| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160
Address all correspondence and requests for reprints to: Dr. Michael J. Soares, Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160. E-mail: msoares{at}kumc.edu
The decidual/trophoblast PRL-related protein (d/tPRP) is dually expressed by decidual and trophoblast cells during pregnancy. We have characterized the proximal d/tPRP promoter responsible for directing d/tPRP expression in decidual and trophoblast cells. We have demonstrated that the proximal 93 bp of d/tPRP 5'-flanking DNA are sufficient to direct luciferase gene expression in primary decidual and Rcho-1 trophoblast cells, but not in fibroblast, undifferentiated uterine stromal cells or trophoblast cells of a labyrinthine lineage. The 93-bp d/tPRP promoter was also sufficient to direct differentiation-dependent expression in trophoblast giant cells. Mutational analysis demonstrated the differential importance of activating protein-1 and Ets regulatory elements (located within the proximal 93 bp of d/tPRP 5'-flanking DNA) for activation of the d/tPRP promoter in decidual vs. trophoblast cells. Disruption of the activating protein-1 regulatory element inhibited d/tPRP promoter activity by more than 95% in decidual cells, and approximately 80% trophoblast cells. Disruption of the Ets regulatory element reduced d/tPRP promoter activity by approximately 50% in decidual cells, while inactivating the d/tPRP promoter in trophoblast cells. Protein interactions with the trophoblast Ets regulatory element were shown to be cell type specific and to change during trophoblast giant cell formation. In conclusion, a 93-bp region of the d/tPRP promoter is shown to contain regulatory elements sufficient for gene activation in decidual and trophoblast cells.
This article has been cited by other articles:
 |
|
 |
|
  D. Ghosh, S. Sachdev, M. Hannink, and R. M. Roberts Coordinate Regulation of Basal and Cyclic 5'-Adenosine Monophosphate (cAMP)-Activated Expression of Human Chorionic Gonadotropin-{alpha} by Ets-2 and cAMP-Responsive Element Binding Protein Mol. Endocrinol., April 1, 2005; 19(4): 1049 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Rider, T Potapova, G Dai, and M J Soares Stimulation of a rat uterine stromal cell line in culture reveals a molecular switch for endocrine-dependent differentiation J. Endocrinol., January 1, 2005; 184(1): 119 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ezashi and R. M. Roberts Regulation of Interferon-{tau} (IFN-{tau}) Gene Promoters by Growth Factors that Target the Ets-2 Composite Enhancer: A Possible Model for Maternal Control of IFN-{tau} Production by the Conceptus during Early Pregnancy Endocrinology, October 1, 2004; 145(10): 4452 - 4460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ghosh, T. Ezashi, M. C. Ostrowski, and R. M. Roberts A Central Role for Ets-2 in the Transcriptional Regulation and Cyclic Adenosine 5'-Monophosphate Responsiveness of the Human Chorionic Gonadotropin-{beta} Subunit Gene Mol. Endocrinol., January 1, 2003; 17(1): 11 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamei, S. R. Jones, B. M. Chapman, K. L. MCGonigle, G. Dai, and M. J. Soares The Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Modulates the Endocrine Differentiation of Trophoblast Cells Mol. Endocrinol., July 1, 2002; 16(7): 1469 - 1481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ezashi, D. Ghosh, and R. M. Roberts Repression of Ets-2-Induced Transactivation of the Tau Interferon Promoter by Oct-4 Mol. Cell. Biol., December 1, 2001; 21(23): 7883 - 7891. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
