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Endocrinology Vol. 141, No. 4 1521-1527
Copyright © 2000 by The Endocrine Society

ARTICLES

Interleukin-1 Receptor Antagonist Is Produced by Sertoli Cells in Vitro1

Daniel Zeyse2, Eitan Lunenfeld, Melanie Beck, Isebrand Prinsloo and Mahmoud Huleihel

Department of Obstetrics and Gynecology, Soroka University Medical Center (E.L., M.H.) and Department of Microbiology and Immunology (M.H.) and Department of Pathology (I.P.), Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel; Christian-Albrechts-University of Kiel Medical School (D.Z., M.B.), Kiel, Germany

Address all correspondence and requests for reprints to: Mahmoud Huleihel, Ph.D., Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. E-mail: huleihel{at}bgumail.bgu.ac.il

The interleukin-1 (IL-1) system has been suggested to be involved in the cell-to-cell cross-talk within the testis. To identify a testicular cell source of IL-1 receptor antagonist (IL-1ra), mouse Sertoli cells were isolated, purified, cultured, and examined for IL-1ra. Our investigation revealed that Sertoli cells produce large amounts of immunoreactive IL-1ra under basal culture conditions, as examined by enzyme-linked immunosorbent assays. Its expression can be induced, showing maximum concentrations after 8 h of stimulation. Lipopolysaccharide, as well as IL-1{alpha} and -ß, were found to stimulate IL-1ra production in Sertoli cells. FSH is capable to induce IL-1ra production in Sertoli cells in a dose-dependent manner. Immunocytochemical staining confirmed the presence of IL-1ra in the cytoplasma of Sertoli cells. The presence of IL-1ra messenger RNA was demonstrated by RT-PCR analysis. Our results may help to better evaluate the IL-1 activity in the testis and may indicate the involvement of IL-1ra in the autocrine and paracrine regulation of testicular cell function.




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