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Endocrinology Vol. 140, No. 7 2983-2990
Copyright © 1999 by The Endocrine Society

ARTICLES

Transient Expression of c-erbAß1 Messenger Ribonucleic Acid and ß1 Thyroid Hormone Receptor Early in Adipogenesis of Ob 17 Cells1

Alexandra Dace, Gaiane Sarkissian, Laetitia Schneider, Claire Martin-El Yazidi, Jeannine Bonne, Alain Margotat, Richard Planells and Janine Torresani

INSERM U-476 et IFR 35, Université de la Méditerranée, Faculté de Médecine, 13385 Marseille Cedex 5, France

Address all correspondence and requests for reprints to: Dr. Janine Torresani, INSERM U-476, Faculté de Médecine, 27 boulevard Jean-Moulin, 13385 Marseille Cedex 5, France. E-mail: Janine.Torresani{at}medecine.univ-mrs.fr

In the murine Ob 17 preadipocyte cell line, the thyroid hormone T3 is an adipogenic factor necessary at an early stage for differentiation into adipocyte. We demonstrate here that this T3 dependence may involve a transient expression (at both the messenger RNA and the protein levels) of c-ErbA ß-type receptors (T3R), although a large body of T3R remained the product of the c-erbA{alpha} gene, as previously described. c-ErbAß1 (and not ß2) expression emerged significantly at growth arrest, peaked 2 days later, and almost disappeared in maturing adipocytes. This expression is related to the presence of T3, as total deprivation of culture medium from T3 prevented it, and the addition of 1.5 nM T3 to preconfluent cultures was able to restore it. When cells were cultured in the presence of T3 and thus were able to differentiate, the c-erbAß peak was accompanied by sequential rapid increases in CAAT/enhancer-binding protein-{delta} (C/EBP{delta}), peroxisome proliferator-activated-{gamma} receptor (PPAR{gamma}), and C/EBP{alpha} gene expressions. On the contrary, under thyroid hormone-deprived culture conditions that result in nondifferentiation of the preadipocytes, c-erbAß1, PPAR{gamma}, and the large C/EBP{alpha} expressions were blunted, and a moderate early increase in c-erbA{alpha}1 transcripts was sustained for a longer period. Addition of T3 to T3-deprived preconfluent cells restored PPAR{gamma} and C/EBP{alpha} expressions. Taken together, the results highlight the important role of T3 in the adipogenesis of Ob 17 cells through the involvement of both ß1 and {alpha}1 T3R subtypes.




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L. Schneider, C. El-Yazidi, A. Dace, M. Maraninchi, R. Planells, A. Margotat, and J. Torresani
Expression of the 1,25-(OH)2 vitamin D3 receptor gene during the differentiation of mouse Ob17 preadipocytes and cross talk with the thyroid hormone receptor signalling pathway
J. Mol. Endocrinol., February 1, 2005; 34(1): 221 - 235.
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Copyright © 1999 by The Endocrine Society