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Characterization and Localization of ß₂-Adrenergic Receptors in the Bovine Oviduct: Indication for Progesterone-Mediated Expression¹

Institute of Physiology, Technical University Munich-Weihenstephan (R.E., C.G., A.K.), Freising, D-85350 Germany; and the Department of Zoology II, University of Karlsruhe (W.K.), D-76128 Karlsruhe, Germany

Address all correspondence and requests for reprints to: Dr. R. Einspanier, Institute of Physiology, Technical University Munich, Weihenstephaner Berg 5, D-85350 Freising, Germany.

ß₂-Adrenergic receptors were detected in bovine oviductal epithelium by use of receptor binding studies and expression analysis. Complementary DNA cloning gave use to the first full-length bovine ß₂-adrenoceptor messenger RNA sequence (2030 bases). Receptor bioactivity in oviduct epithelial cells was characterized by specific ligand interaction and consequent cAMP generation. Expression studies demonstrated an estrous cycle-dependent regulation, with higher transcript levels and significantly increased binding capacity during the luteal phase. After progesterone supplementation, oviduct epithelial cells showed elevated receptor expression in culture, supporting the hypothesis that progesterone up-regulates the ß₂-adrenergic receptor within these cells. It seems likely that catecholamines from the circulation or from innervation might be able to influence reproductive success by regulating oviductal secretion.

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