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Endocrinology Vol. 141, No. 1 375-384
Copyright © 2000 by The Endocrine Society

ARTICLES

Localization of Glucokinase-Like Immunoreactivity in the Rat Lower Brain Stem: For Possible Location of Brain Glucose-Sensing Mechanisms1

Fumihiko Maekawa, Yukiyasu Toyoda, Norihiro Torii, Ichitomo Miwa, Robert C. Thompson, Douglas L. Foster, Shinji Tsukahara, Hiroko Tsukamura and Kei-ichiro Maeda

Laboratory of Animal Reproduction, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan; the Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University (Y.T., N.T., I.M.), Nagoya 468-8503, Japan; and the Reproductive Sciences Program (R.C.T., D.L.F.) and the Departments of Obstetrics and Gynecology and Biology (D.L.F.), University of Michigan, Ann Arbor, Michigan 48109

Address all correspondence and requests for reprints to: Dr. Kei-ichiro Maeda, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. E-mail: keimaeda{at}agr.nagoya-u.ac.jp

Pancreatic glucokinase (GK) is considered an important element of the glucose-sensing unit in pancreatic ß-cells. It is possible that the brain uses similar glucose-sensing units, and we employed GK immunohistochemistry and confocal microscopy to examine the anatomical distribution of GK-like immunoreactivities in the rat brain. We found strong GK-like immunoreactivities in the ependymocytes, endothelial cells, and many serotonergic neurons. In the ependymocytes, the GK-like immunoreactivity was located in the cytoplasmic area, but not in the nucleus. The GK-positive ependymocytes were found to have glucose transporter-2 (GLUT2)-like immunoreactivities on the cilia. In addition, the ependymocytes had GLUT1-like immunoreactivity on the cilia and GLUT4-like immunoreactivity densely in the cytoplasmic area and slightly in the plasma membrane. In serotonergic neurons, GK-like immunoreactivity was found in the cytoplasm and their processes. The present results raise the possibility that these GK-like immunopositive cells comprise a part of a vast glucose-sensing mechanism in the brain.




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