This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tollet-Egnell, P. Articles by Norstedt, G. Search for Related Content PubMed PubMed Citation Articles by Tollet-Egnell, P. Articles by Norstedt, G.

Differential Cloning of Growth Hormone-Regulated Hepatic Transcripts in the Aged Rat¹

Department of Molecular Medicine, Karolinska Institute, Karolinska Hospital (P.T.-E., A.F.-M., G.N.), 171 76 Stockholm; and the Department of Biochemistry and Biotechnology, Royal Institute of Technology (J.O., J.L.), 100 44 Stockholm, Sweden

Address all correspondence and requests for reprints to: Dr. Petra Tollet-Egnell, Department of Molecular Medicine, Karolinska Institute, CMM L8:01, Karolinska Hospital, 171 76 Stockholm, Sweden. E-mail: petra.tollet.egnell{at}molmed.ki.se

It has been suggested that aging or at least some of its symptoms are related to a physiological decline in GH levels with age. This study was performed to elucidate age-related changes in GH-dependent effects at the level of gene expression. Through the application of complementary DNA representational difference analysis (RDA) we have identified gene products that are reduced during aging in rat liver. The expression of these genes was restored upon GH treatment. Results from reverse Northern and ribonuclease protection analysis confirmed that the RDA products were truly differentially expressed. In addition to well characterized GH-regulated genes, including CYP2C12, CYP2C13, and _2u-globulin, we demonstrate the differential expression of at least 11 genes previously not known to be under GH control. Several hepatic transcripts encoding enzymes and receptors involved in the metabolism of protein, carbohydrates, and lipids were identified. Other RDA products consisted of transcripts encoding proteins involved in ATP synthesis, detoxification of reactive oxygen species, or immune responses. This list of GH-regulated genes in the old rat may shed further light on the action and mechanism behind the positive effects of GH on, for example, body composition and the immune system that have been observed in different animal and human studies.

This article has been cited by other articles:

				M. Garcia-Fernandez, G. Delgado, J. E. Puche, S. Gonzalez-Baron, and I. Castilla Cortazar Low Doses of Insulin-Like Growth Factor I Improve Insulin Resistance, Lipid Metabolism, and Oxidative Damage in Aging Rats Endocrinology, May 1, 2008; 149(5): 2433 - 2442. [Abstract] [Full Text] [PDF]

				M. Iruthayanathan, Y.-H. Zhou, and G. V. Childs Dehydroepiandrosterone Restoration of Growth Hormone Gene Expression in Aging Female Rats, in Vivo and in Vitro: Evidence for Actions via Estrogen Receptors Endocrinology, December 1, 2005; 146(12): 5176 - 5187. [Abstract] [Full Text] [PDF]

				C. Ameen, D. Linden, B.-M. Larsson, A. Mode, A. Holmang, and J. Oscarsson Effects of gender and GH secretory pattern on sterol regulatory element-binding protein-1c and its target genes in rat liver Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1039 - E1048. [Abstract] [Full Text] [PDF]

				P. Tollet-Egnell, P. Parini, N. Stahlberg, I. Lonnstedt, N. H. Lee, M. Rudling, A. Flores-Morales, and G. Norstedt Growth hormone-mediated alteration of fuel metabolism in the aged rat as determined from transcript profiles Physiol Genomics, January 15, 2004; 16(2): 261 - 267. [Abstract] [Full Text] [PDF]

				K. Dillner, J. Kindblom, A. Flores-Morales, R. Shao, J. Tornell, G. Norstedt, and H. Wennbo Gene Expression Analysis of Prostate Hyperplasia in Mice Overexpressing the Prolactin Gene Specifically in the Prostate Endocrinology, November 1, 2003; 144(11): 4955 - 4966. [Abstract] [Full Text] [PDF]

				X. Wu, S.-T. Pang, L. Sahlin, A. Blanck, G. Norstedt, and A. Flores-Morales Gene Expression Profiling of the Effects of Castration and Estrogen Treatment in the Rat Uterus Biol Reprod, October 1, 2003; 69(4): 1308 - 1317. [Abstract] [Full Text] [PDF]

				B. Olsson, M. Bohlooly-Y, O. Brusehed, O. G. P. Isaksson, B. Ahren, S.-O. Olofsson, J. Oscarsson, and J. Tornell Bovine growth hormone-transgenic mice have major alterations in hepatic expression of metabolic genes Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E504 - E511. [Abstract] [Full Text] [PDF]

				X. Wu, A. Blanck, G. Norstedt, L. Sahlin, and A. Flores-Morales Identification of genes with higher expression in human uterine leiomyomas than in the corresponding myometrium Mol. Hum. Reprod., March 1, 2002; 8(3): 246 - 254. [Abstract] [Full Text] [PDF]

				A. Flores-Morales, N. Stahlberg, P. Tollet-Egnell, J. Lundeberg, R. L. Malek, J. Quackenbush, N. H. Lee, and G. Norstedt Microarray Analysis of the in Vivo Effects of Hypophysectomy and Growth Hormone Treatment on Gene Expression in the Rat Endocrinology, July 1, 2001; 142(7): 3163 - 3176. [Abstract] [Full Text] [PDF]

				P. Tollet-Egnell, A. Flores-Morales, N. Ståhlberg, R. L. Malek, N. Lee, and G. Norstedt Gene Expression Profile of the Aging Process in Rat Liver: Normalizing Effects of Growth Hormone Replacement Mol. Endocrinol., February 1, 2001; 15(2): 308 - 318. [Abstract] [Full Text]