help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshioka, S.
Right arrow Articles by Espey, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshioka, S.
Right arrow Articles by Espey, L. L.
Endocrinology Vol. 141, No. 11 4114-4119
Copyright © 2000 by The Endocrine Society

ARTICLES

Expression of Tumor Necrosis Factor-Stimulated Gene-6 in the Rat Ovary in Response to an Ovulatory Dose of Gonadotropin1

Shinya Yoshioka, Scott Ochsner, Darryl L. Russell, Takeshi Ujioka, Shingo Fujii, Joanne S. Richards and Lawrence L. Espey

Department of Biology (S.Y., T.U., L.L.E.), Trinity University, San Antonio, Texas 78212; Department of Cell Biology (S.O., D.L.R., J.S.R.), Baylor College of Medicine, Houston, Texas 77030; and Department of Gynecology and Obstetrics (S.F.), Kyoto University School of Medicine, Kyoto 606, Japan

Address all correspondence and requests for reprints to: Lawrence Espey, Ph.D., Department of Biology, Trinity University, San Antonio, Texas 78212. E-mail: lespey{at}trinity.edu

Current evidence supports the hypothesis that the biochemical events of mammalian ovulation are analogous to an acute inflammatory reaction. This study reveals that tumor necrosis factor-stimulated gene-6 (TSG-6), which encodes a member of the superfamily of hyaluronan-binding proteins that is specifically translated in inflammatory reactions, is expressed in ovarian follicles that have been induced to ovulate. Immature Wistar rats were primed with 10 IU equine CG sc; and 48 h later, the 12-h ovulatory process was initiated by 10 IU human CG (hCG), sc. Ovarian RNA was extracted at 0, 2, 4, 8, 12, and 24 h after the primed animals were injected with hCG. The RNA extracts were used for RT-PCR differential display of amplified complementary DNAs (cDNAs) that represented gene expression in the stimulated ovarian tissue. Northern analysis of one of the differentially amplified cDNAs confirmed that it was part of a gene that was substantially up-regulated at 4–8 h after the ovaries had been stimulated by hCG. Subcloning and sequence analysis revealed that the cDNA matched the gene for TSG-6. In situ hybridization indicated that the TSG-6 messenger RNA was primarily located in the cumulus mass and the antral granulosa cells of large ovarian follicles. In conclusion, the data show that expression of TSG-6 is an integral part of the cascade of inflammatory-like changes that occur in an ovulatory follicle in response to a trophic hormone that couples with luteinizing hormone/hCG receptors.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
E. Nagyova, A. Camaioni, R. Prochazka, A. J. Day, and A. Salustri
Synthesis of Tumor Necrosis Factor Alpha-Induced Protein 6 in Porcine Preovulatory Follicles: A Study with A38 Antibody
Biol Reprod, May 1, 2008; 78(5): 903 - 909.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Scarchilli, A. Camaioni, B. Bottazzi, V. Negri, A. Doni, L. Deban, A. Bastone, G. Salvatori, A. Mantovani, G. Siracusa, et al.
PTX3 Interacts with Inter-{alpha}-trypsin Inhibitor: IMPLICATIONS FOR HYALURONAN ORGANIZATION AND CUMULUS OOPHORUS EXPANSION
J. Biol. Chem., October 12, 2007; 282(41): 30161 - 30170.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
D. L. Russell and R. L. Robker
Molecular mechanisms of ovulation: co-ordination through the cumulus complex
Hum. Reprod. Update, May 1, 2007; 13(3): 289 - 312.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. Sayasith, M. Dore, and J. Sirois
Molecular characterization of tumor necrosis {alpha}-induced protein 6 and its human chorionic gonadotropin-dependent induction in theca and mural granulosa cells of equine preovulatory follicles
Reproduction, January 1, 2007; 133(1): 135 - 145.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Takahashi, J. D. Morrow, H. Wang, and S. K. Dey
Cyclooxygenase-2-derived Prostaglandin E2 Directs Oocyte Maturation by Differentially Influencing Multiple Signaling Pathways
J. Biol. Chem., December 1, 2006; 281(48): 37117 - 37129.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
A Hourvitz, E Gershon, J D Hennebold, S Elizur, E Maman, C Brendle, E Y Adashi, and N Dekel
Ovulation-selective genes: the generation and characterization of an ovulatory-selective cDNA library.
J. Endocrinol., March 1, 2006; 188(3): 531 - 548.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. Pakarainen, F.-P. Zhang, L. Nurmi, M. Poutanen, and I. Huhtaniemi
Knockout of Luteinizing Hormone Receptor Abolishes the Effects of Follicle-Stimulating Hormone on Preovulatory Maturation and Ovulation of Mouse Graafian Follicles
Mol. Endocrinol., October 1, 2005; 19(10): 2591 - 2602.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. M. A. Tullet, V. Pocock, J. H. Steel, R. White, S. Milligan, and M. G. Parker
Multiple Signaling Defects in the Absence of RIP140 Impair Both Cumulus Expansion and Follicle Rupture
Endocrinology, September 1, 2005; 146(9): 4127 - 4137.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. Rugg, A. C. Willis, D. Mukhopadhyay, V. C. Hascall, E. Fries, C. Fulop, C. M. Milner, and A. J. Day
Characterization of Complexes Formed between TSG-6 and Inter-{alpha}-inhibitor That Act as Intermediates in the Covalent Transfer of Heavy Chains onto Hyaluronan
J. Biol. Chem., July 8, 2005; 280(27): 25674 - 25686.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
E. Nagyova, A. Camaioni, R. Prochazka, and A. Salustri
Covalent Transfer of Heavy Chains of Inter-{alpha}-Trypsin Inhibitor Family Proteins to Hyaluronan in In Vivo and In Vitro Expanded Porcine Oocyte-Cumulus Complexes
Biol Reprod, December 1, 2004; 71(6): 1838 - 1843.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Jo, M. C. Gieske, C. E. Payne, S. E. Wheeler-Price, J. B. Gieske, I. V. Ignatius, T. E. Curry Jr., and C. Ko
Development and Application of a Rat Ovarian Gene Expression Database
Endocrinology, November 1, 2004; 145(11): 5384 - 5396.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Shimada, M. Nishibori, Y. Yamashita, J. Ito, T. Mori, and J. S. Richards
Down-Regulated Expression of A Disintegrin and Metalloproteinase with Thrombospondin-Like Repeats-1 by Progesterone Receptor Antagonist Is Associated with Impaired Expansion of Porcine Cumulus-Oocyte Complexes
Endocrinology, October 1, 2004; 145(10): 4603 - 4614.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Salustri, C. Garlanda, E. Hirsch, M. De Acetis, A. Maccagno, B. Bottazzi, A. Doni, A. Bastone, G. Mantovani, P. B. Peccoz, et al.
PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization
Development, April 1, 2004; 131(7): 1577 - 1586.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Mukhopadhyay, A. Asari, M. S. Rugg, A. J. Day, and C. Fulop
Specificity of the Tumor Necrosis Factor-induced Protein 6-mediated Heavy Chain Transfer from Inter-{alpha}-trypsin Inhibitor to Hyaluronan: IMPLICATIONS FOR THE ASSEMBLY OF THE CUMULUS EXTRACELLULAR MATRIX
J. Biol. Chem., March 19, 2004; 279(12): 11119 - 11128.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T. Fayad, V. Levesque, J. Sirois, D. W. Silversides, and J. G. Lussier
Gene Expression Profiling of Differentially Expressed Genes in Granulosa Cells of Bovine Dominant Follicles Using Suppression Subtractive Hybridization
Biol Reprod, February 1, 2004; 70(2): 523 - 533.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. L. Russell, K. M. H. Doyle, S. A. Ochsner, J. D. Sandy, and J. S. Richards
Processing and Localization of ADAMTS-1 and Proteolytic Cleavage of Versican during Cumulus Matrix Expansion and Ovulation
J. Biol. Chem., October 24, 2003; 278(43): 42330 - 42339.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. A. Ochsner, A. J. Day, M. S. Rugg, R. M. Breyer, R. H. Gomer, and J. S. Richards
Disrupted Function of Tumor Necrosis Factor-{alpha}-Stimulated Gene 6 Blocks Cumulus Cell-Oocyte Complex Expansion
Endocrinology, October 1, 2003; 144(10): 4376 - 4384.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. Milner and A. J. Day
TSG-6: a multifunctional protein associated with inflammation
J. Cell Sci., May 15, 2003; 116(10): 1863 - 1873.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Fulop, S. Szanto, D. Mukhopadhyay, T. Bardos, R. V. Kamath, M. S. Rugg, A. J. Day, A. Salustri, V. C. Hascall, T. T. Glant, et al.
Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice
Development, May 15, 2003; 130(10): 2253 - 2261.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. W. Sun, H. Kobayashi, M. Suzuki, N. Kanayama, and T. Terao
Follicle-Stimulating Hormone and Insulin-Like Growth Factor I Synergistically Induce Up-Regulation of Cartilage Link Protein (Crtl1) via Activation of Phosphatidylinositol-Dependent Kinase/Akt in Rat Granulosa Cells
Endocrinology, March 1, 2003; 144(3): 793 - 801.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. A. Ochsner, D. L. Russell, A. J. Day, R. M. Breyer, and J. S. Richards
Decreased Expression of Tumor Necrosis Factor-{alpha}-Stimulated Gene 6 in Cumulus Cells of the Cyclooxygenase-2 and EP2 Null Mice
Endocrinology, March 1, 2003; 144(3): 1008 - 1019.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. L. Russell, S. A. Ochsner, M. Hsieh, S. Mulders, and J. S. Richards
Hormone-Regulated Expression and Localization of Versican in the Rodent Ovary
Endocrinology, March 1, 2003; 144(3): 1020 - 1031.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. L. Espey and J. S. Richards
Temporal and Spatial Patterns of Ovarian Gene Transcription Following an Ovulatory Dose of Gonadotropin in the Rat
Biol Reprod, December 1, 2002; 67(6): 1662 - 1670.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Varani, J. A. Elvin, C. Yan, J. DeMayo, F. J. DeMayo, H. F. Horton, M. C. Byrne, and M. M. Matzuk
Knockout of Pentraxin 3, a Downstream Target of Growth Differentiation Factor-9, Causes Female Subfertility
Mol. Endocrinol., June 1, 2002; 16(6): 1154 - 1167.
[Abstract] [Full Text] [PDF]

Home page
 Recent Prog Horm ResHome page
J. S. Richards, D. L. Russell, S. Ochsner, M. Hsieh, K. H. Doyle, A. E. Falender, Y. K. Lo, and S. C. Sharma
Novel Signaling Pathways That Control Ovarian Follicular Development, Ovulation, and Luteinization
Recent Prog. Horm. Res., January 1, 2002; 57(1): 195 - 220.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. D'Alessandris, R. Canipari, M. Di Giacomo, O. Epifano, A. Camaioni, G. Siracusa, and A. Salustri
Control of Mouse Cumulus Cell-Oocyte Complex Integrity before and after Ovulation: Plasminogen Activator Synthesis and Matrix Degradation
Endocrinology, July 1, 2001; 142(7): 3033 - 3040.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. S. Richards
Perspective: The Ovarian Follicle--A Perspective in 2001
Endocrinology, June 1, 2001; 142(6): 2184 - 2193.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society