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Selective Cloning of Cell Surface Proteins Involved in Organ Development: Epithelial Glycoprotein Is Involved in Normal Epithelial Differentiation

Departments of Protein Chemistry (J.-P.S., P.E.R., L.B., B.D., J.P.M.) and Molecular Biology (J.L., Q.G.), Genentech, Inc., South San Francisco, California 94080-4990

Address all correspondence and requests for reprints to: Dr. Jean-Philippe Stephan, Research Immunochemistry-AAT, Genentech, Inc., South San Francisco, California 94080-4990. E-mail: stephanj{at}gene.com

Coordinating the activities of neighboring cells during development in multicellular organisms requires complex cellular interactions involving secreted, cell surface, and extracellular matrix components. Although most cloning efforts have concentrated on secreted molecules, recent work has emphasized the importance of membrane-bound molecules during development. To identify developmental genes, we raised antibodies to normal embryonic pancreatic epithelial cell surface proteins. These antibodies were characterized and used to clone the genes coding for the proteins by a panning strategy. Using this approach, we cloned the rat homologue of the mouse epithelial glycoprotein (EGP). Our immunohistochemistry data, describing the expression of EGP during rat development, as well as our in vitro data, looking at the effect of the anti-EGP antibody and the extracellular domain of EGP on embryonic pancreatic epithelial cell number and volume, strongly suggest a role for EGP during pancreatic development.

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