help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alliston, T. N.
Right arrow Articles by Richards, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alliston, T. N.
Right arrow Articles by Richards, J. S.
Endocrinology Vol. 141, No. 1 385-395
Copyright © 2000 by The Endocrine Society

ARTICLES

Expression and Localization of Serum/Glucocorticoid-Induced Kinase in the Rat Ovary: Relation to Follicular Growth and Differentiation1

Tamara N. Alliston, Ignacio J. Gonzalez-Robayna, Patricia Buse, Gary L. Firestone and JoAnne S. Richards

Department of Molecular and Cellular Biology, Baylor College of Medicine (T.N.A., I.J.G.R., J.S.R.), Houston, Texas 77030; and the Department of Molecular and Cell Biology, University of California (P.B., G.L.F.), Berkeley, California 94720

Address all correspondence and requests for reprints to: Dr. JoAnne S. Richards, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030. E-mail: joanner{at}bcm.tmc.edu

Expression of serum/glucocorticoid-inducible kinase (Sgk), one member of an inducible serine/threonine kinase family, is induced by FSH/cAMP in rat granulosa cells cultured in defined medium. The FSH-stimulated pattern of sgk expression is biphasic, and transcriptional activation of the sgk gene depends on an intact Sp1/Sp3 binding site within the proximal promoter. To determine whether sgk was expressed in a hormone-dependent and physiologically relevant manner in vivo, the cellular levels of sgk messenger RNA (mRNA) and protein as well as the subcellular localization of this kinase were analyzed in ovaries containing follicles and corpora lutea at specific stages of differentiation. To stimulate follicular development and luteinization, hypophysectomized (H) rats were treated with estradiol (E; HE) and FSH (FSH; HEF) followed by hCG (hCG; HEF/hCG). To analyze Sgk in functional corpora lutea, PRL was administered to HEF/hCG rats, or ovaries of pregnant rats were obtained on day 7, 15, or 22 of gestation. In situ hybridization indicated that sgk mRNA was low/undetectable in granulosa cells of H and HE rats. An acute injection (iv) of FSH to HE rats rapidly increased sgk mRNA at 2 and 8 h. Sgk mRNA was also elevated in granulosa cells of preovulatory follicles of HEF rats and in luteal cells of HEF/hCG and pregnant rats. Northern blots and Western blots confirmed the in situ hybridization data, indicating that the amount and cellular localization Sgk protein were related to that of sgk mRNA. When the subcellular localization of this kinase was analyzed by immunohistochemistry, Sgk protein was nuclear in granulosa cells and some thecal cells of large preovulatory follicles. In contrast, Sgk protein was cytoplasmic in luteal cells as well as some cells within the stromal compartment. Intense immunostaining was also observed in oocytes present in primordial follicles, but not in growing follicles. Collectively, these results show that FSH and LH stimulate marked increases in the cellular content of Sgk, as well as dramatic changes in the subcellular distribution of this kinase. The specific nuclear vs. cytoplasmic compartmentalization of Sgk in granulosa cells and luteal cells, respectively, indicates that Sgk controls distinct functions in proliferative vs. terminally differentiated granulosa cells.




This article has been cited by other articles:


Home page
 DevelopmentHome page
H.-Y. Fan, M. Shimada, Z. Liu, N. Cahill, N. Noma, Y. Wu, J. Gossen, and J. S. Richards
Selective expression of KrasG12D in granulosa cells of the mouse ovary causes defects in follicle development and ovulation
Development, June 15, 2008; 135(12): 2127 - 2137.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. Stocco, C. Telleria, and G. Gibori
The Molecular Control of Corpus Luteum Formation, Function, and Regression
Endocr. Rev., February 1, 2007; 28(1): 117 - 149.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
F. Lang, C. Bohmer, M. Palmada, G. Seebohm, N. Strutz-Seebohm, and V. Vallon
(Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms.
Physiol Rev, October 1, 2006; 86(4): 1151 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. F. Arteaga, L. Wang, T. Ravid, M. Hochstrasser, and C. M. Canessa
An amphipathic helix targets serum and glucocorticoid-induced kinase 1 to the endoplasmic reticulum-associated ubiquitin-conjugation machinery
PNAS, July 25, 2006; 103(30): 11178 - 11183.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
F. Grahammer, G. Henke, C. Sandu, R. Rexhepaj, A. Hussain, B. Friedrich, T. Risler, M. Metzger, L. Just, T. Skutella, et al.
Intestinal function of gene-targeted mice lacking serum- and glucocorticoid-inducible kinase 1
Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1114 - G1123.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. David, S. L. Stegenga, P. Hu, G. Xiong, E. Kerr, K. B. Becker, S. Venkatapathy, J. A. Warrington, and R. G. Kalb
Expression of Serum- and Glucocorticoid-Inducible Kinase Is Regulated in an Experience-Dependent Manner and Can Cause Dendrite Growth
J. Neurosci., July 27, 2005; 25(30): 7048 - 7053.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Jo, M. C. Gieske, C. E. Payne, S. E. Wheeler-Price, J. B. Gieske, I. V. Ignatius, T. E. Curry Jr., and C. Ko
Development and Application of a Rat Ovarian Gene Expression Database
Endocrinology, November 1, 2004; 145(11): 5384 - 5396.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Palmada, M. Dieter, A. Speil, C. Bohmer, A. F. Mack, H. J. Wagner, K. Klingel, R. Kandolf, H. Murer, J. Biber, et al.
Regulation of intestinal phosphate cotransporter NaPi IIb by ubiquitin ligase Nedd4-2 and by serum- and glucocorticoid-dependent kinase 1
Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G143 - G150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Coric, N. Hernandez, D. A. de la Rosa, D. Shao, T. Wang, and C. M. Canessa
Expression of ENaC and serum- and glucocorticoid-induced kinase 1 in the rat intestinal epithelium
Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G663 - G670.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Yang and S. K. Roy
Follicle Stimulating Hormone-Induced DNA Synthesis in the Granulosa Cells of Hamster Preantral Follicles Involves Activation of Cyclin-Dependent Kinase-4 Rather Than Cyclin D2 Synthesis
Biol Reprod, February 1, 2004; 70(2): 509 - 517.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Hsieh, S. M. Mulders, R. R. Friis, A. Dharmarajan, and J. S. Richards
Expression and Localization of Secreted Frizzled-Related Protein-4 in the Rodent Ovary: Evidence for Selective Up-Regulation in Luteinized Granulosa Cells
Endocrinology, October 1, 2003; 144(10): 4597 - 4606.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. L. Russell, K. M. H. Doyle, I. Gonzales-Robayna, C. Pipaon, and J. S. Richards
Egr-1 Induction in Rat Granulosa Cells by Follicle-Stimulating Hormone and Luteinizing Hormone: Combinatorial Regulation By Transcription Factors Cyclic Adenosine 3',5'-Monophosphate Regulatory Element Binding Protein, Serum Response Factor, Sp1, and Early Growth Response Factor-1
Mol. Endocrinol., April 1, 2003; 17(4): 520 - 533.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
C. Boehmer, V. Wilhelm, M. Palmada, S. Wallisch, G. Henke, H. Brinkmeier, P. Cohen, B. Pieske, and F. Lang
Serum and glucocorticoid inducible kinases in the regulation of the cardiac sodium channel SCN5A
Cardiovasc Res, March 15, 2003; 57(4): 1079 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. C. Maiyar, M. L.L. Leong, and G. L. Firestone
Importin-alpha Mediates the Regulated Nuclear Targeting of Serum- and Glucocorticoid-inducible Protein Kinase (Sgk) by Recognition of a Nuclear Localization Signal in the Kinase Central Domain
Mol. Biol. Cell, March 1, 2003; 14(3): 1221 - 1239.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S. Chu, S. Rushdi, E.T. Zumpe, P. Mamers, D.L. Healy, T. Jobling, H.G. Burger, and P.J. Fuller
FSH-regulated gene expression profiles in ovarian tumours and normal ovaries
Mol. Hum. Reprod., May 1, 2002; 8(5): 426 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Stockand
New ideas about aldosterone signaling in epithelia
Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. S. Richards, S. C. Sharma, A. E. Falender, and Y. H. Lo
Expression of FKHR, FKHRL1, and AFX Genes in the Rodent Ovary: Evidence for Regulation by IGF-I, Estrogen, and the Gonadotropins
Mol. Endocrinol., March 1, 2002; 16(3): 580 - 599.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Hsieh, M. A. Johnson, N. M. Greenberg, and J. S. Richards
Regulated Expression of Wnts and Frizzleds at Specific Stages of Follicular Development in the Rodent Ovary
Endocrinology, March 1, 2002; 143(3): 898 - 908.
[Abstract] [Full Text] [PDF]

Home page
 Recent Prog Horm ResHome page
J. S. Richards, D. L. Russell, S. Ochsner, M. Hsieh, K. H. Doyle, A. E. Falender, Y. K. Lo, and S. C. Sharma
Novel Signaling Pathways That Control Ovarian Follicular Development, Ovulation, and Luteinization
Recent Prog. Horm. Res., January 1, 2002; 57(1): 195 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
F. Lang and P. Cohen
Regulation and Physiological Roles of Serum- and Glucocorticoid-Induced Protein Kinase Isoforms
Sci. Signal., November 13, 2001; 2001(108): re17 - re17.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. H. Burns, C. Yan, T. R. Kumar, and M. M. Matzuk
Analysis of Ovarian Gene Expression in Follicle-Stimulating Hormone {beta} Knockout Mice
Endocrinology, July 1, 2001; 142(7): 2742 - 2751.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. S. Richards
Perspective: The Ovarian Follicle--A Perspective in 2001
Endocrinology, June 1, 2001; 142(6): 2184 - 2193.
[Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. S. Richards
New Signaling Pathways for Hormones and Cyclic Adenosine 3',5'-Monophosphate Action in Endocrine Cells
Mol. Endocrinol., February 1, 2001; 15(2): 209 - 218.
[Abstract] [Full Text]

Home page
 Reproductive SciencesHome page
J. S. Richards
Graafian Follicle Function and Luteinization in Nonprimates
Reproductive Sciences, January 1, 2001; 8(1_suppl): S21 - S23.
[Abstract] [PDF]

Home page
 Mol. Endocrinol.Home page
I. J. Gonzalez-Robayna, A. E. Falender, S. Ochsner, G. L. Firestone, and J. S. Richards
Follicle-Stimulating Hormone (FSH) Stimulates Phosphorylation and Activation of Protein Kinase B (PKB/Akt) and Serum and Glucocorticoid-Induced Kinase (Sgk): Evidence for A Kinase-Independent Signaling by FSH in Granulosa Cells
Mol. Endocrinol., August 1, 2000; 14(8): 1283 - 1300.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. C. Sharma and J. S. Richards
Regulation of AP1 (Jun/Fos) Factor Expression and Activation in Ovarian Granulosa Cells. RELATION OF JunD AND Fra2 TO TERMINAL DIFFERENTIATION
J. Biol. Chem., October 20, 2000; 275(43): 33718 - 33728.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society