This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, S. Articles by Segaloff, D. L. Search for Related Content PubMed PubMed Citation Articles by Chen, S. Articles by Segaloff, D. L.

Multiple Elements and Protein Factors Coordinate the Basal and Cyclic Adenosine 3',5'-Monophosphate-Induced Transcription of the Lutropin Receptor Gene in Rat Granulosa Cells¹

Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242

Address all correspondence and requests for reprints to: Deborah L. Segaloff, Ph.D., Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242. E-mail: deborah-segaloff{at}uiowa.edu

The expression of the lutropin receptor (LHR) in granulosa cells is a complex phenomenon under the hormonal control of FSH and estradiol. Using primary cultures of granulosa cells from immature female rats pretreated with diethylstilbestrol (a compound with estrogen-like activity), the role of FSH in LHR induction was studied. Previous studies from our laboratory have shown that FSH or 8-bromo-cAMP addition to these cells causes a marked increase in the rate of transcription of the rat LHR (rLHR) gene. The present studies were undertaken to compare the properties of the rLHR gene in undifferentiated vs. differentiated rat granulosa cells as a means of determining those elements that confer basal activity and cAMP responsiveness. Our studies show that the proximal 155 bp (relative to the translational initiation codon) of the 5'-flanking region of rLHR gene represent a minimal promoter that accounts for the basal expression of this receptor in rat granulosa cells. A major domain located between nucleotides (nt) -90 and -120, with another one possibly being between nt -120 and -155, induced activation of basal transcriptional activity. An inhibitory domain was observed to lie between nt -186 and -1375. Our data further show that multiple elements within the 2.1 kb of the 5'-flanking region of the rLHR gene are involved in the 8-bromo-cAMP-induced expression of the LHR gene. Of these, the three Sp1-binding sites within the proximal portion of the 5'-flanking region appear to be important for both basal as well as cAMP-induced rLHR gene transcription.

This article has been cited by other articles:

				A.-M. O'Carroll, S. J Lolait, and G. M Howell Transcriptional regulation of the rat apelin receptor gene: promoter cloning and identification of an Sp1 site necessary for promoter activity J. Mol. Endocrinol., February 1, 2006; 36(1): 221 - 235. [Abstract] [Full Text] [PDF]

				H. Youn, Y. Koo, I. Ji, and T. H. Ji An Upstream Initiator-Like Element Suppresses Transcription of the Rat Luteinizing Hormone Receptor Gene Mol. Endocrinol., May 1, 2005; 19(5): 1318 - 1328. [Abstract] [Full Text] [PDF]

				Y Kamei, Y Aoyama, T Fujimoto, N Kenmotsu, C Kishi, M Koushi, S Sugano, K Morohashi, R Kamiyama, and R Asakai A steroidogenic cell line with differentiation potential from mouse granulosa cells, transfected with Ad4BP and SV40 large T antigen genes J. Endocrinol., April 1, 2005; 185(1): 187 - 195. [Abstract] [Full Text] [PDF]

				T. Sekiguchi, T. Mizutani, K. Yamada, T. Yazawa, H. Kawata, M. Yoshino, T. Kajitani, T. Kameda, T. Minegishi, and K. Miyamoto Transcriptional Regulation of the Epiregulin Gene in the Rat Ovary Endocrinology, December 1, 2002; 143(12): 4718 - 4729. [Abstract] [Full Text] [PDF]

				T. Hamalainen, J. Kero, M. Poutanen, and I. Huhtaniemi Transgenic Mice Harboring Murine Luteinizing Hormone Receptor Promoter/{beta}-Galactosidase Fusion Genes: Different Structural and Hormonal Requirements of Expression in the Testis, Ovary, and Adrenal Gland Endocrinology, October 1, 2002; 143(10): 4096 - 4103. [Abstract] [Full Text] [PDF]

				M. Yoshino, T. Mizutani, K. Yamada, M. Tsuchiya, T. Minegishi, T. Yazawa, H. Kawata, T. Sekiguchi, T. Kajitani, and K. Miyamoto Early Growth Response Gene-1 Regulates the Expression of the Rat Luteinizing Hormone Receptor Gene Biol Reprod, June 1, 2002; 66(6): 1813 - 1819. [Abstract] [Full Text] [PDF]

				M. Ascoli, F. Fanelli, and D. L. Segaloff The Lutropin/Choriogonadotropin Receptor, A 2002 Perspective Endocr. Rev., April 1, 2002; 23(2): 141 - 174. [Abstract] [Full Text] [PDF]

				Y. Zhang and M. L. Dufau EAR2 and EAR3/COUP-TFI Regulate Transcription of the Rat LH Receptor Mol. Endocrinol., November 1, 2001; 15(11): 1891 - 1905. [Abstract] [Full Text] [PDF]

				T. Hamalainen, M. Poutanen, and I. Huhtaniemi Promoter Function of Different Lengths of the Murine Luteinizing Hormone Receptor Gene 5'-Flanking Region in Transfected Gonadal Cells and in Transgenic Mice Endocrinology, June 1, 2001; 142(6): 2427 - 2434. [Abstract] [Full Text] [PDF]

				S. Chen, X. Liu, and D. L. Segaloff Identification of an SAS (Sp1c Adjacent Site)-Like Element in the Distal 5'-Flanking Region of the Rat Lutropin Receptor Gene Essential for Cyclic Adenosine 3',5'-Monophosphate Responsiveness Endocrinology, May 1, 2001; 142(5): 2013 - 2021. [Abstract] [Full Text]

				S. Chen, X. Liu, and D. L. Segaloff A Novel Cyclic Adenosine 3',5'-Monophosphate-Responsive Element Involved In the Transcriptional Regulation of the Lutropin Receptor Gene in Granulosa Cells Mol. Endocrinol., September 1, 2000; 14(9): 1498 - 1508. [Abstract] [Full Text]