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Endocrinology Vol. 141, No. 11 4114-4119
Copyright © 2000 by The Endocrine Society

ARTICLES

Expression of Tumor Necrosis Factor-Stimulated Gene-6 in the Rat Ovary in Response to an Ovulatory Dose of Gonadotropin1

Shinya Yoshioka, Scott Ochsner, Darryl L. Russell, Takeshi Ujioka, Shingo Fujii, Joanne S. Richards and Lawrence L. Espey

Department of Biology (S.Y., T.U., L.L.E.), Trinity University, San Antonio, Texas 78212; Department of Cell Biology (S.O., D.L.R., J.S.R.), Baylor College of Medicine, Houston, Texas 77030; and Department of Gynecology and Obstetrics (S.F.), Kyoto University School of Medicine, Kyoto 606, Japan

Address all correspondence and requests for reprints to: Lawrence Espey, Ph.D., Department of Biology, Trinity University, San Antonio, Texas 78212. E-mail: lespey{at}trinity.edu

Current evidence supports the hypothesis that the biochemical events of mammalian ovulation are analogous to an acute inflammatory reaction. This study reveals that tumor necrosis factor-stimulated gene-6 (TSG-6), which encodes a member of the superfamily of hyaluronan-binding proteins that is specifically translated in inflammatory reactions, is expressed in ovarian follicles that have been induced to ovulate. Immature Wistar rats were primed with 10 IU equine CG sc; and 48 h later, the 12-h ovulatory process was initiated by 10 IU human CG (hCG), sc. Ovarian RNA was extracted at 0, 2, 4, 8, 12, and 24 h after the primed animals were injected with hCG. The RNA extracts were used for RT-PCR differential display of amplified complementary DNAs (cDNAs) that represented gene expression in the stimulated ovarian tissue. Northern analysis of one of the differentially amplified cDNAs confirmed that it was part of a gene that was substantially up-regulated at 4–8 h after the ovaries had been stimulated by hCG. Subcloning and sequence analysis revealed that the cDNA matched the gene for TSG-6. In situ hybridization indicated that the TSG-6 messenger RNA was primarily located in the cumulus mass and the antral granulosa cells of large ovarian follicles. In conclusion, the data show that expression of TSG-6 is an integral part of the cascade of inflammatory-like changes that occur in an ovulatory follicle in response to a trophic hormone that couples with luteinizing hormone/hCG receptors.




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