help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roland, B. L.
Right arrow Articles by Lovenberg, T. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roland, B. L.
Right arrow Articles by Lovenberg, T. W.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Endocrinology Vol. 140, No. 12 5736-5745
Copyright © 1999 by The Endocrine Society

ARTICLES

Anatomical Distribution of Prolactin-Releasing Peptide and Its Receptor Suggests Additional Functions in the Central Nervous System and Periphery

Barbara L. Roland, Steven W. Sutton, Sandy J. Wilson, Lin Luo, Jayashree Pyati, Rene Huvar, Mark G. Erlander and Timothy W. Lovenberg

R. W. Johnson Pharmaceutical Research Institute, San Diego, California 92121

Address all correspondence and requests for reprints to: Dr. Barbara L. Roland, R. W. Johnson Pharmaceutical Research Institute, 3210 Merryfield Row, San Diego, California 92121. E-mail: broland{at}prius jnj.com.

A recently identified neuropeptide with PRL-releasing capabilities binds to and activates a previously known orphan G protein-coupled receptor, GPR10. We initiated a study to define the pharmacology of the peptide/receptor interaction and to identify the distribution of the peptide and its receptor in the central nervous system to elucidate sites of action of the peptide. The PRL-releasing peptide (PrRP) is a C-terminally amidated, 31-amino acid peptide derived from a 98-amino acid precursor. Radioiodinated PrRP-(1–31) binds to its receptor with high affinity (1 nM) and stimulates calcium mobilization in CHOK1 cells stably transfected with the receptor. A series of N-terminal deletions reveals that the PrRP-(12–31) amino acid is equipotent to PrRP-(1–31). Further N-terminal deletions reduce the affinity of the ligand considerably, although PrRP-(25–31) is still able to compete for binding and behaves as an agonist. The arginine residues at position 26 and 30 are critical for binding, as substitution with either lysine or citrulline reduces the affinity substantially. In situ hybridization reveals a distinct tissue distribution for both the peptide and receptor messenger RNAs. The receptor is expressed abundantly in the reticular thalamic nucleus, periventricular hypothalamus, dorsomedial hypothalamus, nucleus of the solitary tract, area postrema, anterior pituitary, and adrenal medulla. The peptide messenger RNA is expressed in the dorsomedial hypothalamus, nucleus of the solitary tract, ventrolateral reticular nucleus, and intestine. This tissue distribution suggests an alternative function of PrRP than its purported hypophysiotropic function, such as a potential role for PrRP in the central feedback control of neuroendocrine and autonomic homeostasis. Further work using selective agonists and antagonists should help define additional physiological roles of this novel mammalian neuropeptide.




This article has been cited by other articles:


Home page
 J EndocrinolHome page
D. A Bechtold and S. M Luckman
The role of RFamide peptides in feeding
J. Endocrinol., January 1, 2007; 192(1): 3 - 15.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. Ohiwa, H. Chang, T. Saito, T. Onaka, T. Fujikawa, and H. Soya
Possible inhibitory role of prolactin-releasing peptide for ACTH release associated with running stress
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R497 - R504.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. P. Kelly and R. E. Peter
Prolactin-releasing peptide, food intake, and hydromineral balance in goldfish
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1474 - R1481.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. A. Bechtold and S. M. Luckman
Prolactin-Releasing Peptide Mediates Cholecystokinin-Induced Satiety in Mice
Endocrinology, October 1, 2006; 147(10): 4723 - 4729.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
T Nanmoku, K Takekoshi, T Fukuda, K Ishii, K Isobe, and Y Kawakami
Stimulation of catecholamine biosynthesis via the PKC pathway by prolactin-releasing peptide in PC12 rat pheochromocytoma cells
J. Endocrinol., July 1, 2005; 186(1): 233 - 239.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
T Sakamoto, M Amano, S Hyodo, S Moriyama, A Takahashi, H Kawauchi, and M Ando
Expression of prolactin-releasing peptide and prolactin in the euryhaline mudskippers (Periophthalmus modestus): prolactin-releasing peptide as a primary regulator of prolactin
J. Mol. Endocrinol., June 1, 2005; 34(3): 825 - 834.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. Grabauskas, S.-Y. Zhou, S. Das, Y. Lu, C. Owyang, and H. C Moises
Prolactin-releasing peptide affects gastric motor function in rat by modulating synaptic transmission in the dorsal vagal complex
J. Physiol., December 15, 2004; 561(3): 821 - 839.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Sahu
Minireview: A Hypothalamic Role in Energy Balance with Special Emphasis on Leptin
Endocrinology, June 1, 2004; 145(6): 2613 - 2620.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. B. Lawrence, Y.-L. Liu, M. J. Stock, and S. M. Luckman
Anorectic actions of prolactin-releasing peptide are mediated by corticotropin-releasing hormone receptors
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2004; 286(1): R101 - R107.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Engstrom, A. Brandt, S. Wurster, J.-M. Savola, and P. Panula
Prolactin Releasing Peptide Has High Affinity and Efficacy at Neuropeptide FF2 Receptors
J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 825 - 832.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Moriyama, T. Ito, A. Takahashi, M. Amano, S. A. Sower, T. Hirano, K. Yamamori, and H. Kawauchi
A Homolog of Mammalian PRL-Releasing Peptide (Fish Arginyl-Phenylalanyl-Amide Peptide) Is a Major Hypothalamic Peptide of PRL Release in Teleost Fish
Endocrinology, June 1, 2002; 143(6): 2071 - 2079.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Ozawa, M. Yamada, T. Satoh, T. Monden, K. Hashimoto, H. Kohga, Y. Hashiba, T. Sasaki, and M. Mori
Transcriptional Regulation of the Human PRL-Releasing Peptide (PrRP) Receptor Gene by a Dopamine 2 Receptor Agonist: Cloning and Characterization of the Human PrRP Receptor Gene and Its Promoter Region
Mol. Endocrinol., April 1, 2002; 16(4): 785 - 798.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. B. Lawrence, K. L. J. Ellacott, and S. M. Luckman
PRL-Releasing Peptide Reduces Food Intake and May Mediate Satiety Signaling
Endocrinology, February 1, 2002; 143(2): 360 - 367.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. L. J. Ellacott, C. B. Lawrence, N. J. Rothwell, and S. M. Luckman
PRL-Releasing Peptide Interacts with Leptin to Reduce Food Intake and Body Weight
Endocrinology, February 1, 2002; 143(2): 368 - 374.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. H. S. Lin, A. C. Arai, Z. Wang, H.-P. Nothacker, and O. Civelli
The Carboxyl Terminus of the Prolactin-Releasing Peptide Receptor Interacts with PDZ Domain Proteins Involved in alpha -Amino-3-hydroxy-5-methylisoxazole-4-propionic Acid Receptor Clustering
Mol. Pharmacol., November 1, 2001; 60(5): 916 - 923.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. J. Seal, C. J. Small, W. S. Dhillo, S. A. Stanley, C. R. Abbott, M. A. Ghatei, and S. R. Bloom
PRL-Releasing Peptide Inhibits Food Intake in Male Rats via the Dorsomedial Hypothalamic Nucleus and not the Paraventricular Hypothalamic Nucleus
Endocrinology, October 1, 2001; 142(10): 4236 - 4243.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Iijima, Y. Matsumoto, T. Yano, M. Tanaka, T. Yamamoto, K. Kakihara, Y. Kataoka, Y. Tamada, H. Matsumoto, N. Suzuki, et al.
A Novel Function of Prolactin-Releasing Peptide in the Control of Growth Hormone via Secretion of Somatostatin from the Hypothalamus
Endocrinology, July 1, 2001; 142(7): 3239 - 3243.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
T. Rubinek, M. Hadani, G. Barkai, S. Melmed, and I. Shimon
Prolactin (PRL)-Releasing Peptide Stimulates PRL Secretion from Human Fetal Pituitary Cultures and Growth Hormone Release from Cultured Pituitary Adenomas
J. Clin. Endocrinol. Metab., June 1, 2001; 86(6): 2826 - 2830.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Maruyama, H. Matsumoto, K. Fujiwara, J. Noguchi, C. Kitada, M. Fujino, and K. Inoue
Prolactin-Releasing Peptide as a Novel Stress Mediator in the Central Nervous System
Endocrinology, May 1, 2001; 142(5): 2032 - 2038.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
M. E. Freeman, B. Kanyicska, A. Lerant, and G. Nagy
Prolactin: Structure, Function, and Regulation of Secretion
Physiol Rev, October 1, 2000; 80(4): 1523 - 1631.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1999 by The Endocrine Society