Gonadotropins in Rams Biol Reprod, February 1, 2001; 64(2): 473 - 481. [Abstract] [Full Text]