help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ukena, K.
Right arrow Articles by Tsutsui, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ukena, K.
Right arrow Articles by Tsutsui, K.
Endocrinology Vol. 140, No. 2 805-813
Copyright © 1999 by The Endocrine Society

ARTICLES

Expression and Activity of 3ß-Hydroxysteroid Dehydrogenase/{Delta}5-{Delta}4-Isomerase in the Rat Purkinje Neuron during Neonatal Life1

Kazuyoshi Ukena, Chie Kohchi and Kazuyoshi Tsutsui

Laboratory of Brain Science (K.U., K.T.), Faculty of Integrated Arts and Sciences, and Radioisotope Center (C.K.), Hiroshima University, Higashi-Hiroshima 739-8521, Japan

Address all correspondence and requests for reprints to: Dr. Kazuyoshi Tsutsui, Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521, Japan. E-mail: tsutsui{at}ipc.hiroshima-u.ac.jp

Recently, we demonstrated that cytochrome P450 side-chain cleavage enzyme (P450scc) occurs in the rat cerebellar Purkinje cell after differentiation and remains during neonatal development and into adulthood. 3ß-Hydroxysteroid dehydrogenase/{Delta}5-{Delta}4-isomerase (3ßHSD) is also an essential enzyme for progesterone biosynthesis not only in peripheral steroidogenic glands but also in the nervous system. In the present study, therefore, the expression of 3ßHSD in the rat cerebellum was investigated during neonatal development and in the adult. RT-PCR analysis showed that the expression of 3ßHSD messenger RNA (mRNA) in the cerebellum was higher at 7–14 days of age than at other times. Biochemical studies together with HPLC analysis revealed that cerebellar slices at 10 days of age converted pregnenolone to progesterone, suggesting enzymatic activity of 3ßHSD. This conversion was significantly reduced by trilostane, a specific inhibitor of 3ßHSD. A specific RIA indicated that progesterone concentrations in the cerebellum were higher at 3 and 10 days of age than at 60 days of age. The progesterone level in the cerebellum was significantly higher than that in plasma at 10 days of age. In contrast, the concentrations in both cerebellum and plasma at 3 and 60 days of age were similar. In the present study, the site of 3ßHSD mRNA expression in the cerebellum was further examined in neonatal and adult rats using in situ hybridization. The cerebellar expression of 3ßHSD mRNA was obscure at 3 days of age, whereas intense expression occurred in Purkinje cells and external granule cells throughout the cerebellum at 10 days of age. 3ßHSD mRNA was also expressed in Purkinje cells and granule cells at 60 days of age, but a restricted expression was observed along the cerebellar meninges.

These results suggest that the steroidogenic enzyme 3ßHSD as well as P450scc are expressed at least in the cerebellar Purkinje cell. The expression of 3ßHSD, however, may increase for a limited period around 10 days of age, unlike P450scc.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
K. Tsutsui
Progesterone Biosynthesis and Action in the Developing Neuron
Endocrinology, June 1, 2008; 149(6): 2757 - 2761.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Mani
Progestin Receptor Subtypes in the Brain: The Known and the Unknown
Endocrinology, June 1, 2008; 149(6): 2750 - 2756.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Sasahara, H. Shikimi, S. Haraguchi, H. Sakamoto, S.-i. Honda, N. Harada, and K. Tsutsui
Mode of Action and Functional Significance of Estrogen-Inducing Dendritic Growth, Spinogenesis, and Synaptogenesis in the Developing Purkinje Cell
J. Neurosci., July 11, 2007; 27(28): 7408 - 7417.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Ubuka, G. E. Bentley, K. Ukena, J. C. Wingfield, and K. Tsutsui
Melatonin induces the expression of gonadotropin-inhibitory hormone in the avian brain
PNAS, February 22, 2005; 102(8): 3052 - 3057.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Matsunaga, K. Ukena, E.-E. Baulieu, and K. Tsutsui
7{alpha}-Hydroxypregnenolone acts as a neuronal activator to stimulate locomotor activity of breeding newts by means of the dopaminergic system
PNAS, December 7, 2004; 101(49): 17282 - 17287.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Sakamoto, Y. Mezaki, H. Shikimi, K. Ukena, and K. Tsutsui
Dendritic Growth and Spine Formation in Response to Estrogen in the Developing Purkinje Cell
Endocrinology, October 1, 2003; 144(10): 4466 - 4477.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Ibanez, R. Guennoun, P. Liere, B. Eychenne, A. Pianos, M. El-Etr, E.-E. Baulieu, and M. Schumacher
Developmental Expression of Genes Involved in Neurosteroidogenesis: 3{beta}-Hydroxysteroid Dehydrogenase/{Delta}5-{Delta}4 Isomerase in the Rat Brain
Endocrinology, July 1, 2003; 144(7): 2902 - 2911.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. R. King, P. R. Manna, T. Ishii, P. J. Syapin, S. D. Ginsberg, K. Wilson, L. P. Walsh, K. L. Parker, D. M. Stocco, R. G. Smith, et al.
An Essential Component in Steroid Synthesis, the Steroidogenic Acute Regulatory Protein, Is Expressed in Discrete Regions of the Brain
J. Neurosci., December 15, 2002; 22(24): 10613 - 10620.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Hiroi, W. Kishimoto, T. Chow, S. Imaoka, T. Igarashi, and Y. Funae
Progesterone Oxidation by Cytochrome P450 2D Isoforms in the Brain
Endocrinology, September 1, 2001; 142(9): 3901 - 3908.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Sakamoto, K. Ukena, and K. Tsutsui
Effects of Progesterone Synthesized De Novo in the Developing Purkinje Cell on Its Dendritic Growth and Synaptogenesis
J. Neurosci., August 15, 2001; 21(16): 6221 - 6232.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Kimoto, T. Tsurugizawa, Y. Ohta, J.'y. Makino, H.-o. Tamura, Y. Hojo, N. Takata, and S. Kawato
Neurosteroid Synthesis by Cytochrome P450-Containing Systems Localized in the Rat Brain Hippocampal Neurons: N-Methyl-D-Aspartate and Calcium-Dependent Synthesis
Endocrinology, August 1, 2001; 142(8): 3578 - 3589.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
E. Falkenstein, H.-C. Tillmann, M. Christ, M. Feuring, and M. Wehling
Multiple Actions of Steroid Hormones---A Focus on Rapid, Nongenomic Effects
Pharmacol. Rev., December 1, 2000; 52(4): 513 - 556.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Takase, K. Ukena, T. Yamazaki, S. Kominami, and K. Tsutsui
Pregnenolone, Pregnenolone Sulfate, and Cytochrome P450 Side-Chain Cleavage Enzyme in the Amphibian Brain and Their Seasonal Changes
Endocrinology, April 1, 1999; 140(4): 1936 - 1944.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1999 by The Endocrine Society