This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Machinal, F. Articles by Giudicelli, Y. Search for Related Content PubMed PubMed Citation Articles by Machinal, F. Articles by Giudicelli, Y.

In Vivo and in Vitro ob Gene Expression and Leptin Secretion in Rat Adipocytes: Evidence for a Regional Specific Regulation by Sex Steroid Hormones

Service de Biochimie, INSERM CJF 94–02, Faculté de Médecine Paris-Ouest, Université Descartes (ParisV) Centre Hospitalier de Poissy, 78303 Poissy Cedex France

Address all correspondence and requests for reprints to: Yves Giudicelli, Service de Biochimie, Centre Hospitalier, 78303 Poissy Cedex, France.

As a sexual dimorphism appears in plasma leptin levels, the aim of the present study was to investigate, in vivo and in vitro, the influence of sex steroid hormones on ob messenger RNA (mRNA) and leptin expressions in rat fat cells from various anatomical localizations. In male rats, castration resulted in a modulation of ob gene mRNA expression which was increased by 2-fold in perirenal and half-reduced in sc adipocytes. Moreover, in isolated fat cells from both perirenal and sc fat depots, ob gene mRNA expression was reduced by 20% after a 24-h in vitro exposure to dihydrotestosterone (10^-8 M). This effect of dihydrotestosterone on ob mRNA was prevented by exposure to the antiandrogen cyproterone acetate and also by actinomycin D. In contrast, leptin secretion from both perirenal and sc adipocytes was unchanged after 24 h exposure to dihydrotestosterone. In female rats, ovariectomy induced a 25% decrease in ob gene mRNA expression in perirenal fat cells. In vitro studies revealed that a 24-h exposure to 17-ß estradiol (10^-8 M) induced a 1.4-, 1.2-, and 1.75-fold increase in ob mRNA expression and a 3.8-, 1.65- and 2-fold increase in leptin secretion in sc, perirenal and parametrial adipocytes, respectively. Moreover, these effects were prevented by the antiestrogen ICI₁₈₂₇₈₀ and also by actinomycin D. Altogether, these results demonstrate that in rat adipocytes, estrogens, and androgens modulate ob gene expression at the mRNA level through sex steroid receptor-dependent transcriptional mechanisms.

This article has been cited by other articles:

				K. R. Belsito, B. M. Vester, T. Keel, T. K. Graves, and K. S. Swanson Impact of ovariohysterectomy and food intake on body composition, physical activity, and adipose gene expression in cats J Anim Sci, February 1, 2009; 87(2): 594 - 602. [Abstract] [Full Text] [PDF]

				W. Fan, T. Yanase, Y. Nishi, S. Chiba, T. Okabe, M. Nomura, H. Yoshimatsu, S. Kato, R. Takayanagi, and H. Nawata Functional Potentiation of Leptin-Signal Transducer and Activator of Transcription 3 Signaling by the Androgen Receptor Endocrinology, December 1, 2008; 149(12): 6028 - 6036. [Abstract] [Full Text] [PDF]

				A. Orgaard and L. Jensen The Effects of Soy Isoflavones on Obesity Experimental Biology and Medicine, September 1, 2008; 233(9): 1066 - 1080. [Abstract] [Full Text] [PDF]

				I-C. Yu, H.-Y. Lin, N.-C. Liu, R.-S. Wang, J. D. Sparks, S. Yeh, and C. Chang Hyperleptinemia without Obesity in Male Mice Lacking Androgen Receptor in Adipose Tissue Endocrinology, May 1, 2008; 149(5): 2361 - 2368. [Abstract] [Full Text] [PDF]

				E. Dos Santos, M.-N. Dieudonne, M.-C. Leneveu, R. Pecquery, V. Serazin, and Y. Giudicelli In vitro effects of chorionic gonadotropin hormone on human adipose development J. Endocrinol., August 1, 2007; 194(2): 313 - 325. [Abstract] [Full Text] [PDF]

				S. R. Thorn, M. J. Meyer, M. E. Van Amburgh, and Y. R. Boisclair Effect of Estrogen on Leptin and Expression of Leptin Receptor Transcripts in Prepubertal Dairy Heifers J Dairy Sci, August 1, 2007; 90(8): 3742 - 3750. [Abstract] [Full Text] [PDF]

				A.-M. Jaubert, N. Mehebik-Mojaat, D. Lacasa, D. Sabourault, Y. Giudicelli, and C. Ribiere Nongenomic Estrogen Effects on Nitric Oxide Synthase Activity in Rat Adipocytes Endocrinology, May 1, 2007; 148(5): 2444 - 2452. [Abstract] [Full Text] [PDF]

				A. Alonso, R. Fernandez, M. Moreno, P. Ordonez, F. Diaz, and C. Gonzalez Leptin and Its Receptor Are Controlled by 17{beta}-Estradiol in Peripheral Tissues of Ovariectomized Rats Experimental Biology and Medicine, April 1, 2007; 232(4): 542 - 549. [Abstract] [Full Text] [PDF]

				A M Lorincz and S Sukumar Molecular links between obesity and breast cancer. Endocr. Relat. Cancer, June 1, 2006; 13(2): 279 - 292. [Abstract] [Full Text] [PDF]

				D. J. Clegg, L. M. Brown, S. C. Woods, and S. C. Benoit Gonadal hormones determine sensitivity to central leptin and insulin. Diabetes, April 1, 2006; 55(4): 978 - 987. [Abstract] [Full Text] [PDF]

				M. C. Henson and V. D. Castracane Leptin in Pregnancy: An Update Biol Reprod, February 1, 2006; 74(2): 218 - 229. [Abstract] [Full Text] [PDF]

				M. Lappas, K. Yee, M. Permezel, and G. E Rice Release and regulation of leptin, resistin and adiponectin from human placenta, fetal membranes, and maternal adipose tissue and skeletal muscle from normal and gestational diabetes mellitus-complicated pregnancies J. Endocrinol., September 1, 2005; 186(3): 457 - 465. [Abstract] [Full Text] [PDF]

				M. Monjo, E. Pujol, and P. Roca {alpha}2- to {beta}3-Adrenoceptor switch in 3T3-L1 preadipocytes and adipocytes: modulation by testosterone, 17{beta}-estradiol, and progesterone Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E145 - E150. [Abstract] [Full Text] [PDF]

				H.-Y. Lin, Q. Xu, S. Yeh, R.-S. Wang, J. D. Sparks, and C. Chang Insulin and Leptin Resistance With Hyperleptinemia in Mice Lacking Androgen Receptor Diabetes, June 1, 2005; 54(6): 1717 - 1725. [Abstract] [Full Text] [PDF]

				J. D. Veldhuis, J. N. Roemmich, E. J. Richmond, A. D. Rogol, J. C. Lovejoy, M. Sheffield-Moore, N. Mauras, and C. Y. Bowers Endocrine Control of Body Composition in Infancy, Childhood, and Puberty Endocr. Rev., February 1, 2005; 26(1): 114 - 146. [Abstract] [Full Text] [PDF]

				V. Giusti, M. Suter, C. Verdumo, R. C. Gaillard, P. Burckhardt, and F. P. Pralong Molecular Determinants of Human Adipose Tissue: Differences between Visceral and Subcutaneous Compartments in Obese Women J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1379 - 1384. [Abstract] [Full Text] [PDF]

				M. R. Garcia, M. Amstalden, S. W. Williams, R. L. Stanko, C. D. Morrison, D. H. Keisler, S. E. Nizielski, and G. L. Williams Serum leptin and its adipose gene expression during pubertal development, the estrous cycle, and different seasons in cattle J Anim Sci, August 1, 2002; 80(8): 2158 - 2167. [Abstract] [Full Text] [PDF]

				J. G. Geisler, W. Zawalich, K. Zawalich, J. R.T. Lakey, H. Stukenbrok, A. J. Milici, and W. C. Soeller Estrogen Can Prevent or Reverse Obesity and Diabetes in Mice Expressing Human Islet Amyloid Polypeptide Diabetes, July 1, 2002; 51(7): 2158 - 2169. [Abstract] [Full Text] [PDF]

				F. Machinal-Quelin, M. N. Dieudonne, M. C. Leneveu, R. Pecquery, and Y. Giudicelli Proadipogenic effect of leptin on rat preadipocytes in vitro: activation of MAPK and STAT3 signaling pathways Am J Physiol Cell Physiol, April 1, 2002; 282(4): C853 - C863. [Abstract] [Full Text] [PDF]

				J. S. Torday, H. Sun, L. Wang, and E. Torres Pre- and Postnatal Lung Development, Maturation, and Plasticity: Leptin mediates the parathyroid hormone-related protein paracrine stimulation of fetal lung maturation Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L405 - L410. [Abstract] [Full Text] [PDF]

				P. Concannon, K. Levac, R. Rawson, B. Tennant, and A. Bensadoun Seasonal changes in serum leptin, food intake, and body weight in photoentrained woodchucks Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2001; 281(3): R951 - R959. [Abstract] [Full Text] [PDF]

				J. S. O'Neil, A. E. Green, D. E. Edwards, K. F. Swan, T. Gimpel, V. D. Castracane, and M. C. Henson Regulation of Leptin and Leptin Receptor in Baboon Pregnancy: Effects of Advancing Gestation and Fetectomy J. Clin. Endocrinol. Metab., June 1, 2001; 86(6): 2518 - 2524. [Abstract] [Full Text] [PDF]

				G. Fruhbeck, J. Gomez-Ambrosi, F. J. Muruzabal, and M. A. Burrell The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation Am J Physiol Endocrinol Metab, June 1, 2001; 280(6): E827 - E847. [Abstract] [Full Text] [PDF]

				N. Kronfeld-Schor, J. Zhao, B. A. Silvia, E. Bicer, P. T. Mathews, R. Urban, S. Zimmerman, T. H. Kunz, and E. P. Widmaier Steroid-Dependent Up-Regulation of Adipose Leptin Secretion In Vitro During Pregnancy in Mice Biol Reprod, July 1, 2000; 63(1): 274 - 280. [Abstract] [Full Text]

				A. M. Isidori, F. Strollo, M. Morè, M. Caprio, A. Aversa, C. Moretti, G. Frajese, G. Riondino, and A. Fabbri Leptin and Aging: Correlation with Endocrine Changes in Male and Female Healthy Adult Populations of Different Body Weights J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 1954 - 1962. [Abstract] [Full Text]