help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boue-Grabot, E.
Right arrow Articles by Garret, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boue-Grabot, E.
Right arrow Articles by Garret, M.
Endocrinology Vol. 141, No. 5 1627-1632
Copyright © 2000 by The Endocrine Society

ARTICLES

Molecular and Electrophysiological Evidence for a GABAC Receptor in Thyrotropin-Secreting Cells1

Eric Boue-Grabot2, Anne Taupignon, Gérard Tramu and Maurice Garret

Laboratoire de Neurophysiologie Centre National de la Recherche Scientifique-Unité Mixte de Recherche 5543 (E.B.-G., A.T., M.G.), Université Victor Segalen Bordeaux 2, 33076 Bordeaux Cedex, France and Laboratoire de Neurocytochimie Fonctionnelle (G.T.), Centre National de la Recherche Scientifique-Unité Mixte de Recherche 5807, Université de Bordeaux 1, 33405 Talence Cedex, France

Address all correspondence and requests for reprints to: M. Garret, Laboratoire de Neurophysiologie, Unité Mixte de Recherche 5543, Université de Bordeaux 2, 146 rue Léo-Saignat 33076 Bordeaux cedex, France. E-mail: maurice.garret{at}umr5543.u-bordeaux2.fr

In the pituitary, GABA regulates the release of several hormones via different receptors. GABAC receptors are heterooligomers that differ from GABAA receptors in that they contain {rho}-subunits and are insensitive to bicuculline. However, molecular and functional evidence for the presence of GABAC receptors outside the retina has yet to be established. The present work was performed on guinea pig and rat pituitaries. Both Northern blot and RT-PCR analysis showed that, although {rho}1- and {rho}2-subunits were expressed at similar levels in the rat retina, {rho}1 messenger RNA (mRNA) was enriched, relative to {rho}2 mRNA in the rat pituitary. Northern blot experiments also showed that, in the pituitary, {rho}1 and {rho}2 mRNAs are shorter in size than those expressed in the retina. The use of a subunit-specific antibody revealed colocalization of {rho}1-subunit and anti-TSH labeling on rat pituitary sections. TSH guinea pig pituitary cells were also labeled with a {rho}-subunit antiserum. Moreover, whole-cell patch clamp on single guinea pig TSH cells showed that GABA induced a bicuculline- insensitive Cl- current. In contrast to the Cl- current generated by GABAC receptors in the retina, the bicuculline-insensitive Cl- currents in TSH cells quickly desensitized. These results suggest that a novel GABAC receptor may regulate TSH secretion and that the structure and/or biochemical regulation of this pituitary receptor is different from that found in the retina.




This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
M. Chebib, T. Hinton, K. L. Schmid, D. Brinkworth, H. Qian, S. Matos, H.-L. Kim, H. Abdel-Halim, R. J. Kumar, G. A. R. Johnston, et al.
Novel, Potent, and Selective GABAC Antagonists Inhibit Myopia Development and Facilitate Learning and Memory
J. Pharmacol. Exp. Ther., February 1, 2009; 328(2): 448 - 457.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
H. W. Zemkova, I. Bjelobaba, M. Tomic, H. Zemkova, and S. S. Stojilkovic
Molecular, pharmacological and functional properties of GABAA receptors in anterior pituitary cells
J. Physiol., July 1, 2008; 586(13): 3097 - 3111.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Boue-Grabot, M. B. Emerit, E. Toulme, P. Seguela, and M. Garret
Cross-talk and Co-trafficking between {rho}1/GABA Receptors and ATP-gated Channels
J. Biol. Chem., February 20, 2004; 279(8): 6967 - 6975.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Zheng, W. Xie, J. Zhang, J. A. Strong, L. Wang, L. Yu, M. Xu, and L. Lu
Function of {gamma}-Aminobutyric Acid Receptor/Channel {rho}1 Subunits In Spinal Cord
J. Biol. Chem., November 28, 2003; 278(48): 48321 - 48329.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Gamel-Didelon, L. Kunz, K. J. Fohr, M. Gratzl, and A. Mayerhofer
Molecular and Physiological Evidence for Functional {gamma}-Aminobutyric Acid (GABA)-C Receptors in Growth Hormone-secreting Cells
J. Biol. Chem., May 23, 2003; 278(22): 20192 - 20195.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Kehoe and C. Vulfius
Independence of and Interactions between GABA-, Glutamate-, and Acetylcholine-Activated Cl Conductances in Aplysia Neurons
J. Neurosci., December 1, 2000; 20(23): 8585 - 8596.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society