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Endocrinology Vol. 141, No. 2 615-620
Copyright © 2000 by The Endocrine Society

ARTICLES

Ontogenetic Changes in the Expression of Estrogen Receptor {alpha} and ß in Rat Pituitary Gland Detected by Immunohistochemistry

Eijun Nishihara, Yuji Nagayama, Satoshi Inoue, Hisahiko Hiroi, Masami Muramatsu, Shunichi Yamashita and Takehiko Koji

Department of Nature Medicine, Atomic Bomb Disease Institute (E.N., S.Y.), and the Departments of Pharmacology I (Y.N.) and Histology and Cell Biology (T.K.), Nagasaki University School of Medicine, Nagasaki 852-8523; and the Department of Biochemistry, Saitama Medical School (S.I., H.H., M.M.), Saitama 350-0495, Japan

Address all correspondence and requests for reprints to: Eijun Nishihara, M.D., Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Sakamoto 1–12-4, Nagasaki 852-8523, Japan. E-mail: eijun-ngs{at}umin.ac.jp

The physiological effects of estrogen on the pituitary, including cellular proliferation and regulation of hormone synthesis, are mediated by the nuclear estrogen receptor (ER). The purpose of this study was to determine ontogenetic expression of two types of ERs (ER{alpha} and ERß) in the pituitary using specific antibodies, monoclonal antibody (1D5) for ER{alpha} and polyclonal antibody generated against ERß. First, we confirmed the detection of 66- and 55-kDa bands for ER{alpha} and ERß, respectively, in the rat pituitary extract by Western blotting. Then immunostaining with these antibodies was performed using fetal and adult Wistar rat tissues, combined with PRL or LHß immunohistochemistry. Intense ERß signal was detected throughout the pituitary from day 12 of gestation. However, staining for ER{alpha} only became detectable from day 17 of gestation. In contrast with the fetal period, nuclei stained for ER{alpha} were widely distributed in the anterior lobe in the adult rat, whereas ERß-positive cells were restricted in the anterior lobe. LHß, but not PRL, was colocalized in ERß-positive cells. Our results indicated that the major population of ER subtypes in the rat pituitary gland has changed around the day of birth and that the expression of ERß may be involved in the differentiation of pituitary cell function to synthesize a specific hormone.




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