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Orexin Receptors Are Expressed in the Adrenal Medulla of the Rat

Department of Physiology, Morphological Sciences (R.G., T.G.-C.) and Medicine (F.C.), Faculty of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain

Address all correspondence and requests for reprints to: Carlos Diéguez, M.D., Ph.D., University of Santiago de Compostela, Faculty of Medicine, Department of Physiology, Rua S. Francisco SN, Santiago de Compostela 15705, Spain.

Abstract

Two recently discovered hypothalamic peptides, orexin-A and orexin-B, play a role as mediators in the central mechanisms that regulate feeding behavior and sleep control. These peptides bind and activate two orexin receptors that belong to the G-protein coupled receptor superfamily. Morphological studies have detected mRNA expression of orexin receptors exclusively in the rat central nervous system. In this paper we demonstrate a strong level of expression of orexin receptor 1 and 2 in the adrenal medulla of the rat by RT-PCR immunohistochemistry. The results of the present study provide the first evidence showing that the adrenal medulla expresses orexin receptors, and thus appears to be a target tissue for orexins. This could open a new loop in which the central and autonomous nervous system may be involved in body weight homeostasis and sleep control.

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