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Endocrinology Vol. 140, No. 5 2004-2009
Copyright © 1999 by The Endocrine Society

ARTICLES

17ß-Estradiol Decreases Nitric Oxide Synthase II Synthesis in Vascular Smooth Muscle Cells1

Valeria Zancan, Sabrina Santagati, Chiara Bolego, Elisabetta Vegeto, Adriana Maggi and Lina Puglisi

Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133 Milan, Italy

Address all correspondence and requests for reprints to: Lina Puglisi, Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133 Milan, Italy. E-mail: Lina.Puglisi{at}unimi.it

Several studies have provided evidence for a direct effect of 17ß-estradiol on vessel wall via interaction with the constitutively expressed nitric oxide synthase (NOS) by endothelium. The aim of the present study was to investigate the effect of 17ß-estradiol on inducible NOS (NOS II) in primary culture of smooth muscle cells (SMC) from rat aorta. We here prove that 17ß-estradiol decreases the content and activity of NOS II in SMC. This effect appears to be the consequence of ER activation, because: 1) ER{alpha} and ERß are expressed in rat aorta SMC grown in culture; 2) low concentrations of hormone modulate NOS II activity; 3) the specific ER{alpha} antagonist ICI182,780 completely blocks 17ß-estradiol effect. On the other hand, progesterone is deprived of any effect on NOS II content or activity, proving the specificity of 17ß-estradiol effect. In addition, we show that 17ß-estradiol can counteract the increase in NOS II activity following cytokine treatment. The observation could indicate a novel mechanism for the protective effects exerted by these hormones in cardiovascular diseases and atherosclerosis in particular.




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