help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duffy, D. M.
Right arrow Articles by Stouffer, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duffy, D. M.
Right arrow Articles by Stouffer, R. L.
Endocrinology Vol. 141, No. 5 1711-1717
Copyright © 2000 by The Endocrine Society

ARTICLES

Expression of Estrogen Receptor {alpha} and ß in the Rhesus Monkey Corpus Luteum during the Menstrual Cycle: Regulation by Luteinizing Hormone and Progesterone1

Diane M. Duffy, Charles L. Chaffin and Richard L. Stouffer

Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton, Oregon 97006

Address all correspondence to: Diane M. Duffy, Division of Reproductive Sciences, Oregon Regional Primate Research Center, 505 NW 185th Avenue, Beaverton, Oregon 97006.

There are conflicting reports on the presence or absence of estrogen receptor (ER) in the primate corpus luteum, and the discovery of a second type of estrogen receptor, ERß, adds an additional level of complexity. To reevaluate ER expression in the primate luteal tissue, we used semiquantitative RT-PCR based assays and Western blotting to assess ER{alpha} and ß messenger RNA (mRNA) and protein levels in corpora lutea (n = 3/stage) obtained from adult female rhesus monkeys at early (days 3–5), mid (days 6–8), mid-late (days 10–12), and late (days 14–16) luteal phase of the natural menstrual cycle. ER{alpha} mRNA levels did not vary across the stages of the luteal phase, and ER{alpha} protein was not consistently detected in luteal tissues. However, ERß mRNA and protein levels were detectable in early and mid luteal phases and increased (P < 0.05) to peak levels at mid-late luteal phase before declining by late luteal phase. To determine if ERß mRNA expression in the corpus luteum is regulated by LH, monkeys received the GnRH antagonist antide either alone or with 3 daily injections of LH to simulate pulsatile LH release. Treatment with antide alone or concomitant LH administration did not alter luteal ERß mRNA levels. When monkeys also received the 3ß-hydroxysteroid dehydrogenase inhibitor trilostane to reduce luteal progesterone production, luteal ERß mRNA levels were 3-fold higher (P < 0.05) than in monkeys receiving antide + LH only. Replacement of progestin activity with R5020 reduced luteal ERß mRNA levels to those seen in animals receiving antide + LH. Thus, there is dynamic ERß expression in the primate corpus luteum during the menstrual cycle, consistent with a role for estrogen in the regulation of primate luteal function and life span via a receptor (ERß)-mediated pathway. Increased ERß expression in the progestin-depleted corpus luteum during LH exposure suggests that the relative progestin deprivation experienced by the corpus luteum between LH pulses may enhance luteal sensitivity to estrogens during the late luteal phase of the menstrual cycle.




This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
R. L. Bogan, M. J. Murphy, R. L. Stouffer, and J. D. Hennebold
Systematic Determination of Differential Gene Expression in the Primate Corpus Luteum during the Luteal Phase of the Menstrual Cycle
Mol. Endocrinol., May 1, 2008; 22(5): 1260 - 1273.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
S. van den Driesche, V. M Smith, M. Myers, and W C. Duncan
Expression and regulation of oestrogen receptors in the human corpus luteum
Reproduction, April 1, 2008; 135(4): 509 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
G. Irusta, M.J. Murphy, W.D. Perez, and J.D. Hennebold
Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing enzymes in the primate corpus luteum
Mol. Hum. Reprod., August 1, 2007; 13(8): 541 - 548.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Miyakoshi, M. J. Murphy, R. R. Yeoman, S. Mitra, C. J. Dubay, and J. D. Hennebold
The Identification of Novel Ovarian Proteases Through the Use of Genomic and Bioinformatic Methodologies
Biol Reprod, December 1, 2006; 75(6): 823 - 835.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. Xu, J. D. Hennebold, and R. L. Stouffer
Dynamic Expression and Regulation of the Corticotropin-Releasing Hormone/Urocortin-Receptor-Binding Protein System in the Primate Ovary during the Menstrual Cycle
J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1544 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. C. Peluffo, K. A. Young, and R. L. Stouffer
Dynamic Expression of Caspase-2, -3, -8, and -9 Proteins and Enzyme Activity, But Not Messenger Ribonucleic Acid, in the Monkey Corpus Luteum during the Menstrual Cycle
J. Clin. Endocrinol. Metab., April 1, 2005; 90(4): 2327 - 2335.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. Xu, R.L. Stouffer, R.P. Searles, and J.D. Hennebold
Discovery of LH-regulated genes in the primate corpus luteum
Mol. Hum. Reprod., March 1, 2005; 11(3): 151 - 159.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. A. Young, B. Tumlinson, and R. L. Stouffer
ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum: divergent patterns and stage-dependent regulation during the natural menstrual cycle
Mol. Hum. Reprod., August 1, 2004; 10(8): 559 - 565.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. J. Diaz and M. C. Wiltbank
Acquisition of Luteolytic Capacity: Changes in Prostaglandin F2{alpha} Regulation of Steroid Hormone Receptors and Estradiol Biosynthesis in Pig Corpora Lutea
Biol Reprod, May 1, 2004; 70(5): 1333 - 1339.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. A. Young and R. L. Stouffer
Gonadotropin and Steroid Regulation of Matrix Metalloproteinases and Their Endogenous Tissue Inhibitors in the Developed Corpus Luteum of the Rhesus Monkey During the Menstrual Cycle
Biol Reprod, January 1, 2004; 70(1): 244 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. A. Young, C. L. Chaffin, T. A. Molskness, and R. L. Stouffer
Controlled ovulation of the dominant follicle: a critical role for LH in the late follicular phase of the menstrual cycle
Hum. Reprod., November 1, 2003; 18(11): 2257 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J.C. Martinez-Chequer, R.L. Stouffer, T.M. Hazzard, P.E. Patton, and T.A. Molskness
Insulin-Like Growth Factors-1 and -2, but not Hypoxia, Synergize with Gonadotropin Hormone to Promote Vascular Endothelial Growth Factor-A Secretion by Monkey Granulosa Cells from Preovulatory Follicles
Biol Reprod, April 1, 2003; 68(4): 1112 - 1118.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T. M. Hazzard, F. Xu, and R. L. Stouffer
Injection of Soluble Vascular Endothelial Growth Factor Receptor 1 into the Preovulatory Follicle Disrupts Ovulation and Subsequent Luteal Function in Rhesus Monkeys
Biol Reprod, October 1, 2002; 67(4): 1305 - 1312.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K.A. Young, J.D. Hennebold, and R.L. Stouffer
Dynamic expression of mRNAs and proteins for matrix metalloproteinases and their tissue inhibitors in the primate corpus luteum during the menstrual cycle
Mol. Hum. Reprod., September 1, 2002; 8(9): 833 - 840.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. A. LaVoie, D. C. DeSimone, C. Gillio-Meina, and Y. Y. Hui
Cloning and Characterization of Porcine Ovarian Estrogen Receptor {beta} Isoforms
Biol Reprod, March 1, 2002; 66(3): 616 - 623.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. Greco, E. A. Allegretto, M. J. Tetel, and J. D. Blaustein
Coexpression of ER{beta} with ER{alpha} and Progestin Receptor Proteins in the Female Rat Forebrain: Effects of Estradiol Treatment
Endocrinology, December 1, 2001; 142(12): 5172 - 5181.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Cardenas, K.A. Burke, R.M. Bigsby, W.F. Pope, and K.P. Nephew
Estrogen Receptor {beta} in the Sheep Ovary During the Estrous Cycle and Early Pregnancy
Biol Reprod, July 1, 2001; 65(1): 128 - 134.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
S. F. Palter, A. B. Tavares, A. Hourvitz, J. D. Veldhuis, and E. Y. Adashi
Are Estrogens of Import to Primate/Human Ovarian Folliculogenesis?
Endocr. Rev., June 1, 2001; 22(3): 389 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. Hosokawa, U. Ottander, P. Wahlberg, T. Ny, S. Cajander, and I.J. Olofsson
Dominant expression and distribution of oestrogen receptor {beta} over oestrogen receptor {{alpha}} in the human corpus luteum
Mol. Hum. Reprod., February 1, 2001; 7(2): 137 - 145.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
T.M. Hazzard, L.K. Christenson, and R.L. Stouffer
Changes in expression of vascular endothelial growth factor and angiopoietin-1 and -2 in the macaque corpus luteum during the menstrual cycle
Mol. Hum. Reprod., November 1, 2000; 6(11): 993 - 998.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society