help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jonsson, C. K.
Right arrow Articles by Söder, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jonsson, C. K.
Right arrow Articles by Söder, O.
Endocrinology Vol. 140, No. 8 3755-3761
Copyright © 1999 by The Endocrine Society

ARTICLES

Constitutive Expression of Interleukin-1{alpha} Messenger Ribonucleic Acid in Rat Sertoli Cells Is Dependent upon Interaction with Germ Cells1

Cecilia K. Jonsson, Rolf H. Zetterström, Mikael Holst, Martti Parvinen and Olle Söder

Department of Woman and Child Health (C.K.J., M.H., O.S.), Astrid Lindgren Children’s Hospital, Pediatric Endocrinology Unit, Karolinska Hospital Q2:08, SE-17176 Stockholm, Sweden; Department of Neuroscience (R.H.Z.), Karolinska Institute, SE-17177 Stockholm, Sweden; and Department of Anatomy (M.P.), University of Turku, FIN-20520 Turku, Finland

Address all correspondence and requests for reprints to: Cecilia Jonsson, M.D., Department of Woman and Child Health, Astrid Lindgren Children’s Hospital, Pediatric Endocrinology Unit, Karolinska Institute & Hospital Q2:08, SE-17176 Stockholm, Sweden. E-mail cecilia.jonsson{at}kbh.ki.se

Interleukin-1 (IL-1), a proinflammatory cytokine originally isolated as a product of activated mononuclear phagocytes, consists of two distinct agonist proteins, IL-1{alpha} and IL-1ß, of which IL-1ß is the major inducible IL-1 protein produced by macrophages. We show here that mRNA of IL-1{alpha}, but not IL-1ß, is constitutively expressed by the intact rat testis and localize the transcript to Sertoli cells as confirmed by a novel squash technique. The expression is developmentally regulated and appears only after postnatal day 20 in the rat testis, corresponding to onset of puberty. IL-1{alpha} mRNA shows a stage-dependent expression pattern during the cycle of the seminiferous epithelium. It is low or absent in stage VII, but present in all other stages of the cycle. The same stage-dependent distribution was also observed at the protein level when bioactive IL-1 was measured in extracts of accurately defined one millimeter segments of seminiferous tubules. No IL-1{alpha} mRNA was detected in adult rat testes after germ cell depletion by fetal irradiation or cytostatic drug treatment. Because stage VII is the only segment of the seminiferous tubules lacking DNA replication, we propose that IL-1{alpha} is involved in this event during mitosis and meiosis of spermatogenesis and that its expression is dependent upon interactions between Sertoli cells and germ cells.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. H. Abel, P. J. Baker, H. M. Charlton, A. Monteiro, G. Verhoeven, K. De Gendt, F. Guillou, and P. J. O'Shaughnessy
Spermatogenesis and Sertoli Cell Activity in Mice Lacking Sertoli Cell Receptors for Follicle-Stimulating Hormone and Androgen
Endocrinology, July 1, 2008; 149(7): 3279 - 3285.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
P J O'Shaughnessy, L Hu, and P J Baker
Effect of germ cell depletion on levels of specific mRNA transcripts in mouse Sertoli cells and Leydig cells
Reproduction, June 1, 2008; 135(6): 839 - 850.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. S. Tash, R. Chakrasali, S. R. Jakkaraj, J. Hughes, S. K. Smith, K. Hornbaker, L. L. Heckert, S. B. Ozturk, M. K. Hadden, T. G. Kinzy, et al.
Gamendazole, an Orally Active Indazole Carboxylic Acid Male Contraceptive Agent, Targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and Stimulates Il1a Transcription in Rat Sertoli Cells
Biol Reprod, June 1, 2008; 78(6): 1139 - 1152.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. K. Zetterstrom, M.-L. Strand, and O. Soder
The high mobility group box chromosomal protein 1 is expressed in the human and rat testis where it may function as an antibacterial factor
Hum. Reprod., November 1, 2006; 21(11): 2801 - 2809.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
Y Okuma, A E O'Connor, T Hayashi, K L Loveland, D M de Kretser, and M P Hedger
Regulated production of activin A and inhibin B throughout the cycle of the seminiferous epithelium in the rat.
J. Endocrinol., August 1, 2006; 190(2): 331 - 340.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
N Renlund, Y Jo, I Svechnikova, M Holst, D M Stocco, O Soder, and K Svechnikov
Induction of steroidogenesis in immature rat Leydig cells by interleukin-1alpha is dependent on extracellular signal-regulated kinases.
J. Mol. Endocrinol., April 1, 2006; 36(2): 327 - 336.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Ishikawa, K. Hwang, D. Lazzarino, and P. L. Morris
Sertoli Cell Expression of Steroidogenic Acute Regulatory Protein-Related Lipid Transfer 1 and 5 Domain-Containing Proteins and Sterol Regulatory Element Binding Protein-1 Are Interleukin-1{beta} Regulated by Activation of c-Jun N-Terminal Kinase and Cyclooxygenase-2 and Cytokine Induction
Endocrinology, December 1, 2005; 146(12): 5100 - 5111.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
Y Okuma, A E O'Connor, J A Muir, P G Stanton, D M de Kretser, and M P Hedger
Regulation of activin A and inhibin B secretion by inflammatory mediators in adult rat Sertoli cell cultures
J. Endocrinol., October 1, 2005; 187(1): 125 - 134.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. Colon, K. V. Svechnikov, C. Carlsson-Skwirut, P. Bang, and O. Soder
Stimulation of Steroidogenesis in Immature Rat Leydig Cells Evoked by Interleukin-1{alpha} Is Potentiated by Growth Hormone and Insulin-Like Growth Factors
Endocrinology, January 1, 2005; 146(1): 221 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
I. Shiratori, M. Matsumoto, S. Tsuji, M. Nomura, K. Toyoshima, and T. Seya
Molecular cloning and functional characterization of guinea pig IL-12
Int. Immunol., September 1, 2001; 13(9): 1129 - 1139.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Maiti, M. L. Meistrich, G. Wilson, G. Shetty, M. Marcelli, M. J. McPhaul, P. L. Morris, and M. F. Wilkinson
Irradiation Selectively Inhibits Expression from the Androgen-Dependent Pem Homeobox Gene Promoter in Sertoli Cells
Endocrinology, April 1, 2001; 142(4): 1567 - 1577.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
T. Sultana, K. Svechnikov, G. Weber, and O. Soder
Molecular Cloning and Expression of a Functionally Different Alternative Splice Variant of Prointerleukin-1{{alpha}} from the Rat Testis
Endocrinology, December 1, 2000; 141(12): 4413 - 4418.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Aubry, C. Habasque, A.-P. Satie, B. Jégou, and M. Samson
Expression and Regulation of the CC-Chemokine Monocyte Chemoattractant Protein-1 in Rat Testicular Cells in Primary Culture
Biol Reprod, May 1, 2000; 62(5): 1427 - 1435.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1999 by The Endocrine Society