help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lanuza, G. M.
Right arrow Articles by Campo, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lanuza, G. M.
Right arrow Articles by Campo, S.
Endocrinology Vol. 140, No. 6 2549-2554
Copyright © 1999 by The Endocrine Society

ARTICLES

Dimeric Inhibin A and B Production Are Differentially Regulated by Hormones and Local Factors in Rat Granulosa Cells1

Guillermo M. Lanuza, Nigel P. Groome, J. Lino Barañao2 and Stella Campo2

Instituto de Biología Experimental (G.M.L., J.L.B.), CONICET and Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Argentina; School of Biological and Molecular Sciences (N.P.G.), Oxford Brookes University, Oxford OX3 OBP, United Kingdom; and Centro de Investigaciones Endocrinológicas (S.C.), Hospital de Niños "Ricardo Gutierrez", Buenos Aires, 1425 Argentina

Address all correspondence and requests for reprints to: J. Lino Barañao, Ph.D., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina. E-mail: lbaranao{at}dna.uba.ar

In the present study, we have examined the role of hormones and growth factors in regulating dimeric inhibin production in immature rat granulosa cells. Purified granulosa cells from estrogen-primed immature rats were cultured under defined conditions. Inhibins A and B in the culture media were measured using a two-site enzyme-linked immunosorbent assay specific for each dimer. Under basal conditions, granulosa cells produced 14-fold more inhibin A than inhibin B (inhibin A, 2.0; inhibin B, 0.14 ng/ml, measured against human standards; average A/B apparent ratio, 14). Addition of increasing doses of FSH elicited dose-dependent increases in both inhibins, the effects being more pronounced on inhibin A than on inhibin B (9.4- and 4.1-fold increases, respectively; average A/B ratio, 34). Estradiol, when added alone, stimulated inhibin A production 3- to 6-fold, whereas minor changes were observed in inhibin B production. Insulin-like growth factor-I produced a similar stimulation of both inhibins (3-fold stimulation over control). This growth factor, however, induced a marked dissociation in the sensitivity of inhibins A and B to FSH stimulation, with maximal stimulation of inhibin B observed at comparatively lower concentrations of the gonadotropin. Transforming growth factor-ß (TGF-ß, 5 ng/ml) had a more marked stimulatory effect on inhibin B than on inhibin A production (7- to 14-fold vs. 2- to 5-fold for inhibin B and A, respectively). A more pronounced differential stimulation of inhibin B was also exerted by another member of the TGF-ß superfamily, activin A (A/B ratio, 0.66). This preferential stimulation of inhibin B by TGF-ß and activin A was amplified in the presence of FSH. Coculture of rat granulosa cells with freshly isolated bovine oocytes was also associated with a marked stimulation of inhibin B production (100-fold increase) and a comparatively lower stimulation of inhibin A (10-fold increase; A/B ratio, 1). The discrepancy between the proportion of inhibin dimers in serum (A/B ratio, 0.13) and those produced by untreated granulosa cells may suggest that intraovarian factors, such as TGF-ß, activin A, or oocyte-derived factor(s), are responsible for the shift of the ratio toward the predominance of inhibin B.




This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
R. B. Gilchrist, M. Lane, and J. G. Thompson
Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality
Hum. Reprod. Update, March 1, 2008; 14(2): 159 - 177.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. B. Gilchrist, L. J. Ritter, S. Myllymaa, N. Kaivo-Oja, R. A. Dragovic, T. E. Hickey, O. Ritvos, and D. G. Mottershead
Molecular basis of oocyte-paracrine signalling that promotes granulosa cell proliferation
J. Cell Sci., September 15, 2006; 119(18): 3811 - 3821.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
M Anttonen, H Parviainen, A Kyronlahti, M Bielinska, D B Wilson, O Ritvos, and M Heikinheimo
GATA-4 is a granulosa cell factor employed in inhibin-{alpha} activation by the TGF-{beta} pathway.
J. Mol. Endocrinol., June 1, 2006; 36(3): 557 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E.V. Velasquez, R.V. Trigo, S. Creus, S. Campo, and H.B. Croxatto
Pituitary-ovarian axis during lactational amenorrhoea. I. Longitudinal assessment of follicular growth, gonadotrophins, sex steroids and inhibin levels before and after recovery of menstrual cyclicity
Hum. Reprod., April 1, 2006; 21(4): 909 - 915.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Omori, K. Nakamura, S. Yamashita, H. Matsuda, T. Mizutani, K. Miyamoto, and T. Minegishi
Effect of Follicle-Stimulating Hormone and Estrogen on the Expression of Betaglycan Messenger Ribonucleic Acid Levels in Cultured Rat Granulosa Cells
Endocrinology, August 1, 2005; 146(8): 3379 - 3386.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. A. Dragovic, L. J. Ritter, S. J. Schulz, F. Amato, D. T. Armstrong, and R. B. Gilchrist
Role of Oocyte-Secreted Growth Differentiation Factor 9 in the Regulation of Mouse Cumulus Expansion
Endocrinology, June 1, 2005; 146(6): 2798 - 2806.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Park, E. T. Maizels, Z. J. Feiger, H. Alam, C. A. Peters, T. K. Woodruff, T. G. Unterman, E. J. Lee, J. L. Jameson, and M. Hunzicker-Dunn
Induction of Cyclin D2 in Rat Granulosa Cells Requires FSH-dependent Relief from FOXO1 Repression Coupled with Positive Signals from Smad
J. Biol. Chem., March 11, 2005; 280(10): 9135 - 9148.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R.B. Gilchrist, L.J. Ritter, M. Cranfield, L.A. Jeffery, F. Amato, S.J. Scott, S. Myllymaa, N. Kaivo-Oja, H. Lankinen, D.G. Mottershead, et al.
Immunoneutralization of Growth Differentiation Factor 9 Reveals It Partially Accounts for Mouse Oocyte Mitogenic Activity
Biol Reprod, September 1, 2004; 71(3): 732 - 739.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. L. Loveland, M. Bakker, T. Meehan, E. Christy, V. von Schonfeldt, A. Drummond, and D. de Kretser
Expression of Bambi Is Widespread in Juvenile and Adult Rat Tissues and Is Regulated in Male Germ Cells
Endocrinology, September 1, 2003; 144(9): 4180 - 4186.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
F. Jimenez-Krassel, M. E. Winn, D. Burns, J. L. H. Ireland, and J. J. Ireland
Evidence for a Negative Intrafollicular Role for Inhibin in Regulation of Estradiol Production by Granulosa Cells
Endocrinology, May 1, 2003; 144(5): 1876 - 1886.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
N. Kaivo-Oja, J. Bondestam, M. Kamarainen, J. Koskimies, U. Vitt, M. Cranfield, K. Vuojolainen, J. P. Kallio, V. M. Olkkonen, M. Hayashi, et al.
Growth Differentiation Factor-9 Induces Smad2 Activation and Inhibin B Production in Cultured Human Granulosa-Luteal Cells
J. Clin. Endocrinol. Metab., February 1, 2003; 88(2): 755 - 762.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J.-S. Roh, J. Bondestam, S. Mazerbourg, N. Kaivo-Oja, N. Groome, O. Ritvos, and A. J. W. Hsueh
Growth Differentiation Factor-9 Stimulates Inhibin Production and Activates Smad2 in Cultured Rat Granulosa Cells
Endocrinology, January 1, 2003; 144(1): 172 - 178.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. M. Vitale, O. M. Gonzalez, F. Parborell, G. Irusta, S. Campo, and M. Tesone
Inhibin A Increases Apoptosis in Early Ovarian Antral Follicles of Diethylstilbestrol-Treated Rats
Biol Reprod, December 1, 2002; 67(6): 1989 - 1995.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. Jaatinen, J. Bondestam, T. Raivio, K. Hilden, L. Dunkel, N. Groome, and O. Ritvos
Activation of the Bone Morphogenetic Protein Signaling Pathway Induces Inhibin {beta}B-Subunit mRNA and Secreted Inhibin B Levels in Cultured Human Granulosa-Luteal Cells
J. Clin. Endocrinol. Metab., March 1, 2002; 87(3): 1254 - 1261.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. B. Herath, M. Yamashita, G. Watanabe, W. Jin, S. Tangtrongsup, A. Kojima, N. P. Groome, A. K. Suzuki, and K. Taya
Regulation of Follicle-Stimulating Hormone Secretion by Estradiol and Dimeric Inhibins in the Infantile Female Rat
Biol Reprod, December 1, 2001; 65(6): 1623 - 1633.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
T. Fujiwara, Y. Sidis, C. Welt, G. Lambert-Messerlian, J. Fox, A. Taylor, and A. Schneyer
Dynamics of Inhibin Subunit and Follistatin mRNA during Development of Normal and Polycystic Ovary Syndrome Follicles
J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4206 - 4215.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
C. K. Welt, Z. A. Smith, D. K. Pauler, and J. E. Hall
Differential Regulation of Inhibin A and Inhibin B by Luteinizing Hormone, Follicle-Stimulating Hormone, and Stage of Follicle Development
J. Clin. Endocrinol. Metab., June 1, 2001; 86(6): 2531 - 2537.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. Liu, C. Hyden-Granskog, and R. Voutilainen
Gonadotrophins inhibit and activin induces expression of inhibin/activin {beta}B subunit mRNA in cultured human granulosa-luteal cells
Mol. Hum. Reprod., April 1, 2001; 7(4): 319 - 323.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
C. K. Welt and A. L. Schneyer
Differential Regulation of Inhibin B and Inhibin A by Follicle-Stimulating Hormone and Local Growth Factors in Human Granulosa Cells from Small Antral Follicles
J. Clin. Endocrinol. Metab., January 1, 2001; 86(1): 330 - 336.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
R. Li, R. J. Norman, D. T. Armstrong, and R. B. Gilchrist
Oocyte-Secreted Factor(s) Determine Functional Differences Between Bovine Mural Granulosa Cells and Cumulus Cells
Biol Reprod, September 1, 2000; 63(3): 839 - 845.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
T. M. Pierson, Y. Wang, F. J. DeMayo, M. M. Matzuk, S. Y. Tsai, and B. W. O’Malley
Regulable Expression of Inhibin A in Wild-Type and Inhibin {alpha} Null Mice
Mol. Endocrinol., July 1, 2000; 14(7): 1075 - 1085.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1999 by The Endocrine Society