This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Phillips, I. D. Articles by McMillen, I. C. Search for Related Content PubMed PubMed Citation Articles by Phillips, I. D. Articles by McMillen, I. C.

The Regulation of Prolactin Receptor Messenger Ribonucleic Acid Levels in the Sheep Liver before Birth: Relative Roles of the Fetal Hypothalamus, Cortisol, and the External Photoperiod¹

Department of Physiology, University of Adelaide (I.D.P., D.C.H., I.C.M.), Adelaide, South Australia 5005, Australia; and Department of Physiology, Colorado State University (R.V.A.), Fort Collins, Colorado 80523

Address all correspondence and requests for reprints to: Dr. Ian Phillips, Department of Physiology, Monash University, Clayton, Victoria 3168, Australia. E-mail: ian.phillips{at}med.monash.edu.au

We have investigated the separate actions of hypothalamo-pituitary disconnection (HPD), with or without cortisol administration, and changes in the external photoperiod on the regulation of the levels of messenger RNA (mRNA) encoding long (PRLR1) and short (PRLR2) forms of PRL receptor in the liver of the fetal lamb. In pregnant Merino ewes (n = 20), the hypothalamus and pituitary were surgically disconnected in 13 fetuses (HPD group), and fetal vascular catheters were implanted in the HPD group and in an additional 7 fetuses (intact + saline group) between 104–120 days gestation (d). Fetal sheep in the HPD group were infused with either cortisol (3.5 mg/4.8 ml saline/24 h; HPD + F; n = 5) or saline for 5 days between 134–141 d, and saline was also infused in the intact group within the same gestational age range. A second group of pregnant ewes (n = 12) was kept in a 12-h light, 12-h dark cycle from 70 d until implantation of fetal vascular catheters between 106–120 d, after which ewes were allocated to either a long photoperiod (16 h of light, 8 h of darkness; LL group; n = 6) or a short photoperiod (8 h of light, 16 h of darkness; SL group; n = 6) regimen. Circulating cortisol concentrations were higher (P < 0.05) in the intact fetal sheep (18.7 ± 3.8 nmol/liter) than in the HPD + saline group (1.5 ± 0.6 nmol/liter), and were further increased (P < 0.05) in the HPD + cortisol group (97.4 ± 23.7 nmol/liter). Fetal PRL concentrations were lower (P < 0.05) in the HPD + saline (10.6 ± 4.3 ng/ml) and HPD ± cortisol (5.6 ± 2 ng/ml) groups compared with those in the intact group (38.9 ± 6.8 ng/ml). The levels of hepatic PRLR mRNA were higher (P < 0.05) in the intact (PRLR1, 27.4 ± 6.1; PRLR2, 17.7 ± 2.5) and HPD + cortisol (PRLR1, 23.4 ± 0.4; PRLR2, 15.3 ± 3.0) groups than in the HPD + saline group (PRLR1, 10.6 ± 1.8; PRLR2, 8.9 ± 1.8) at 140/141 d. The mean plasma PRL concentration in the LL group (70 ± 9 ng/ml) was higher (P < 0.05) than that in the SL group (34 ± 15 ng/ml), whereas the levels of hepatic PRLR1 mRNA (LL group, 4.6 ± 0.9; SL group, 4.3 ± 0.8) and PRLR2 mRNA (LL group, 3.4 ± 0.4; SL group, 3.0 ± 0.5) at 140–141 d were not different. These data indicate that cortisol acts directly or indirectly to maintain hepatic PRLR mRNA levels in the sheep fetus during late pregnancy. In contrast, changes in the external photoperiod and circulating PRL concentrations in the sheep fetus do not directly alter PRLR expression in the fetal liver. These studies provide further insight into the role that the PRL axis may play in the transduction of signals about the external environment to the fetus as it prepares for the transition to extrauterine life.

This article has been cited by other articles:

				S Pearce, H Budge, A Mostyn, E Genever, R Webb, P Ingleton, A M Walker, M E Symonds, and T Stephenson Prolactin, the prolactin receptor and uncoupling protein abundance and function in adipose tissue during development in young sheep J. Endocrinol., February 1, 2005; 184(2): 351 - 359. [Abstract] [Full Text] [PDF]

				A L Fowden and A J Forhead Endocrine mechanisms of intrauterine programming Reproduction, May 1, 2004; 127(5): 515 - 526. [Abstract] [Full Text] [PDF]

				M. Freemark Editorial: The Fetal Adrenal and the Maturation of the Growth Hormone and Prolactin Axes Endocrinology, May 1, 1999; 140(5): 1963 - 1965. [Full Text]