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Endocrinology Vol. 140, No. 7 2968-2975
Copyright © 1999 by The Endocrine Society

ARTICLES

Expression of Peroxisome Proliferator-Activated Receptor {alpha} Messenger Ribonucleic Acid and Protein in Human and Rat Testis

Rüdiger Schultz, Wei Yan, Jorma Toppari, Alfred Völkl, Jan-Åke Gustafsson and Markku Pelto-Huikko

Tampere University Hospital (R.S.), Division of Pediatrics, Tampere, Finland; Institut f. Anatomie und Zellbiologie II Heidelberg (A.V.), Germany; Departments of Pediatrics and Physiology (W.Y., J.T.), University of Turku, Finland; Department of Medical Nutrition, Huddinge (J.-Å.G.), Sweden; Department of Developmental Biology, Medical School (M.P-H.), University of Tampere and Tampere University Hospital (M.-P.H.), Department of Pathology, Finland

Address all correspondence and requests for reprints to: Dr. M. Pelto-Huikko, Medical School, University of Tampere, P.O. Box 607, 33101 Tampere, Finland. E-mail: blmapel{at}poph.uta.fi

Peroxisome proliferator-activated receptor {alpha} (PPAR{alpha}), a member of the steroid hormone receptor superfamily, has been linked to lipid homeostasis and tumorigenesis in tissues with high expression of receptor protein. On the other hand, the role of PPAR{alpha} in tissues with a lower expression is not well known. Here we demonstrate the localization of PPAR{alpha} messenger RNA (mRNA) and protein in developing and adult rat testis. Additionally, we demonstrate the expression of PPAR{alpha} protein in adult human testis. Our experiments with Northern analysis, in situ hybridization and immunocytochemistry reveal a complex distribution of PPAR{alpha} in tubular and interstitial cells of both adult and developing rat testis. The overall expression is rather low but may be modified by exogenous or endogenous stimuli. An up-regulation of PPAR{alpha} mRNA could be observed after stimulation with FSH. In the developing rat testis, a clear expression of PPAR{alpha} mRNA was present from the first days after birth. Additionally, PPAR{alpha} mRNA and protein increased toward adulthood. In adult human testis PPAR{alpha} immunoreactivity (IR) was present in interstitial Leydig cells and tubular cells. In the seminiferous epithelium of adult human testis the expression of PPAR{alpha}-IR could be seen in meiotic spermatocytes, spermatids and myoid peritubular cells. The findings of our study suggest that PPAR{alpha} may be involved in the regulation of growth and differentiation of tubular and interstitial cells in rat and human testis.




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