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Insulin-Induced Up-Regulation of Lipoprotein Lipase Messenger Ribonucleic Acid and Activity in Mammary Gland¹

Faculty of Experimental and Technical Sciences (P.R., E.H.), Universidad San Pablo-CEU, E-28668 Madrid, Spain; and Department of Research (A.M.-H.), Hospital Ramón y Cajal, E-28034 Madrid, Spain

Address all correspondence and requests for reprints to: E. Herrera, Facultad de Ciencias Experimentales, Universidad San Pablo-CEU, Ctra. de Boadilla del Monte km 5.3, E-28668 Madrid, Spain. E-mail: e.herrera{at}offcampus.es

The effects of insulin on lipoprotein lipase (LPL) messenger RNA (mRNA) were studied in mammary glands from virgin and late-pregnant rats. Virgin and pregnant rats (at 17 days of gestation) were subjected to a continuous infusion (35 ml/day) with 50% glucose for 72 h to produce a prolonged hyperinsulinemic-euglycemic condition. Gestation causes a 4-fold increase in LPL mRNA accompanied by a 3- to 4-fold increase in total LPL activity. Experimental hyperinsulinemia, generated by the 50% glucose infusion, had a similar effect in both pregnant and virgin rats, by enhancing (2- to 3-fold) both their LPL activity and LPL mRNA. Thus, total LPL activity and LPL mRNA significantly correlated with plasma insulin (r = 0.789, P < 0.001; and r = 0.772, P < 0.001, respectively). Furthermore, total LPL activity was correlated with LPL mRNA (r = 0.765, P = 0.001). In conclusion, the present study shows that insulin participates in the control of LPL expression in mammary glands, revealing its role as a modulator of the enzyme at a mRNA level.

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