This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, J. J. Articles by Fazleabas, A. T. Search for Related Content PubMed PubMed Citation Articles by Kim, J. J. Articles by Fazleabas, A. T.

Insulin-Like Growth Factor Binding Protein-1 Expression in Baboon Endometrial Stromal Cells: Regulation by Filamentous Actin and Requirement for de Novo Protein Synthesis

Departments of Obstetrics and Gynecology (J.J.K., A.T.F.) and Physiology and Biophysics (R.C.J., A.T.F.), University of Illinois at Chicago, Chicago, Illinois 60212-7313

Address all correspondence and requests for reprints to: Dr. A. T. Fazleabas, Department of Obstetrics and Gynecology, University of Illinois at Chicago, 820 South Wood Street (M/C 808), Chicago, Illinois 60612. E-mail: asgi{at}uic.edu

Stromal fibroblasts in the primate endometrium undergo dramatic morphological and biochemical changes in response to pregnancy. This transformation is characterized by the expression of insulin-like growth factor binding protein-1 (IGFBP-1). Stromal cells from the baboon endometrium of nonpregnant animals were cultured and subsequently treated with cytochalasin D to disrupt actin filaments. In response to cytochalasin D treatment, cells contracted and became rounded as early as 10 min after the initiation of treatment. When cytochalasin D was removed, cells reverted back to their original fibroblastic shape within 1 h. After cells were treated with cytochalasin D for 5 h, addition of (Bu)₂cAMP and/or hormones (estradiol, medroxyprogesterone acetate, and relaxin) resulted in the expression of IGFBP-1 messenger RNA and protein within 24 h. Cells with an intact cytoskeleton did not express detectable levels of IGFBP-1 in response to hormones and/or (Bu)₂cAMP. Furthermore, the addition of cycloheximide inhibited expression of IGFBP-1 in cytochalasin D-treated cells. Stromal cells were also isolated from early pregnant and simulated pregnant animals. Within 48 h, cells from both the pregnant and simulated pregnant animals produced IGFBP-1 in response to hormones and/or (Bu)₂cAMP. In these studies, IGFBP-1 expression was also inhibited by cycloheximide. These studies suggest that induction of IGFBP-1 requires an intermediary protein and that alterations in the cytoskeleton may be involved.

This article has been cited by other articles:

				I. Ihnatovych, W. Hu, J. L. Martin, A. T. Fazleabas, P. de Lanerolle, and Z. Strakova Increased Phosphorylation of Myosin Light Chain Prevents in Vitro Decidualization Endocrinology, July 1, 2007; 148(7): 3176 - 3184. [Abstract] [Full Text] [PDF]

				P. Cameo, M. Szmidt, Z. Strakova, P. Mavrogianis, K. L. Sharpe-Timms, and A. T. Fazleabas Decidualization Regulates the Expression of the Endometrial Chorionic Gonadotropin Receptor in the Primate Biol Reprod, November 1, 2006; 75(5): 681 - 689. [Abstract] [Full Text] [PDF]

				A. Jasinska, Z. Strakova, M. Szmidt, and A. T. Fazleabas Human Chorionic Gonadotropin and Decidualization in Vitro Inhibits Cytochalasin-D-Induced Apoptosis in Cultured Endometrial Stromal Fibroblasts Endocrinology, September 1, 2006; 147(9): 4112 - 4121. [Abstract] [Full Text] [PDF]

				G. S. Daftary and H. S. Taylor Endocrine Regulation of HOX Genes Endocr. Rev., June 1, 2006; 27(4): 331 - 355. [Abstract] [Full Text] [PDF]

				J.J. Kim, O.L. Buzzio, S. Li, and Z. Lu Role of FOXO1A in the Regulation of Insulin-Like Growth Factor-Binding Protein-1 in Human Endometrial Cells: Interaction with Progesterone Receptor Biol Reprod, October 1, 2005; 73(4): 833 - 839. [Abstract] [Full Text] [PDF]

				A. Jasinska, V. Han, A. T. Fazleabas, and J. J. Kim Induction of Insulin-Like Growth Factor Binding Protein-1 Expression in Baboon Endometrial Stromal Cells by Cells of Trophoblast Origin Reproductive Sciences, September 1, 2004; 11(6): 399 - 405. [Abstract] [PDF]

				Z. Strakova, M. Szmidt, S. Srisuparp, and A. T. Fazleabas Inhibition of Matrix Metalloproteinases Prevents the Synthesis of Insulin-Like Growth Factor Binding Protein-1 during Decidualization in the Baboon Endocrinology, December 1, 2003; 144(12): 5339 - 5346. [Abstract] [Full Text] [PDF]

				G. A. Johnson, R. C. Burghardt, F. W. Bazer, and T. E. Spencer Osteopontin: Roles in Implantation and Placentation Biol Reprod, November 1, 2003; 69(5): 1458 - 1471. [Abstract] [Full Text] [PDF]

				T. Maruyama, Y. Yoshimura, and H. Sabe Tyrosine Phosphorylation and Subcellular Localization of Focal Adhesion Proteins during in Vitro Decidualization of Human Endometrial Stromal Cells Endocrinology, December 1, 1999; 140(12): 5982 - 5990. [Abstract] [Full Text]

				A. T. Fazleabas, K. M. Donnelly, S. Srinivasan, J. D. Fortman, and J. B. Miller Modulation of the baboon (Papio anubis) uterine endometrium by chorionic gonadotrophin during the period of uterine receptivity PNAS, March 2, 1999; 96(5): 2543 - 2548. [Abstract] [Full Text] [PDF]