help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robinson, C. E.
Right arrow Articles by Gimble, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robinson, C. E.
Right arrow Articles by Gimble, J. M.
Endocrinology Vol. 140, No. 4 1586-1593
Copyright © 1999 by The Endocrine Society

ARTICLES

A Corepressor and Chicken Ovalbumin Upstream Promoter Transcriptional Factor Proteins Modulate Peroxisome Proliferator-Activated Receptor-{gamma}2/Retinoid X Receptor {alpha}-Activated Transcription from the Murine Lipoprotein Lipase Promoter1

Claudius E. Robinson, Xiying Wu2, Zafar Nawaz, Sergio A. Onãte3 and Jeffrey M. Gimble

Zoology Department, University of Oklahoma (C.E.R., J.M.G.), Norman, Oklahoma 73019; the Departments of Surgery (X.W., J.M.G.) and Pathology (J.M.G.), University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190; and the Department of Cell Biology, Baylor College of Medicine (Z.N., S.A.O.), Houston, Texas 77030

Address all correspondence and requests for reprints to: Jeffrey M. Gimble, M.D., Ph.D., Department of Surgery, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, Oklahoma 73190. E-mail: jeffrey-gimble{at}ouhsc.edu

Complex physiological stimuli differentially regulate the tissue-specific transcription of the lipoprotein lipase (LPL) gene. A conserved DNA recognition element (-171 to -149 bp) within the promoter functions as a transcriptional enhancer when bound by the peroxisome proliferator-activated receptor-{gamma}2 (PPAR{gamma}2)/retinoid X receptor {alpha} (RXR{alpha}) heterodimer, but serves as a transcriptional silencer in the presence of unidentified double and single stranded DNA-binding proteins. To address this apparent paradox, the current study examined the effect of two classes of candidate comodulatory proteins, COUP-TF (chicken ovalbumin upstream promoter transcriptional factor) and the corepressor SMRT (silencing mediator of retinoic acid receptor and thyroid receptor). The expression of COUP-TF was detected by Western and Northern blots in a preadipocyte 3T3-L1 cell model during periods corresponding to increased LPL transcription. Cotransfection of COUP-TF expression constructs in the renal epithelial 293T cell line significantly increased transcription from the LPL promoter in synergy with PPAR{gamma}2/RXR{alpha} heterodimers. The COUP-TFII (ARP-1) protein specifically bound the LPL PPAR recognition element in electromobility shift assays and interacted directly with the ligand-binding domain of PPAR{gamma} in pull-down experiments. In contrast, cotransfection of SMRT repressed PPAR{gamma}2/RXR{alpha}-mediated LPL transcription in the absence or presence of COUP-TFII (ARP-1). The interaction between PPAR{gamma}2 and SMRT localized to the receptor-interactive domain 2 (amino acids 1260–1495) of the SMRT protein based on cotransfection and pull-down assays. These in vitro data indicate that COUP-TF proteins and SMRT modulate PPAR{gamma}-mediated LPL transcription in the 293T cell line.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. J. Calonge, J. Seoane, and J. Massague
Opposite Smad and Chicken Ovalbumin Upstream Promoter Transcription Factor Inputs in the Regulation of the Collagen VII Gene Promoter by Transforming Growth Factor-{beta}
J. Biol. Chem., May 28, 2004; 279(22): 23759 - 23765.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Coutant, S. Agaugue, L. Perrin-Cocon, P. Andre, and V. Lotteau
Sensing Environmental Lipids by Dendritic Cell Modulates Its Function
J. Immunol., January 1, 2004; 172(1): 54 - 60.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Lin, S. K. Kolluri, G.-q. Chen, and X.-k. Zhang
Regulation of Retinoic Acid-induced Inhibition of AP-1 Activity by Orphan Receptor Chicken Ovalbumin Upstream Promoter-Transcription Factor
J. Biol. Chem., June 7, 2002; 277(24): 21414 - 21422.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. W. Eubank, E. Duplus, S. C. Williams, C. Forest, and E. G. Beale
Peroxisome Proliferator-activated Receptor gamma and Chicken Ovalbumin Upstream Promoter Transcription Factor II Negatively Regulate the Phosphoenolpyruvate Carboxykinase Promoter via a Common Element*
J. Biol. Chem., August 3, 2001; 276(32): 30561 - 30569.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1999 by The Endocrine Society